ID   IKZF1_ONCMY             Reviewed;         522 AA.
AC   O13089; O13086; O13087; O13088;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=DNA-binding protein Ikaros;
DE   AltName: Full=Ikaros family zinc finger protein 1;
GN   Name=ikzf1; Synonyms=ikaros;
OS   Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC   Salmonidae; Salmoninae; Oncorhynchus.
OX   NCBI_TaxID=8022;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS IK-1; IK-2; IK-3; IK-4; IK-5; IK-6;
RP   IK-7 AND IK-8), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND SUBCELLULAR
RP   LOCATION.
RC   STRAIN=Shasta; TISSUE=Thymocyte;
RX   PubMed=9394836; DOI=10.1002/eji.1830271143;
RA   Hansen J.D., Strassburger P., du Pasquier L.;
RT   "Conservation of a master hematopoietic switch gene during vertebrate
RT   evolution: isolation and characterization of Ikaros from teleost and
RT   amphibian species.";
RL   Eur. J. Immunol. 27:3049-3058(1997).
CC   -!- FUNCTION: Binds and activates the enhancer (delta-A element) of the
CC       CD3-delta gene. Functions in the specification and the maturation of
CC       the T-lymphocyte. Also interacts with a critical control element in the
CC       TDT (terminal deoxynucleotidyltransferase) promoter as well as with the
CC       promoters for other genes expressed during early stages of B- and T-
CC       cell development. Function is isoform-specific and is modulated by
CC       dominant-negative inactive isoforms (By similarity).
CC       {ECO:0000250|UniProtKB:Q03267, ECO:0000250|UniProtKB:Q13422}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9394836}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=8;
CC       Name=IK-1;
CC         IsoId=O13089-1; Sequence=Displayed;
CC       Name=IK-2;
CC         IsoId=O13089-2; Sequence=VSP_006857;
CC       Name=IK-3;
CC         IsoId=O13089-3; Sequence=VSP_006861;
CC       Name=IK-4;
CC         IsoId=O13089-4; Sequence=VSP_006857, VSP_006860;
CC       Name=IK-5;
CC         IsoId=O13089-5; Sequence=VSP_006859;
CC       Name=IK-6;
CC         IsoId=O13089-6; Sequence=VSP_006858;
CC       Name=IK-7;
CC         IsoId=O13089-7; Sequence=VSP_006862;
CC       Name=IK-8;
CC         IsoId=O13089-8; Sequence=VSP_006857, VSP_006862;
CC   -!- TISSUE SPECIFICITY: Expression mainly limited to thymus, spleen and
CC       pronephros. Very low expression in liver. No expression in testis,
CC       brain, eye and muscle. {ECO:0000269|PubMed:9394836}.
CC   -!- DEVELOPMENTAL STAGE: Expression begins at day 3-4 in the yolk sac and
CC       at day 5-6 in the embryo proper. {ECO:0000269|PubMed:9394836}.
CC   -!- SIMILARITY: Belongs to the Ikaros C2H2-type zinc-finger protein family.
CC       {ECO:0000305}.
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DR   EMBL; U92201; AAB97474.1; -; mRNA.
DR   EMBL; U92200; AAB53434.1; -; mRNA.
DR   EMBL; U92198; AAB53432.1; -; mRNA.
DR   EMBL; U92199; AAB53433.1; -; mRNA.
DR   RefSeq; NP_001118184.1; NM_001124712.1. [O13089-1]
DR   AlphaFoldDB; O13089; -.
DR   SMR; O13089; -.
DR   Ensembl; ENSOMYT00000108048; ENSOMYP00000099558; ENSOMYG00000044996. [O13089-5]
DR   Ensembl; ENSOMYT00000108051; ENSOMYP00000099561; ENSOMYG00000044996. [O13089-6]
DR   GeneID; 100136762; -.
DR   KEGG; omy:100136762; -.
DR   CTD; 10320; -.
DR   GeneTree; ENSGT00940000156782; -.
DR   OrthoDB; 385551at2759; -.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   Pfam; PF00096; zf-C2H2; 2.
DR   SMART; SM00355; ZnF_C2H2; 6.
DR   SUPFAM; SSF57667; SSF57667; 3.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 5.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE   2: Evidence at transcript level;
KW   Activator; Alternative splicing; Developmental protein; DNA-binding;
KW   Metal-binding; Nucleus; Repeat; Transcription; Transcription regulation;
KW   Zinc; Zinc-finger.
FT   CHAIN           1..522
FT                   /note="DNA-binding protein Ikaros"
FT                   /id="PRO_0000047097"
FT   ZN_FING         125..147
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         153..175
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         181..203
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         209..232
FT                   /note="C2H2-type 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         468..490
FT                   /note="C2H2-type 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         496..520
FT                   /note="C2H2-type 6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          1..48
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          96..115
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          379..406
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        379..397
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         55..291
FT                   /note="Missing (in isoform IK-6)"
FT                   /evidence="ECO:0000303|PubMed:9394836"
FT                   /id="VSP_006858"
FT   VAR_SEQ         55..148
FT                   /note="Missing (in isoform IK-2, isoform IK-4 and isoform
FT                   IK-8)"
FT                   /evidence="ECO:0000303|PubMed:9394836"
FT                   /id="VSP_006857"
FT   VAR_SEQ         149..291
FT                   /note="Missing (in isoform IK-5)"
FT                   /evidence="ECO:0000303|PubMed:9394836"
FT                   /id="VSP_006859"
FT   VAR_SEQ         206..291
FT                   /note="Missing (in isoform IK-3)"
FT                   /evidence="ECO:0000303|PubMed:9394836"
FT                   /id="VSP_006861"
FT   VAR_SEQ         206..246
FT                   /note="Missing (in isoform IK-4)"
FT                   /evidence="ECO:0000303|PubMed:9394836"
FT                   /id="VSP_006860"
FT   VAR_SEQ         247..291
FT                   /note="Missing (in isoform IK-7 and isoform IK-8)"
FT                   /evidence="ECO:0000303|PubMed:9394836"
FT                   /id="VSP_006862"
SQ   SEQUENCE   522 AA;  57658 MW;  EE9DA9B2A0ECF88A CRC64;
     MEMEEAQEMS QMPGRDSPPP NDVSEENDEA MPIPEDLSAS SNLQHNNRGD KEGLACNIKV
     EARCDEENGL AIDMMMNGEE EEECAEDLRV LDASGAKVNG SHAGGPDSKG PYSSAGGIRL
     PNGKLKCDIC GIVCIGPNVL MVHKRSHTGE RPFQCTQCGA SFTQKGNLLR HIKLHSGEKP
     FKCHLCNYAC RRRDALSGHL RTHSVGKPHK CAYCGRSYKQ RSSLEEHKER CHNYLQCMGL
     QNSIYTVVKE ESNQNEQRED LSQMGSKRAL VLDRLANNVA KRKSTMPQKF VGEKRFSNIS
     FEGGPGELMQ PHVIDQAINS AINYLGAESL RPLIQTSPTS SDMGVMGSMY PLHKPPAEGH
     GLSAKDSAAE NLLLLAKSKS ASSEKDGSPS HSGQDSTDTE SNNEEKAGVG ASGLIYLTNH
     ITSGVRNGVL PLVKEEQQRQ YEAMRASIEI ASEGFKVLSG EGEQVRAYRC EHCRILFLDH
     VMYTIHMGCH GFRDPFECNL CGHRSQDRYE FSSHMTRGEH RY