IL12B_BUBCA
ID IL12B_BUBCA Reviewed; 327 AA.
AC Q2PE76;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 07-FEB-2006, sequence version 1.
DT 25-MAY-2022, entry version 59.
DE RecName: Full=Interleukin-12 subunit beta;
DE Short=IL-12B;
DE AltName: Full=Cytotoxic lymphocyte maturation factor 40 kDa subunit;
DE Short=CLMF p40;
DE AltName: Full=IL-12 subunit p40;
DE Flags: Precursor;
GN Name=IL12B;
OS Bubalus carabanensis (Swamp type water buffalo) (Bubalus bubalis
OS carabanensis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bubalus.
OX NCBI_TaxID=346063;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=16870267; DOI=10.1016/j.vetimm.2006.06.005;
RA Mingala C.N., Odbileg R., Konnai S., Ohashi K., Onuma M.;
RT "Comparative assessment of Th1 and Th2 cytokines of swamp type buffalo and
RT other bubaline breeds by molecular cloning, sequencing and phylogenetics.";
RL Vet. Immunol. Immunopathol. 113:348-356(2006).
CC -!- FUNCTION: Cytokine that can act as a growth factor for activated T and
CC NK cells, enhance the lytic activity of NK/lymphokine-activated killer
CC cells, and stimulate the production of IFN-gamma by resting PBMC.
CC {ECO:0000250}.
CC -!- SUBUNIT: Disulfide-bonded heterodimer (IL-12 p70) of 40 kDa and 35 kDa
CC subunits. Also secreted as a monomer (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the IL-12B family. {ECO:0000305}.
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DR EMBL; AB246273; BAE75850.1; -; mRNA.
DR AlphaFoldDB; Q2PE76; -.
DR SMR; Q2PE76; -.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0004896; F:cytokine receptor activity; IEA:InterPro.
DR CDD; cd00063; FN3; 1.
DR Gene3D; 2.60.40.10; -; 3.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR003530; Hematopoietin_rcpt_L_F3_CS.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003598; Ig_sub2.
DR InterPro; IPR015528; IL-12_beta.
DR InterPro; IPR019482; IL-12_beta_cen-dom.
DR PANTHER; PTHR23036:SF156; PTHR23036:SF156; 1.
DR Pfam; PF10420; IL12p40_C; 1.
DR PIRSF; PIRSF038007; IL_12_beta; 1.
DR PRINTS; PR01928; INTRLEUKN12B.
DR SMART; SM00408; IGc2; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR SUPFAM; SSF49265; SSF49265; 2.
DR PROSITE; PS50853; FN3; 1.
DR PROSITE; PS01354; HEMATOPO_REC_L_F3; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 2: Evidence at transcript level;
KW Cytokine; Disulfide bond; Glycoprotein; Immunoglobulin domain; Secreted;
KW Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000250"
FT CHAIN 23..327
FT /note="Interleukin-12 subunit beta"
FT /id="PRO_0000254897"
FT DOMAIN 23..106
FT /note="Ig-like C2-type"
FT DOMAIN 238..327
FT /note="Fibronectin type-III"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT CARBOHYD 223
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 50..90
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 200
FT /note="Interchain (with C-98 in IL12A)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
SQ SEQUENCE 327 AA; 37022 MW; E406253B02F07F5B CRC64;
MHPQQLVVSW FSLVLLASPI VAIWELEKNV YIVELDWYPD APGETVVLTC DTPEEDGITW
TSDQSSEVLG SGKTLTIQVK EFGDAGQYTC HKGGEALSRS LLLLHKKEDG IWSTDILKDQ
KEPKAKSFLK CEAKDYSGHF TCWWLTAIST DLKFSVKSSR GSSDPRGVTC GAASLSAEKV
SVDHREYNKY TVECQEGSTC PAAEESLLIE VVVEAVHKLK YENYTSSFFI RDIIKPDPPK
NLQLRPLKNS RQVEVSWEYP DTWSTPHSYF SLTFCVQVQG KNKREKKLFM DQTSAKVTCH
KDANVRVQAR DRYYSSFWSE WASVSCS
