IL10_RAT
ID IL10_RAT Reviewed; 178 AA.
AC P29456; Q63263;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Interleukin-10;
DE Short=IL-10;
DE AltName: Full=Cytokine synthesis inhibitory factor;
DE Short=CSIF;
DE Flags: Precursor;
GN Name=Il10; Synonyms=Il-10;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1280414; DOI=10.1016/0006-291x(92)91516-s;
RA Goodman R.E., Oblak J., Bell R.G.;
RT "Synthesis and characterization of rat interleukin-10 (IL-10) cDNA clones
RT from the RNA of cultured OX8-OX22-thoracic duct T cells.";
RL Biochem. Biophys. Res. Commun. 189:1-7(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Fischer 344; TISSUE=Macrophage;
RX PubMed=8484757; DOI=10.1006/bbrc.1993.1436;
RA Feng L., Tang W.W., Chang J.C.C., Wilson C.B.;
RT "Molecular cloning of rat cytokine synthesis inhibitory factor (IL-10) cDNA
RT and expression in spleen and macrophages.";
RL Biochem. Biophys. Res. Commun. 192:452-458(1993).
CC -!- FUNCTION: Major immune regulatory cytokine that acts on many cells of
CC the immune system where it has profound anti-inflammatory functions,
CC limiting excessive tissue disruption caused by inflammation.
CC Mechanistically, IL10 binds to its heterotetrameric receptor comprising
CC IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of
CC STAT3. In turn, STAT3 translocates to the nucleus where it drives
CC expression of anti-inflammatory mediators. Targets antigen-presenting
CC cells (APCs) such as macrophages and monocytes and inhibits their
CC release of pro-inflammatory cytokines including granulocyte-macrophage
CC colony-stimulating factor /GM-CSF, granulocyte colony-stimulating
CC factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha.
CC Interferes also with antigen presentation by reducing the expression of
CC MHC-class II and co-stimulatory molecules, thereby inhibiting their
CC ability to induce T cell activation (By similarity). In addition,
CC controls the inflammatory response of macrophages by reprogramming
CC essential metabolic pathways including mTOR signaling (By similarity).
CC {ECO:0000250|UniProtKB:P18893, ECO:0000250|UniProtKB:P22301}.
CC -!- SUBUNIT: Homodimer. Interacts with IL10RA and IL10RB.
CC {ECO:0000250|UniProtKB:P22301}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P22301}.
CC -!- SIMILARITY: Belongs to the IL-10 family. {ECO:0000305}.
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DR EMBL; L02926; AAA41425.1; -; mRNA.
DR EMBL; X60675; CAA43090.1; -; mRNA.
DR PIR; JN0475; JN0475.
DR RefSeq; NP_036986.2; NM_012854.2.
DR AlphaFoldDB; P29456; -.
DR SMR; P29456; -.
DR STRING; 10116.ENSRNOP00000006246; -.
DR GlyGen; P29456; 2 sites.
DR PaxDb; P29456; -.
DR Ensembl; ENSRNOT00000006246; ENSRNOP00000006246; ENSRNOG00000004647.
DR GeneID; 25325; -.
DR KEGG; rno:25325; -.
DR UCSC; RGD:2886; rat.
DR CTD; 3586; -.
DR RGD; 2886; Il10.
DR eggNOG; ENOG502S22U; Eukaryota.
DR GeneTree; ENSGT00950000183124; -.
DR HOGENOM; CLU_127747_0_0_1; -.
DR InParanoid; P29456; -.
DR OMA; MIQFYLV; -.
DR OrthoDB; 1316775at2759; -.
DR PhylomeDB; P29456; -.
DR TreeFam; TF333253; -.
DR PRO; PR:P29456; -.
DR Proteomes; UP000002494; Chromosome 13.
DR Bgee; ENSRNOG00000004647; Expressed in ileum and 6 other tissues.
DR Genevisible; P29456; RN.
DR GO; GO:0005615; C:extracellular space; IDA:RGD.
DR GO; GO:0005125; F:cytokine activity; ISO:RGD.
DR GO; GO:0005141; F:interleukin-10 receptor binding; IDA:RGD.
DR GO; GO:0046983; F:protein dimerization activity; ISO:RGD.
DR GO; GO:0007568; P:aging; IEP:RGD.
DR GO; GO:0060670; P:branching involved in labyrinthine layer morphogenesis; ISO:RGD.
DR GO; GO:0071392; P:cellular response to estradiol stimulus; IEP:RGD.
DR GO; GO:0035729; P:cellular response to hepatocyte growth factor stimulus; ISO:RGD.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; ISO:RGD.
DR GO; GO:0002439; P:chronic inflammatory response to antigenic stimulus; IEA:Ensembl.
DR GO; GO:0042742; P:defense response to bacterium; ISO:RGD.
DR GO; GO:0042832; P:defense response to protozoan; ISO:RGD.
DR GO; GO:0006955; P:immune response; IMP:RGD.
DR GO; GO:0097421; P:liver regeneration; IEP:RGD.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:RGD.
DR GO; GO:0010507; P:negative regulation of autophagy; ISO:RGD.
DR GO; GO:0030889; P:negative regulation of B cell proliferation; ISS:UniProtKB.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:RGD.
DR GO; GO:0002875; P:negative regulation of chronic inflammatory response to antigenic stimulus; ISO:RGD.
