IL1A_RABIT
ID IL1A_RABIT Reviewed; 267 AA.
AC P04822;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Interleukin-1 alpha;
DE Short=IL-1 alpha;
DE Flags: Precursor;
GN Name=IL1A;
OS Oryctolagus cuniculus (Rabbit).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus.
OX NCBI_TaxID=9986;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2994016; DOI=10.1093/nar/13.16.5869;
RA Furutani Y., Notake M., Yamayoshi M., Yamagishi J., Nomura H., Ohue M.,
RA Furuta R., Fukui T., Yamada M., Nakamura S.;
RT "Cloning and characterization of the cDNAs for human and rabbit
RT interleukin-1 precursor.";
RL Nucleic Acids Res. 13:5869-5882(1985).
CC -!- FUNCTION: Cytokine constitutively present intracellularly in nearly all
CC resting non-hematopoietic cells that plays an important role in
CC inflammation and bridges the innate and adaptive immune systems. After
CC binding to its receptor IL1R1 together with its accessory protein
CC IL1RAP, forms the high affinity interleukin-1 receptor complex.
CC Signaling involves the recruitment of adapter molecules such as MYD88,
CC IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the
CC three MAPK pathways p38, p42/p44 and JNK pathways. Within the cell,
CC acts as an alarmin and cell death results in its liberation in the
CC extracellular space after disruption of the cell membrane to induce
CC inflammation and alert the host to injury or damage. In addition to its
CC role as a danger signal, which occurs when the cytokine is passively
CC released by cell necrosis, directly senses DNA damage and acts as
CC signal for genotoxic stress without loss of cell integrity.
CC {ECO:0000250|UniProtKB:P01583}.
CC -!- SUBUNIT: Monomer. Interacts with TMED10; the interaction mediates the
CC translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-
CC Golgi intermediate compartment) and thereby secretion. Interacts with
CC IL1R1. Interacts with S100A13; this interaction is the first step in
CC the export of IL1A, followed by direct translocation of this complex
CC across the plasma membrane. {ECO:0000250|UniProtKB:P01583}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P01583}. Cytoplasm
CC {ECO:0000250|UniProtKB:P01583}. Secreted
CC {ECO:0000250|UniProtKB:P01583}. Note=The lack of a specific hydrophobic
CC segment in the precursor sequence suggests that IL-1 is released by
CC damaged cells or is secreted by a mechanism differing from that used
CC for other secretory proteins. The secretion is dependent on protein
CC unfolding and facilitated by the cargo receptor TMED10; it results in
CC protein translocation from the cytoplasm into the ERGIC (endoplasmic
CC reticulum-Golgi intermediate compartment) followed by vesicle entry and
CC secretion. Recruited to DNA damage sites and secreted after genotoxic
CC stress. {ECO:0000250|UniProtKB:P01583}.
CC -!- DOMAIN: The similarity among the IL-1 precursors suggests that the
CC amino ends of these proteins serve some as yet undefined function.
CC -!- PTM: Acetylated within its nuclear localization sequence, which impacts
CC subcellular localization. {ECO:0000250|UniProtKB:P01583}.
CC -!- PTM: Proteolytic processed by CAPN1 in a calcium-dependent manner.
CC Cleavage from 31 kDa precursor to 18 kDa biologically active molecules.
CC {ECO:0000250|UniProtKB:P01583}.
CC -!- PTM: Phosphorylated. Phosphorylation greatly enhances susceptibility to
CC digestion and promotes the conversion of pre-IL1A alpha to the
CC biologically active IL1A. {ECO:0000250|UniProtKB:P01583}.
CC -!- SIMILARITY: Belongs to the IL-1 family. {ECO:0000305}.
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DR EMBL; X02852; CAA26605.1; -; mRNA.
DR PIR; B24073; B24073.
DR RefSeq; NP_001095154.1; NM_001101684.1.
DR RefSeq; XP_017205589.1; XM_017350100.1.
DR AlphaFoldDB; P04822; -.
DR SMR; P04822; -.
DR STRING; 9986.ENSOCUP00000007573; -.
DR Ensembl; ENSOCUT00000008771; ENSOCUP00000007573; ENSOCUG00000008771.
DR GeneID; 100009250; -.
DR KEGG; ocu:100009250; -.
DR CTD; 3552; -.
