IL10_SHEEP
ID IL10_SHEEP Reviewed; 177 AA.
AC Q29408;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Interleukin-10;
DE Short=IL-10;
DE AltName: Full=Cytokine synthesis inhibitory factor;
DE Short=CSIF;
DE Flags: Precursor;
GN Name=IL10;
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Macrophage;
RX PubMed=7622047; DOI=10.1016/0378-1119(95)00123-n;
RA Martin H.M., Nash A.D., Andrews A.E.;
RT "Cloning and characterisation of an ovine interleukin-10-encoding cDNA.";
RL Gene 159:187-191(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Macrophage;
RX PubMed=7959030; DOI=10.1016/0378-1119(94)90189-9;
RA Dutia B.M., Hunt P., Sargan D.R., Dalziel R.G., Hopkins J.;
RT "Sequence of the sheep interleukin-10-encoding cDNA.";
RL Gene 149:393-394(1994).
CC -!- FUNCTION: Major immune regulatory cytokine that acts on many cells of
CC the immune system where it has profound anti-inflammatory functions,
CC limiting excessive tissue disruption caused by inflammation.
CC Mechanistically, IL10 binds to its heterotetrameric receptor comprising
CC IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of
CC STAT3. In turn, STAT3 translocates to the nucleus where it drives
CC expression of anti-inflammatory mediators. Targets antigen-presenting
CC cells (APCs) such as macrophages and monocytes and inhibits their
CC release of pro-inflammatory cytokines including granulocyte-macrophage
CC colony-stimulating factor /GM-CSF, granulocyte colony-stimulating
CC factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha.
CC Interferes also with antigen presentation by reducing the expression of
CC MHC-class II and co-stimulatory molecules, thereby inhibiting their
CC ability to induce T cell activation (By similarity). In addition,
CC controls the inflammatory response of macrophages by reprogramming
CC essential metabolic pathways including mTOR signaling (By similarity).
CC {ECO:0000250|UniProtKB:P18893, ECO:0000250|UniProtKB:P22301}.
CC -!- SUBUNIT: Homodimer. Interacts with IL10RA and IL10RB.
CC {ECO:0000250|UniProtKB:P22301}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P22301}.
CC -!- SIMILARITY: Belongs to the IL-10 family. {ECO:0000305}.
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DR EMBL; U11421; AAC13768.1; -; mRNA.
DR EMBL; Z29362; CAA82546.1; -; mRNA.
DR RefSeq; NP_001009327.1; NM_001009327.1.
DR AlphaFoldDB; Q29408; -.
DR SMR; Q29408; -.
DR STRING; 9940.ENSOARP00000006744; -.
DR GeneID; 443342; -.
DR KEGG; oas:443342; -.
DR CTD; 3586; -.
DR eggNOG; ENOG502S22U; Eukaryota.
DR OrthoDB; 1389566at2759; -.
DR Proteomes; UP000002356; Unplaced.
DR GO; GO:0005576; C:extracellular region; NAS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IDA:AgBase.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0005141; F:interleukin-10 receptor binding; NAS:UniProtKB.
DR GO; GO:0006955; P:immune response; IEA:InterPro.
DR GO; GO:0030889; P:negative regulation of B cell proliferation; ISS:UniProtKB.
DR GO; GO:0002719; P:negative regulation of cytokine production involved in immune response; ISS:UniProtKB.
DR GO; GO:0050728; P:negative regulation of inflammatory response; ISS:UniProtKB.
DR GO; GO:0032687; P:negative regulation of interferon-alpha production; IDA:AgBase.
DR GO; GO:0032715; P:negative regulation of interleukin-6 production; ISS:UniProtKB.
DR GO; GO:0051045; P:negative regulation of membrane protein ectodomain proteolysis; ISS:UniProtKB.
DR GO; GO:0002904; P:positive regulation of B cell apoptotic process; ISS:UniProtKB.
DR GO; GO:0001819; P:positive regulation of cytokine production; ISS:UniProtKB.
DR GO; GO:0051091; P:positive regulation of DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0051384; P:response to glucocorticoid; ISS:UniProtKB.
DR GO; GO:0002237; P:response to molecule of bacterial origin; ISS:UniProtKB.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR000098; IL-10.
DR InterPro; IPR020443; IL-10/19/20/22/24/26_fam.
DR InterPro; IPR020423; IL-10_CS.
DR Pfam; PF00726; IL10; 1.
DR PRINTS; PR01294; INTRLEUKIN10.
DR SMART; SM00188; IL10; 1.
DR SUPFAM; SSF47266; SSF47266; 1.
DR PROSITE; PS00520; INTERLEUKIN_10; 1.
PE 2: Evidence at transcript level;
KW Cytokine; Disulfide bond; Glycoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..177
FT /note="Interleukin-10"
FT /id="PRO_0000015373"
FT CARBOHYD 135
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 31..127
FT /evidence="ECO:0000250"
FT DISULFID 81..133
FT /evidence="ECO:0000250"
SQ SEQUENCE 177 AA; 20189 MW; EF60025499E29BED CRC64;
MPSSSAVLCC LVFLAGVAAS RDASTLSDSS CTHFPASLPH MLRDVRAAFG KVKTFFQMKD
QLNSMLLTQS LLDDFKGYLG CQALSEMIQF YLEEVMPQAE NHGPDIKEHV NSLGEKLKTL
RLRLRRCHRF LPCENKSKAV EQVKRVFNML QERGVYKAMS EFDIFINYIE SYMTTKM
