ID   IL12B_HORSE             Reviewed;         329 AA.
AC   Q9XSQ5;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 122.
DE   RecName: Full=Interleukin-12 subunit beta;
DE            Short=IL-12B;
DE   AltName: Full=Cytotoxic lymphocyte maturation factor 40 kDa subunit;
DE            Short=CLMF p40;
DE   AltName: Full=IL-12 subunit p40;
DE   Flags: Precursor;
GN   Name=IL12B;
OS   Equus caballus (Horse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10541814; DOI=10.1007/s002510050693;
RA   Nicolson L., Penha-Goncalves M.N., Keanie J.L., Logan N.A., Argyle D.J.,
RA   Onions D.E.;
RT   "Cloning and sequencing of horse interleukin-12 and interleukin-18 cDNAs.";
RL   Immunogenetics 50:94-97(1999).
CC   -!- FUNCTION: Cytokine that can act as a growth factor for activated T and
CC       NK cells, enhance the lytic activity of NK/lymphokine-activated killer
CC       cells, and stimulate the production of IFN-gamma by resting PBMC.
CC       {ECO:0000250}.
CC   -!- FUNCTION: Associates with IL23A to form the IL-23 interleukin, a
CC       heterodimeric cytokine which functions in innate and adaptive immunity.
CC       IL-23 may constitute with IL-17 an acute response to infection in
CC       peripheral tissues. IL-23 binds to a heterodimeric receptor complex
CC       composed of IL12RB1 and IL23R, activates the Jak-Stat signaling
CC       cascade, stimulates memory rather than naive T-cells and promotes
CC       production of pro-inflammatory cytokines. IL-23 induces autoimmune
CC       inflammation and thus may be responsible for autoimmune inflammatory
CC       diseases and may be important for tumorigenesis (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer with IL12A; disulfide-linked. The heterodimer is
CC       known as interleukin IL-12. Heterodimer with IL23A; disulfide-linked.
CC       The heterodimer is known as interleukin IL-23. Also secreted as a
CC       monomer (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the IL-12B family. {ECO:0000305}.
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DR   EMBL; Y11129; CAA72011.1; -; mRNA.
DR   RefSeq; NP_001075985.1; NM_001082516.1.
DR   AlphaFoldDB; Q9XSQ5; -.
DR   SMR; Q9XSQ5; -.
DR   STRING; 9796.ENSECAP00000010803; -.
DR   PaxDb; Q9XSQ5; -.
DR   GeneID; 100034222; -.
DR   KEGG; ecb:100034222; -.
DR   CTD; 3593; -.
DR   InParanoid; Q9XSQ5; -.
DR   OrthoDB; 1179405at2759; -.
DR   Proteomes; UP000002281; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR   GO; GO:0004896; F:cytokine receptor activity; IEA:InterPro.
DR   CDD; cd00063; FN3; 1.
DR   Gene3D; 2.60.40.10; -; 3.
DR   InterPro; IPR003961; FN3_dom.
DR   InterPro; IPR036116; FN3_sf.
DR   InterPro; IPR003530; Hematopoietin_rcpt_L_F3_CS.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR003598; Ig_sub2.
DR   InterPro; IPR015528; IL-12_beta.
DR   InterPro; IPR019482; IL-12_beta_cen-dom.
DR   PANTHER; PTHR23036:SF156; PTHR23036:SF156; 1.
DR   Pfam; PF10420; IL12p40_C; 1.
DR   PIRSF; PIRSF038007; IL_12_beta; 1.
DR   PRINTS; PR01928; INTRLEUKN12B.
DR   SMART; SM00408; IGc2; 1.
DR   SUPFAM; SSF48726; SSF48726; 1.
DR   SUPFAM; SSF49265; SSF49265; 2.
DR   PROSITE; PS50853; FN3; 1.
DR   PROSITE; PS01354; HEMATOPO_REC_L_F3; 1.
DR   PROSITE; PS50835; IG_LIKE; 1.
PE   2: Evidence at transcript level;
KW   Cytokine; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000250"
FT   CHAIN           23..329
FT                   /note="Interleukin-12 subunit beta"
FT                   /id="PRO_0000010929"
FT   DOMAIN          29..106
FT                   /note="Ig-like C2-type"
FT   DOMAIN          238..329
FT                   /note="Fibronectin type-III"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT   CARBOHYD        125
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        135
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        223
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        50..90
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT   DISULFID        200
FT                   /note="Interchain (with C-99 in IL12A and C-76 in IL23A)"
FT                   /evidence="ECO:0000250|UniProtKB:P29460,
FT                   ECO:0000255|PROSITE-ProRule:PRU00114"
SQ   SEQUENCE   329 AA;  37442 MW;  7104BB0DFF8FA73C CRC64;
     MCHQWLVLSW FSLVLLASPL MAIWELEKDV YVVELDWYPD APGEMVVLTC NTPEEEGITW
     TSAQSNEVLG SGKTLTIQVK EFGDAGWYTC HKGGEVLSHS HLLLHKKEDG IWSTDILKDQ
     KESKNKTFLK CEAKNYSGRF TCWWLTAIST DLKFSVKSSR GSSDPRGVTC GAATLSAERV
     SVDDREYKKY TVECQEGSAC PAAEESLPIE IVVDAVHKLK YENYTSGFFI RDIIKPDPPK
     NLQLKPLKNS RQVEVSWEYP ETWSTPHSYF SLTFSIQVQG KNKKERKDRL FMDETSATVT
     CHKDGQIRVQ ARDRYYSSSW SEWASVSCS