IL25_HUMAN
ID IL25_HUMAN Reviewed; 177 AA.
AC Q9H293; Q2M3F0; Q8IZV3; Q8WXB0;
DT 31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Interleukin-25;
DE Short=IL-25;
DE AltName: Full=Interleukin-17E;
DE Short=IL-17E;
DE Flags: Precursor;
GN Name=IL25; Synonyms=IL17E; ORFNames=UNQ3120/PRO10272;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=11058597; DOI=10.1074/jbc.m008289200;
RA Lee J., Ho W.-H., Maruoka M., Corpuz R.T., Baldwin D.T., Foster J.S.,
RA Goddard A.D., Yansura D.G., Vandlen R.L., Wood W.I., Gurney A.L.;
RT "IL-17E, a novel proinflammatory ligand for the IL-17 receptor homolog IL-
RT 17Rh1.";
RL J. Biol. Chem. 276:1660-1664(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=11754819; DOI=10.1016/s1074-7613(01)00243-6;
RA Fort M.M., Cheung J., Yen D., Li J., Zurawski S.M., Lo S., Menon S.,
RA Clifford T., Hunte B., Lesley R., Muchamuel T., Hurst S.D., Zurawski G.,
RA Leach M.W., Gorman D.M., Rennick D.M.;
RT "IL-25 induces IL-4, IL-5, and IL-13 and Th2-associated pathologies in
RT vivo.";
RL Immunity 15:985-995(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=12239140; DOI=10.1182/blood-2002-01-0012;
RA Kim M.R., Manoukian R., Yeh R., Silbiger S.M., Danilenko D.M., Scully S.,
RA Sun J., DeRose M.L., Stolina M., Chang D., Van G.Y., Clarkin K.,
RA Nguyen H.Q., Yu Y.B., Jing S., Senaldi G., Elliott G., Medlock E.S.;
RT "Transgenic overexpression of human IL-17E results in eosinophilia, B-
RT lymphocyte hyperplasia, and altered antibody production.";
RL Blood 100:2330-2340(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PROTEIN SEQUENCE OF 33-47.
RX PubMed=15340161; DOI=10.1110/ps.04682504;
RA Zhang Z., Henzel W.J.;
RT "Signal peptide prediction based on analysis of experimentally verified
RT cleavage sites.";
RL Protein Sci. 13:2819-2824(2004).
CC -!- FUNCTION: Induces activation of NF-kappa-B and stimulates production of
CC the pro-inflammatory chemokine IL-8. Pro-inflammatory cytokine favoring
CC Th2-type immune responses.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9H293-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9H293-2; Sequence=VSP_010159;
CC -!- TISSUE SPECIFICITY: Expressed at low levels in several tissues,
CC including brain, kidney, lung, prostate, testis, spinal cord, adrenal
CC gland, and trachea.
CC -!- SIMILARITY: Belongs to the IL-17 family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Wikipedia; Note=Interleukin-17 entry;
CC URL="https://en.wikipedia.org/wiki/Interleukin_17";
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DR EMBL; AF305200; AAG40848.1; -; mRNA.
DR EMBL; AF458059; AAL57622.1; -; mRNA.
DR EMBL; AF461739; AAN39038.1; -; mRNA.
DR EMBL; AY359127; AAQ89484.1; -; mRNA.
DR EMBL; CH471078; EAW66162.1; -; Genomic_DNA.
DR EMBL; BC069565; AAH69565.1; -; mRNA.
DR EMBL; BC104929; AAI04930.1; -; mRNA.
DR EMBL; BC104931; AAI04932.1; -; mRNA.
DR CCDS; CCDS45086.1; -. [Q9H293-2]
DR CCDS; CCDS9597.1; -. [Q9H293-1]
DR RefSeq; NP_073626.1; NM_022789.3. [Q9H293-1]
DR RefSeq; NP_758525.1; NM_172314.1. [Q9H293-2]
DR AlphaFoldDB; Q9H293; -.
DR SMR; Q9H293; -.
DR BioGRID; 122310; 50.
DR IntAct; Q9H293; 1.
DR STRING; 9606.ENSP00000328111; -.
DR GlyGen; Q9H293; 2 sites, 1 O-linked glycan (1 site).
DR iPTMnet; Q9H293; -.
