IKZF5_MOUSE
ID IKZF5_MOUSE Reviewed; 419 AA.
AC Q8BU00; Q3U549; Q3USG0; Q8BZR5;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Zinc finger protein Pegasus;
DE AltName: Full=Ikaros family zinc finger protein 5;
GN Name=Ikzf5; Synonyms=Zfpn1a5, Znfn1a5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=C57BL/6J, and NOD;
RC TISSUE=Cecum, Heart, Medulla oblongata, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Transcriptional repressor that binds the core 5'GNNTGTNG-3'
CC DNA consensus sequence (By similarity). Involved in megakaryocyte
CC differentiation (By similarity). {ECO:0000250|UniProtKB:Q9H5V7}.
CC -!- SUBUNIT: Self-associates. Interacts with other family members; IKZF1,
CC IKZF2, IKZF3 AND IKZF4 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9H5V7}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8BU00-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BU00-2; Sequence=VSP_027692, VSP_027693;
CC Name=3;
CC IsoId=Q8BU00-3; Sequence=VSP_027694;
CC -!- DOMAIN: The N-terminal zinc fingers are involved in sequence-specific
CC DNA binding and heterotypic associations with other family members.
CC {ECO:0000250}.
CC -!- DOMAIN: C-terminal zinc fingers mediate homodimerization.
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: 'Pegasus' was the winged horse in Greek mythology.
CC -!- SIMILARITY: Belongs to the Ikaros C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
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DR EMBL; AK033716; BAC28443.1; -; mRNA.
DR EMBL; AK088289; BAC40260.1; -; mRNA.
DR EMBL; AK140401; BAE24372.1; -; mRNA.
DR EMBL; AK153883; BAE32231.1; -; mRNA.
DR EMBL; AK169384; BAE41131.1; -; mRNA.
DR EMBL; BC048183; AAH48183.1; -; mRNA.
DR EMBL; BC058537; AAH58537.1; -; mRNA.
DR EMBL; BC089455; AAH89455.1; -; mRNA.
DR CCDS; CCDS21915.1; -. [Q8BU00-1]
DR RefSeq; NP_780324.2; NM_175115.4. [Q8BU00-1]
DR RefSeq; XP_006508185.1; XM_006508122.3. [Q8BU00-1]
DR AlphaFoldDB; Q8BU00; -.
DR BioGRID; 211973; 1.
DR STRING; 10090.ENSMUSP00000035583; -.
DR iPTMnet; Q8BU00; -.
DR PhosphoSitePlus; Q8BU00; -.
DR EPD; Q8BU00; -.
DR MaxQB; Q8BU00; -.
DR PaxDb; Q8BU00; -.
DR PeptideAtlas; Q8BU00; -.
DR PRIDE; Q8BU00; -.
DR ProteomicsDB; 267117; -. [Q8BU00-1]
DR ProteomicsDB; 267118; -. [Q8BU00-2]
DR ProteomicsDB; 267119; -. [Q8BU00-3]
DR Antibodypedia; 9078; 57 antibodies from 18 providers.
DR DNASU; 67143; -.
DR Ensembl; ENSMUST00000046306; ENSMUSP00000035583; ENSMUSG00000040167. [Q8BU00-1]
DR Ensembl; ENSMUST00000121033; ENSMUSP00000113714; ENSMUSG00000040167. [Q8BU00-3]
DR GeneID; 67143; -.
DR KEGG; mmu:67143; -.
DR UCSC; uc009kbj.1; mouse. [Q8BU00-1]
DR CTD; 64376; -.
DR MGI; MGI:1914393; Ikzf5.
DR VEuPathDB; HostDB:ENSMUSG00000040167; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000155035; -.
DR HOGENOM; CLU_734778_0_0_1; -.
DR InParanoid; Q8BU00; -.
DR OMA; THNQRNY; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q8BU00; -.
DR TreeFam; TF331860; -.
DR BioGRID-ORCS; 67143; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Ikzf5; mouse.
DR PRO; PR:Q8BU00; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q8BU00; protein.
DR Bgee; ENSMUSG00000040167; Expressed in manus and 232 other tissues.
DR ExpressionAtlas; Q8BU00; baseline and differential.
DR Genevisible; Q8BU00; MM.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0019904; F:protein domain specific binding; ISO:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0008270; F:zinc ion binding; ISO:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR SMART; SM00355; ZnF_C2H2; 5.
DR SUPFAM; SSF57667; SSF57667; 3.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Isopeptide bond; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation; Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..419
FT /note="Zinc finger protein Pegasus"
FT /id="PRO_0000299472"
FT ZN_FING 82..104
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 110..132
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 138..161
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 364..386
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 392..416
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 36..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 262..284
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 297..356
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 297..352
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 5
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9H5V7"
FT CROSSLNK 185
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9H5V7"
FT VAR_SEQ 1..67
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_027692"
FT VAR_SEQ 68..103
FT /note="GMLVDGFERTFDGKLKCRYCNYASKGTARLIEHIRI -> MHFCKEFYFCIS
FT SHMQWTNMKSPMSELLLQSLPCLS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_027693"
FT VAR_SEQ 106..258
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_027694"
FT CONFLICT 9
FT /note="L -> W (in Ref. 1; BAE32231)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 419 AA; 46400 MW; BD153175F261D97B CRC64;
MGEKKPEPLD FVKDFQEYLT QQTHHVNMIS GSVSGDKEAE TLQGAGTDGD QNGLDHPSVE
VSLDENSGML VDGFERTFDG KLKCRYCNYA SKGTARLIEH IRIHTGEKPH RCHLCPFASA
YERHLEAHMR SHTGEKPYKC ELCSFRCSDR SNLSHHRRRK HKMVPIKGTR SSLSSKKMWG
VLQKKTSNLG YSRRALINLS PPSMVVQKPD YLNDFTHEIP NIQTDSYEAM AKTTPTGGLP
RDPQELMVDN PLNQLSTLAG QLSSLPPENQ NPASPDVDAC PDEKPFMIQQ PSAQAVVSAV
SASIPQSSSP TSPEPRPSHS QRNYSPVAGP SSEPSAHTST PSIGNSQPST PAPTLPVQDP
QLLHHCQHCD VYFADNVLYT VHMGCHGYDS PFQCNVCGCK CKDKYDFACH FARGQHNQH
