IL10_PIG
ID IL10_PIG Reviewed; 175 AA.
AC Q29055;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Interleukin-10;
DE Short=IL-10;
DE AltName: Full=Cytokine synthesis inhibitory factor;
DE Short=CSIF;
DE Flags: Precursor;
GN Name=IL10;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Spleen;
RX PubMed=7708727; DOI=10.1073/pnas.92.7.2800;
RA Blancho G., Gianello P., Germana S., Baetscher M., Sachs D.H., Leguern C.;
RT "Molecular identification of porcine interleukin 10: regulation of
RT expression in a kidney allograft model.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:2800-2804(1995).
CC -!- FUNCTION: Major immune regulatory cytokine that acts on many cells of
CC the immune system where it has profound anti-inflammatory functions,
CC limiting excessive tissue disruption caused by inflammation.
CC Mechanistically, IL10 binds to its heterotetrameric receptor comprising
CC IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of
CC STAT3. In turn, STAT3 translocates to the nucleus where it drives
CC expression of anti-inflammatory mediators. Targets antigen-presenting
CC cells (APCs) such as macrophages and monocytes and inhibits their
CC release of pro-inflammatory cytokines including granulocyte-macrophage
CC colony-stimulating factor /GM-CSF, granulocyte colony-stimulating
CC factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha.
CC Interferes also with antigen presentation by reducing the expression of
CC MHC-class II and co-stimulatory molecules, thereby inhibiting their
CC ability to induce T cell activation (By similarity). In addition,
CC controls the inflammatory response of macrophages by reprogramming
CC essential metabolic pathways including mTOR signaling (By similarity).
CC {ECO:0000250|UniProtKB:P18893, ECO:0000250|UniProtKB:P22301}.
CC -!- SUBUNIT: Homodimer. Interacts with IL10RA and IL10RB.
CC {ECO:0000250|UniProtKB:P22301}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P22301}.
CC -!- SIMILARITY: Belongs to the IL-10 family. {ECO:0000305}.
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DR EMBL; L20001; AAA74410.1; -; mRNA.
DR PIR; I46591; I46591.
DR RefSeq; NP_999206.1; NM_214041.1.
DR AlphaFoldDB; Q29055; -.
DR SMR; Q29055; -.
DR STRING; 9823.ENSSSCP00000016594; -.
DR PaxDb; Q29055; -.
DR Ensembl; ENSSSCT00005007269; ENSSSCP00005004226; ENSSSCG00005004875.
DR GeneID; 397106; -.
DR KEGG; ssc:397106; -.
DR CTD; 3586; -.
DR eggNOG; ENOG502S22U; Eukaryota.
DR HOGENOM; CLU_127747_0_0_1; -.
DR InParanoid; Q29055; -.
DR OMA; MIQFYLV; -.
DR OrthoDB; 1389566at2759; -.
DR TreeFam; TF333253; -.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Unplaced.
DR Genevisible; Q29055; SS.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0046983; F:protein dimerization activity; IEA:Ensembl.
DR GO; GO:0060670; P:branching involved in labyrinthine layer morphogenesis; IEA:Ensembl.
DR GO; GO:0035729; P:cellular response to hepatocyte growth factor stimulus; IEA:Ensembl.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IEA:Ensembl.
DR GO; GO:0002439; P:chronic inflammatory response to antigenic stimulus; IEA:Ensembl.
DR GO; GO:0042742; P:defense response to bacterium; IEA:Ensembl.
DR GO; GO:0042832; P:defense response to protozoan; IEA:Ensembl.
DR GO; GO:0010507; P:negative regulation of autophagy; IEA:Ensembl.
DR GO; GO:0030889; P:negative regulation of B cell proliferation; IEA:Ensembl.
DR GO; GO:0002875; P:negative regulation of chronic inflammatory response to antigenic stimulus; IEA:Ensembl.
DR GO; GO:0060302; P:negative regulation of cytokine activity; IEA:Ensembl.
DR GO; GO:0002719; P:negative regulation of cytokine production involved in immune response; IEA:Ensembl.