DR PhosphoSitePlus; Q9H293; -.
DR BioMuta; IL25; -.
DR DMDM; 20138730; -.
DR MassIVE; Q9H293; -.
DR PaxDb; Q9H293; -.
DR PeptideAtlas; Q9H293; -.
DR PRIDE; Q9H293; -.
DR ProteomicsDB; 80511; -. [Q9H293-1]
DR ProteomicsDB; 80512; -. [Q9H293-2]
DR TopDownProteomics; Q9H293-2; -. [Q9H293-2]
DR ABCD; Q9H293; 28 sequenced antibodies.
DR Antibodypedia; 22458; 677 antibodies from 38 providers.
DR DNASU; 64806; -.
DR Ensembl; ENST00000329715.2; ENSP00000328111.2; ENSG00000166090.8. [Q9H293-1]
DR Ensembl; ENST00000397242.2; ENSP00000380417.2; ENSG00000166090.8. [Q9H293-2]
DR GeneID; 64806; -.
DR KEGG; hsa:64806; -.
DR UCSC; uc001wjq.4; human. [Q9H293-1]
DR CTD; 64806; -.
DR DisGeNET; 64806; -.
DR GeneCards; IL25; -.
DR HGNC; HGNC:13765; IL25.
DR HPA; ENSG00000166090; Not detected.
DR MIM; 605658; gene.
DR neXtProt; NX_Q9H293; -.
DR OpenTargets; ENSG00000166090; -.
DR PharmGKB; PA29799; -.
DR VEuPathDB; HostDB:ENSG00000166090; -.
DR eggNOG; ENOG502S755; Eukaryota.
DR GeneTree; ENSGT00930000151068; -.
DR HOGENOM; CLU_139236_0_0_1; -.
DR InParanoid; Q9H293; -.
DR OMA; HHGYCLE; -.
DR OrthoDB; 1298853at2759; -.
DR PhylomeDB; Q9H293; -.
DR TreeFam; TF314701; -.
DR PathwayCommons; Q9H293; -.
DR Reactome; R-HSA-448424; Interleukin-17 signaling.
DR SignaLink; Q9H293; -.
DR BioGRID-ORCS; 64806; 11 hits in 1064 CRISPR screens.
DR GeneWiki; Interleukin_25; -.
DR GenomeRNAi; 64806; -.
DR Pharos; Q9H293; Tbio.
DR PRO; PR:Q9H293; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; Q9H293; protein.
DR Bgee; ENSG00000166090; Expressed in right atrium auricular region and 39 other tissues.
DR Genevisible; Q9H293; HS.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0030380; F:interleukin-17E receptor binding; TAS:UniProtKB.
DR GO; GO:0030222; P:eosinophil differentiation; IEA:Ensembl.
DR GO; GO:0002437; P:inflammatory response to antigenic stimulus; IEA:Ensembl.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR GO; GO:0009620; P:response to fungus; IEA:Ensembl.
DR GO; GO:0009624; P:response to nematode; IEA:Ensembl.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR010345; IL-17_fam.
DR Pfam; PF06083; IL17; 1.
DR SUPFAM; SSF57501; SSF57501; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytokine; Direct protein sequencing; Disulfide bond;
KW Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..32
FT /evidence="ECO:0000269|PubMed:15340161"
FT CHAIN 33..177
FT /note="Interleukin-25"
FT /id="PRO_0000015431"
FT REGION 58..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 136
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 110..168
FT /evidence="ECO:0000250"
FT DISULFID 115..170
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..18
FT /note="MRERPRLGEDSSLISLFL -> MY (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11754819,
FT ECO:0000303|PubMed:12239140, ECO:0000303|PubMed:15489334"
FT /id="VSP_010159"
FT CONFLICT 177
FT /note="G -> A (in Ref. 2; AAL57622)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 177 AA; 20330 MW; 52D895710CD59871 CRC64;
MRERPRLGED SSLISLFLQV VAFLAMVMGT HTYSHWPSCC PSKGQDTSEE LLRWSTVPVP
PLEPARPNRH PESCRASEDG PLNSRAISPW RYELDRDLNR LPQDLYHARC LCPHCVSLQT
GSHMDPRGNS ELLYHNQTVF YRRPCHGEKG THKGYCLERR LYRVSLACVC VRPRVMG
