IL12B_FELCA
ID IL12B_FELCA Reviewed; 329 AA.
AC O02744; O46671;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 25-MAY-2022, entry version 128.
DE RecName: Full=Interleukin-12 subunit beta;
DE Short=IL-12B;
DE AltName: Full=Cytotoxic lymphocyte maturation factor 40 kDa subunit;
DE Short=CLMF p40;
DE AltName: Full=IL-12 subunit p40;
DE Flags: Precursor;
GN Name=IL12B;
OS Felis catus (Cat) (Felis silvestris catus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Feliformia; Felidae; Felinae; Felis.
OX NCBI_TaxID=9685;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Peripheral blood;
RX PubMed=9089110; DOI=10.1007/s002510050234;
RA Schijns V.E.C.J., Wierda C.M.H., Vahlenkamp T.W., Horzinek M.C.;
RT "Molecular cloning of cat interleukin-12.";
RL Immunogenetics 45:462-463(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9522125; DOI=10.3109/10425179709020889;
RA Fehr D., Dean G.A., Huder J., Fan Z., Huettner S., Higgins J.W.,
RA Pedersen N.C., Lutz H.;
RT "Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-
RT 12).";
RL DNA Seq. 8:77-82(1997).
CC -!- FUNCTION: Cytokine that can act as a growth factor for activated T and
CC NK cells, enhance the lytic activity of NK/lymphokine-activated killer
CC cells, and stimulate the production of IFN-gamma by resting PBMC.
CC {ECO:0000250}.
CC -!- FUNCTION: Associates with IL23A to form the IL-23 interleukin, a
CC heterodimeric cytokine which functions in innate and adaptive immunity.
CC IL-23 may constitute with IL-17 an acute response to infection in
CC peripheral tissues. IL-23 binds to a heterodimeric receptor complex
CC composed of IL12RB1 and IL23R, activates the Jak-Stat signaling
CC cascade, stimulates memory rather than naive T-cells and promotes
CC production of pro-inflammatory cytokines. IL-23 induces autoimmune
CC inflammation and thus may be responsible for autoimmune inflammatory
CC diseases and may be important for tumorigenesis (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer with IL12A; disulfide-linked. The heterodimer is
CC known as interleukin IL-12. Heterodimer with IL23A; disulfide-linked.
CC The heterodimer is known as interleukin IL-23. Also secreted as a
CC monomer (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the IL-12B family. {ECO:0000305}.
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DR EMBL; Y07762; CAA69068.1; -; mRNA.
DR EMBL; U83184; AAB93835.1; -; mRNA.
DR RefSeq; NP_001070881.1; NM_001077413.1.
DR AlphaFoldDB; O02744; -.
DR SMR; O02744; -.
DR STRING; 9685.ENSFCAP00000014439; -.
DR GeneID; 768273; -.
DR KEGG; fca:768273; -.
DR CTD; 3593; -.
DR eggNOG; ENOG502RZMA; Eukaryota.
DR InParanoid; O02744; -.
DR OrthoDB; 1179405at2759; -.
DR TreeFam; TF334829; -.
DR Proteomes; UP000011712; Unplaced.
DR GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR GO; GO:0043514; C:interleukin-12 complex; IBA:GO_Central.
DR GO; GO:0070743; C:interleukin-23 complex; IBA:GO_Central.
DR GO; GO:0043235; C:receptor complex; IBA:GO_Central.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0019955; F:cytokine binding; IBA:GO_Central.
DR GO; GO:0004896; F:cytokine receptor activity; IBA:GO_Central.
DR GO; GO:0042164; F:interleukin-12 alpha subunit binding; IBA:GO_Central.
DR GO; GO:0019221; P:cytokine-mediated signaling pathway; IBA:GO_Central.
DR CDD; cd00063; FN3; 1.
DR Gene3D; 2.60.40.10; -; 3.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR003530; Hematopoietin_rcpt_L_F3_CS.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003598; Ig_sub2.
DR InterPro; IPR015528; IL-12_beta.
DR InterPro; IPR019482; IL-12_beta_cen-dom.
DR PANTHER; PTHR23036:SF156; PTHR23036:SF156; 1.
DR Pfam; PF10420; IL12p40_C; 1.
DR PIRSF; PIRSF038007; IL_12_beta; 1.
DR PRINTS; PR01928; INTRLEUKN12B.
DR SMART; SM00408; IGc2; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR SUPFAM; SSF49265; SSF49265; 2.
DR PROSITE; PS50853; FN3; 1.
DR PROSITE; PS01354; HEMATOPO_REC_L_F3; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 2: Evidence at transcript level;
KW Cytokine; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000250"
FT CHAIN 23..329
FT /note="Interleukin-12 subunit beta"
FT /id="PRO_0000010928"
FT DOMAIN 23..106
FT /note="Ig-like C2-type"
FT DOMAIN 238..329
FT /note="Fibronectin type-III"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT CARBOHYD 135
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 223
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 50..90
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 200
FT /note="Interchain (with C-99 in IL12A and C-79 in IL23A)"
FT /evidence="ECO:0000250|UniProtKB:P29460,
FT ECO:0000255|PROSITE-ProRule:PRU00114"
FT CONFLICT 11
FT /note="L -> F (in Ref. 2; AAB93835)"
FT /evidence="ECO:0000305"
FT CONFLICT 51
FT /note="N -> D (in Ref. 2; AAB93835)"
FT /evidence="ECO:0000305"
FT CONFLICT 167
FT /note="E -> G (in Ref. 2; AAB93835)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 329 AA; 37374 MW; 12A069BC6322AE08 CRC64;
MHPQQLVIAW LSLVLLAPPL MAIWELEKNV YVVELDWHPD APGEMVVLTC NTPEEDDITW
TSDQSSEVLG SGKTLTIQVK EFADAGQYTC HKGGEVLSHS FLLIHKKEDG IWSTDILREQ
KESKNKIFLK CEAKNYSGRF TCWWLTAIST DLKFTVKSSR GSSDPQEVTC GAATLSAEKV
RVDNRDYKKY TVECQEGSAC PAAEESLPIE VVVDAIHKLK YENYTSSFFI RDIIKPDPPK
NLQLKPLKNS RHVEVSWEYP DTWSTPHSYF SLTFGVQVQG KNNREKKDRL SVDKTSAKVV
CHKDAKIRVQ ARDRYYSSSW SNWASVSCS