DR eggNOG; ENOG502T3DD; Eukaryota.
DR GeneTree; ENSGT00390000013353; -.
DR HOGENOM; CLU_090014_0_0_1; -.
DR InParanoid; P04822; -.
DR OMA; SNMKYNF; -.
DR OrthoDB; 1513671at2759; -.
DR TreeFam; TF300203; -.
DR Proteomes; UP000001811; Chromosome 2.
DR Bgee; ENSOCUG00000008771; Expressed in testis and 5 other tissues.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR GO; GO:0005507; F:copper ion binding; ISS:UniProtKB.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0005149; F:interleukin-1 receptor binding; IEA:InterPro.
DR GO; GO:0034605; P:cellular response to heat; ISS:UniProtKB.
DR GO; GO:0006883; P:cellular sodium ion homeostasis; IEA:Ensembl.
DR GO; GO:0002248; P:connective tissue replacement involved in inflammatory response wound healing; IEA:Ensembl.
DR GO; GO:0019221; P:cytokine-mediated signaling pathway; IEA:Ensembl.
DR GO; GO:0035234; P:ectopic germ cell programmed cell death; IEA:Ensembl.
DR GO; GO:0097192; P:extrinsic apoptotic signaling pathway in absence of ligand; IEA:Ensembl.
DR GO; GO:0001660; P:fever generation; IEA:UniProtKB-KW.
DR GO; GO:0006955; P:immune response; IEA:InterPro.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IEA:Ensembl.
DR GO; GO:0045766; P:positive regulation of angiogenesis; IEA:Ensembl.
DR GO; GO:0051781; P:positive regulation of cell division; IEA:UniProtKB-KW.
DR GO; GO:0032743; P:positive regulation of interleukin-2 production; IEA:Ensembl.
DR GO; GO:0045840; P:positive regulation of mitotic nuclear division; IEA:Ensembl.
DR GO; GO:0050714; P:positive regulation of protein secretion; IEA:Ensembl.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR GO; GO:0010575; P:positive regulation of vascular endothelial growth factor production; IEA:Ensembl.
DR GO; GO:0046688; P:response to copper ion; ISS:UniProtKB.
DR InterPro; IPR003295; IL-1_alpha.
DR InterPro; IPR020877; IL-1_CS.
DR InterPro; IPR000975; IL-1_fam.
DR InterPro; IPR003502; IL-1_propep.
DR InterPro; IPR008996; IL1/FGF.
DR PANTHER; PTHR10078; PTHR10078; 1.
DR PANTHER; PTHR10078:SF33; PTHR10078:SF33; 1.
DR Pfam; PF00340; IL1; 1.
DR Pfam; PF02394; IL1_propep; 1.
DR PRINTS; PR00264; INTERLEUKIN1.
DR PRINTS; PR01358; INTRLEUKIN1A.
DR SUPFAM; SSF50353; SSF50353; 1.
DR PROSITE; PS00253; INTERLEUKIN_1; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Cytokine; Cytoplasm; Glycoprotein; Inflammatory response;
KW Mitogen; Nucleus; Phosphoprotein; Pyrogen; Reference proteome; Secreted.
FT PROPEP 1..112
FT /id="PRO_0000015277"
FT CHAIN 113..267
FT /note="Interleukin-1 alpha"
FT /id="PRO_0000015278"
FT REGION 82..86
FT /note="Nuclear localization signal (NLS)"
FT /evidence="ECO:0000250|UniProtKB:P01583"
FT MOD_RES 82
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P01583"
FT MOD_RES 87
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P01582"
FT CARBOHYD 64
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 100
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 141
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 267 AA; 30375 MW; 6D20533FD1FA4822 CRC64;
MAKVPDLFED LKNCFSENEE YSSAIDHLSL NQKSFYDASY EPLHEDCMNK VVSLSTSETS
VSPNLTFQEN VVAVTASGKI LKKRRLSLNQ PITDVDLETN VSDPEEGIIK PRSVPYTFQR
NMRYKYLRII KQEFTLNDAL NQSLVRDTSD QYLRAAPLQN LGDAVKFDMG VYMTSEDSIL
PVTLRISQTP LFVSAQNEDE PVLLKEMPET PRIITDSESD ILFFWETQGN KNYFKSAANP
QLFIATKPEH LVHMARGLPS MTDFQIS