DR GO; GO:2000352; P:negative regulation of endothelial cell apoptotic process; IEA:Ensembl.
DR GO; GO:0034115; P:negative regulation of heterotypic cell-cell adhesion; IEA:Ensembl.
DR GO; GO:1903208; P:negative regulation of hydrogen peroxide-induced neuron death; IEA:Ensembl.
DR GO; GO:0032689; P:negative regulation of interferon-gamma production; IEA:Ensembl.
DR GO; GO:0032695; P:negative regulation of interleukin-12 production; IEA:Ensembl.
DR GO; GO:0032715; P:negative regulation of interleukin-6 production; IEA:Ensembl.
DR GO; GO:0051045; P:negative regulation of membrane protein ectodomain proteolysis; IEA:Ensembl.
DR GO; GO:0045347; P:negative regulation of MHC class II biosynthetic process; IEA:Ensembl.
DR GO; GO:0030886; P:negative regulation of myeloid dendritic cell activation; IEA:Ensembl.
DR GO; GO:0032720; P:negative regulation of tumor necrosis factor production; IEA:Ensembl.
DR GO; GO:1904706; P:negative regulation of vascular associated smooth muscle cell proliferation; IEA:Ensembl.
DR GO; GO:0002904; P:positive regulation of B cell apoptotic process; IEA:Ensembl.
DR GO; GO:0045787; P:positive regulation of cell cycle; IEA:Ensembl.
DR GO; GO:0001819; P:positive regulation of cytokine production; IEA:Ensembl.
DR GO; GO:0051091; P:positive regulation of DNA-binding transcription factor activity; IEA:Ensembl.
DR GO; GO:0001938; P:positive regulation of endothelial cell proliferation; IEA:Ensembl.
DR GO; GO:0002639; P:positive regulation of immunoglobulin production; IEA:Ensembl.
DR GO; GO:0045348; P:positive regulation of MHC class II biosynthetic process; IEA:Ensembl.
DR GO; GO:1902895; P:positive regulation of miRNA transcription; IEA:Ensembl.
DR GO; GO:1900100; P:positive regulation of plasma cell differentiation; IEA:Ensembl.
DR GO; GO:0046427; P:positive regulation of receptor signaling pathway via JAK-STAT; IEA:Ensembl.
DR GO; GO:2000273; P:positive regulation of signaling receptor activity; IEA:Ensembl.
DR GO; GO:1903672; P:positive regulation of sprouting angiogenesis; IEA:Ensembl.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR GO; GO:1903034; P:regulation of response to wounding; IEA:Ensembl.
DR GO; GO:0051384; P:response to glucocorticoid; IEA:Ensembl.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR000098; IL-10.
DR InterPro; IPR020443; IL-10/19/20/22/24/26_fam.
DR InterPro; IPR020423; IL-10_CS.
DR Pfam; PF00726; IL10; 1.
DR PRINTS; PR01294; INTRLEUKIN10.
DR SMART; SM00188; IL10; 1.
DR SUPFAM; SSF47266; SSF47266; 1.
DR PROSITE; PS00520; INTERLEUKIN_10; 1.
PE 2: Evidence at transcript level;
KW Cytokine; Disulfide bond; Glycoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..175
FT /note="Interleukin-10"
FT /id="PRO_0000015370"
FT CARBOHYD 130
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 26..122
FT /evidence="ECO:0000250"
FT DISULFID 76..128
FT /evidence="ECO:0000250"
SQ SEQUENCE 175 AA; 19934 MW; DB85B9984C1570BF CRC64;
MPSSALLYCL IFLAGVAASI KSENSCIHFP TSLPHMLREL RAAFGPVKSF FQTKDQMGDL
LLTGSLLEDF KGYLGCQALS EMIQFYLEDV MPKAESDGED IKEHVNSLGE KLKTLRLRLR
RCHQFLPCEN KSKAVEEVKS AFSKLQERGV YKAMGEFDIF INYIEAYMTM KMRKN
