APLF_DROME
ID APLF_DROME Reviewed; 255 AA.
AC A8JR14; Q9VF76;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Aprataxin and PNK-like factor;
DE EC=3.1.1.- {ECO:0000269|PubMed:17396150};
DE AltName: Full=Apurinic-apyrimidinic endonuclease APLF;
GN ORFNames=CG6171;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=17396150; DOI=10.1038/sj.emboj.7601663;
RA Kanno S., Kuzuoka H., Sasao S., Hong Z., Lan L., Nakajima S., Yasui A.;
RT "A novel human AP endonuclease with conserved zinc-finger-like motifs
RT involved in DNA strand break responses.";
RL EMBO J. 26:2094-2103(2007).
CC -!- FUNCTION: Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5'
CC exonuclease activities in vitro. {ECO:0000269|PubMed:17396150}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B;
CC IsoId=A8JR14-1; Sequence=Displayed;
CC Name=A;
CC IsoId=A8JR14-2; Sequence=VSP_038137, VSP_038138;
CC -!- SIMILARITY: Belongs to the APLF family. {ECO:0000305}.
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DR EMBL; AE014297; ABW08680.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF55184.1; -; Genomic_DNA.
DR EMBL; BT004895; AAO47873.1; -; mRNA.
DR RefSeq; NP_001097801.1; NM_001104331.2. [A8JR14-1]
DR RefSeq; NP_650455.1; NM_142198.3. [A8JR14-2]
DR AlphaFoldDB; A8JR14; -.
DR BioGRID; 66935; 10.
DR STRING; 7227.FBpp0111839; -.
DR PaxDb; A8JR14; -.
DR DNASU; 41872; -.
DR EnsemblMetazoa; FBtr0083113; FBpp0082567; FBgn0026737. [A8JR14-2]
DR EnsemblMetazoa; FBtr0112926; FBpp0111839; FBgn0026737. [A8JR14-1]
DR GeneID; 41872; -.
DR KEGG; dme:Dmel_CG6171; -.
DR UCSC; CG6171-RA; d. melanogaster.
DR UCSC; CG6171-RB; d. melanogaster. [A8JR14-1]
DR FlyBase; FBgn0026737; CG6171.
DR VEuPathDB; VectorBase:FBgn0026737; -.
DR eggNOG; ENOG502R7QZ; Eukaryota.
DR GeneTree; ENSGT00390000010591; -.
DR HOGENOM; CLU_079469_0_0_1; -.
DR InParanoid; A8JR14; -.
DR OMA; RMSHPPN; -.
DR PhylomeDB; A8JR14; -.
DR BioGRID-ORCS; 41872; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41872; -.
DR PRO; PR:A8JR14; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0026737; Expressed in ovary and 11 other tissues.
DR Genevisible; A8JR14; DM.
DR GO; GO:0005634; C:nucleus; ISS:FlyBase.
DR GO; GO:0035861; C:site of double-strand break; IBA:GO_Central.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IDA:UniProtKB.
DR GO; GO:0052720; F:class II DNA-(apurinic or apyrimidinic site) endonuclease activity; IDA:FlyBase.
DR GO; GO:0003906; F:DNA-(apurinic or apyrimidinic site) endonuclease activity; IDA:UniProtKB.
DR GO; GO:0004520; F:endodeoxyribonuclease activity; IDA:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000737; P:DNA catabolic process, endonucleolytic; IDA:FlyBase.
DR GO; GO:0006302; P:double-strand break repair; ISS:FlyBase.
DR GO; GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IDA:FlyBase.
DR GO; GO:0000012; P:single strand break repair; ISS:FlyBase.
DR InterPro; IPR039253; APLF.
DR InterPro; IPR019406; APLF_PBZ.
DR PANTHER; PTHR21315; PTHR21315; 1.
DR Pfam; PF10283; zf-CCHH; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Hydrolase; Metal-binding; Reference proteome; Repeat;
KW Zinc; Zinc-finger.
FT CHAIN 1..255
FT /note="Aprataxin and PNK-like factor"
FT /id="PRO_0000385300"
FT ZN_FING 121..142
FT /note="PBZ-type 1"
FT ZN_FING 161..182
FT /note="PBZ-type 2"
FT REGION 1..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 131..168
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 195..255
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 35..66
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 94..117
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 133..147
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 232..255
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 186..187
FT /note="FN -> CK (in isoform A)"
FT /evidence="ECO:0000305"
FT /id="VSP_038137"
FT VAR_SEQ 188..255
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000305"
FT /id="VSP_038138"
SQ SEQUENCE 255 AA; 28006 MW; 1CB1AC10524D57F3 CRC64;
MSATDASTAD SGAKRKSSED ITHNCNANFG AENGLRKRVK SEEPVASIKD ETNPEVPMKI
KAEPVENADE PTSTTPAIKI KAEPADNGNS PAAAMVKTEP TNSNAQDAAD ESTVSSSSIR
TSCRFGIRCY RRNPAHRSAE AHPGDQDYRR PNFPAPPLGT PACPFGNACY RRNPVHFQDY
SHPADFNSAQ NIRNRLRQRR AQRQNDDDSG TDEEDEPFGG DNDRDADYRP GADINEDEDD
ELEFDSQPIS GDDYD
