ID   IL10_CAVPO              Reviewed;         178 AA.
AC   Q9Z1Y5;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Interleukin-10;
DE            Short=IL-10;
DE   AltName: Full=Cytokine synthesis inhibitory factor;
DE            Short=CSIF;
DE   Flags: Precursor;
GN   Name=IL10;
OS   Cavia porcellus (Guinea pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Caviidae;
OC   Cavia.
OX   NCBI_TaxID=10141;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10025978; DOI=10.1016/s1043-4666(98)90002-3;
RA   Scarozza A.M., Ramsingh A.I., Wicher V., Wicher K.;
RT   "Spontaneous cytokine gene expression in normal guinea pig blood and
RT   tissues.";
RL   Cytokine 10:851-859(1998).
CC   -!- FUNCTION: Major immune regulatory cytokine that acts on many cells of
CC       the immune system where it has profound anti-inflammatory functions,
CC       limiting excessive tissue disruption caused by inflammation.
CC       Mechanistically, IL10 binds to its heterotetrameric receptor comprising
CC       IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of
CC       STAT3. In turn, STAT3 translocates to the nucleus where it drives
CC       expression of anti-inflammatory mediators. Targets antigen-presenting
CC       cells (APCs) such as macrophages and monocytes and inhibits their
CC       release of pro-inflammatory cytokines including granulocyte-macrophage
CC       colony-stimulating factor /GM-CSF, granulocyte colony-stimulating
CC       factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha.
CC       Interferes also with antigen presentation by reducing the expression of
CC       MHC-class II and co-stimulatory molecules, thereby inhibiting their
CC       ability to induce T cell activation (By similarity). In addition,
CC       controls the inflammatory response of macrophages by reprogramming
CC       essential metabolic pathways including mTOR signaling (By similarity).
CC       {ECO:0000250|UniProtKB:P18893, ECO:0000250|UniProtKB:P22301}.
CC   -!- SUBUNIT: Homodimer. Interacts with IL10RA and IL10RB.
CC       {ECO:0000250|UniProtKB:P22301}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P22301}.
CC   -!- SIMILARITY: Belongs to the IL-10 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF097510; AAC83808.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9Z1Y5; -.
DR   SMR; Q9Z1Y5; -.
DR   InParanoid; Q9Z1Y5; -.
DR   Proteomes; UP000005447; Unassembled WGS sequence.
DR   GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR   GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR   GO; GO:0006955; P:immune response; IEA:InterPro.
DR   GO; GO:0030889; P:negative regulation of B cell proliferation; ISS:UniProtKB.
DR   GO; GO:0002719; P:negative regulation of cytokine production involved in immune response; ISS:UniProtKB.
DR   GO; GO:0050728; P:negative regulation of inflammatory response; ISS:UniProtKB.
DR   GO; GO:0032715; P:negative regulation of interleukin-6 production; ISS:UniProtKB.
DR   GO; GO:0051045; P:negative regulation of membrane protein ectodomain proteolysis; ISS:UniProtKB.
DR   GO; GO:0002904; P:positive regulation of B cell apoptotic process; ISS:UniProtKB.
DR   GO; GO:0001819; P:positive regulation of cytokine production; ISS:UniProtKB.
DR   GO; GO:0051091; P:positive regulation of DNA-binding transcription factor activity; ISS:UniProtKB.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0051384; P:response to glucocorticoid; ISS:UniProtKB.
DR   GO; GO:0002237; P:response to molecule of bacterial origin; ISS:UniProtKB.
DR   Gene3D; 1.20.1250.10; -; 1.
DR   InterPro; IPR009079; 4_helix_cytokine-like_core.
DR   InterPro; IPR000098; IL-10.
DR   InterPro; IPR020443; IL-10/19/20/22/24/26_fam.
DR   InterPro; IPR020423; IL-10_CS.
DR   Pfam; PF00726; IL10; 1.
DR   PRINTS; PR01294; INTRLEUKIN10.
DR   SMART; SM00188; IL10; 1.
DR   SUPFAM; SSF47266; SSF47266; 1.
DR   PROSITE; PS00520; INTERLEUKIN_10; 1.
PE   3: Inferred from homology;
KW   Cytokine; Disulfide bond; Glycoprotein; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..178
FT                   /note="Interleukin-10"
FT                   /id="PRO_0000015355"
FT   CARBOHYD        67
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        134
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        30..126
FT                   /evidence="ECO:0000250"
FT   DISULFID        80..132
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   178 AA;  20238 MW;  9D2DBDA70DB1CE6D CRC64;
     MPGSALLCCL ALLAGVKASQ GTNTQSEDSC AHFPAGLPHM LRELRAAFGR VKTFFQTQDQ
     LDNVLLNKSL LEDFKGYLGC QALSEMIQFY LVEVMPQAEK HGPEIKEHLN SLGEKLKTLR
     MRLRRCHRFL PCENKSKAVE QVKSDFNKLQ DQGVYKAMNE FDIFINCIEA YMMIKMKS