DR GO; GO:0060302; P:negative regulation of cytokine activity; ISO:RGD.
DR GO; GO:0001818; P:negative regulation of cytokine production; IMP:RGD.
DR GO; GO:0002719; P:negative regulation of cytokine production involved in immune response; ISS:UniProtKB.
DR GO; GO:2000352; P:negative regulation of endothelial cell apoptotic process; ISO:RGD.
DR GO; GO:0034115; P:negative regulation of heterotypic cell-cell adhesion; ISO:RGD.
DR GO; GO:1903208; P:negative regulation of hydrogen peroxide-induced neuron death; ISO:RGD.
DR GO; GO:0050728; P:negative regulation of inflammatory response; IDA:RGD.
DR GO; GO:0032689; P:negative regulation of interferon-gamma production; ISO:RGD.
DR GO; GO:0032695; P:negative regulation of interleukin-12 production; ISO:RGD.
DR GO; GO:0032715; P:negative regulation of interleukin-6 production; ISS:UniProtKB.
DR GO; GO:0051045; P:negative regulation of membrane protein ectodomain proteolysis; ISS:UniProtKB.
DR GO; GO:0045347; P:negative regulation of MHC class II biosynthetic process; ISO:RGD.
DR GO; GO:0030886; P:negative regulation of myeloid dendritic cell activation; ISO:RGD.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; IDA:RGD.
DR GO; GO:0045019; P:negative regulation of nitric oxide biosynthetic process; IDA:RGD.
DR GO; GO:1904057; P:negative regulation of sensory perception of pain; IDA:RGD.
DR GO; GO:0032720; P:negative regulation of tumor necrosis factor production; IDA:RGD.
DR GO; GO:1904706; P:negative regulation of vascular associated smooth muscle cell proliferation; ISO:RGD.
DR GO; GO:0002904; P:positive regulation of B cell apoptotic process; ISS:UniProtKB.
DR GO; GO:0045787; P:positive regulation of cell cycle; ISO:RGD.
DR GO; GO:0001819; P:positive regulation of cytokine production; ISS:UniProtKB.
DR GO; GO:0051091; P:positive regulation of DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0001938; P:positive regulation of endothelial cell proliferation; ISO:RGD.
DR GO; GO:0002639; P:positive regulation of immunoglobulin production; ISO:RGD.
DR GO; GO:0043032; P:positive regulation of macrophage activation; IDA:RGD.
DR GO; GO:0045348; P:positive regulation of MHC class II biosynthetic process; ISO:RGD.
DR GO; GO:1902895; P:positive regulation of miRNA transcription; ISO:RGD.
DR GO; GO:1900100; P:positive regulation of plasma cell differentiation; ISO:RGD.
DR GO; GO:0046427; P:positive regulation of receptor signaling pathway via JAK-STAT; ISO:RGD.
DR GO; GO:2000273; P:positive regulation of signaling receptor activity; ISO:RGD.
DR GO; GO:1903672; P:positive regulation of sprouting angiogenesis; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; ISO:RGD.
DR GO; GO:1903034; P:regulation of response to wounding; ISO:RGD.
DR GO; GO:0051930; P:regulation of sensory perception of pain; IDA:RGD.
DR GO; GO:0050807; P:regulation of synapse organization; IDA:RGD.
DR GO; GO:0014823; P:response to activity; IEP:RGD.
DR GO; GO:0034465; P:response to carbon monoxide; IEP:RGD.
DR GO; GO:0051384; P:response to glucocorticoid; IEP:RGD.
DR GO; GO:0014854; P:response to inactivity; IEP:RGD.
DR GO; GO:0032868; P:response to insulin; IEP:RGD.
DR GO; GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
DR GO; GO:0002237; P:response to molecule of bacterial origin; ISS:UniProtKB.
DR GO; GO:0010033; P:response to organic substance; IEP:RGD.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IEP:RGD.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR000098; IL-10.
DR InterPro; IPR020443; IL-10/19/20/22/24/26_fam.
DR InterPro; IPR020423; IL-10_CS.
DR Pfam; PF00726; IL10; 1.
DR PRINTS; PR01294; INTRLEUKIN10.
DR SMART; SM00188; IL10; 1.
DR SUPFAM; SSF47266; SSF47266; 1.
DR PROSITE; PS00520; INTERLEUKIN_10; 1.
PE 2: Evidence at transcript level;
KW Cytokine; Disulfide bond; Glycoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..178
FT /note="Interleukin-10"
FT /id="PRO_0000015372"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 134
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 30..126
FT /evidence="ECO:0000250"
FT DISULFID 80..132
FT /evidence="ECO:0000250"
FT CONFLICT 2
FT /note="P -> L (in Ref. 2; CAA43090)"
FT /evidence="ECO:0000305"
FT CONFLICT 65
FT /note="L -> V (in Ref. 2; CAA43090)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 178 AA; 20426 MW; 0225AA6C63DAB70B CRC64;
MPGSALLCCL LLLAGVKTSK GHSIRGDNNC THFPVSQTHM LRELRAAFSQ VKTFFQKKDQ
LDNILLTDSL LQDFKGYLGC QALSEMIKFY LVEVMPQAEN HGPEIKEHLN SLGEKLKTLW
IQLRRCHRFL PCENKSKAVE QVKNDFNKLQ DKGVYKAMNE FDIFINCIEA YVTLKMKN
