IL27A_PIG
ID IL27A_PIG Reviewed; 239 AA.
AC Q5S1V9;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 25-MAY-2022, entry version 54.
DE RecName: Full=Interleukin-27 subunit alpha;
DE Short=IL-27 subunit alpha;
DE Short=IL-27-A;
DE Short=IL27-A;
DE AltName: Full=p28;
DE Flags: Precursor;
GN Name=IL27; Synonyms=IL27A;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Peng H.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Associates with EBI3 to form the IL-27 interleukin, a
CC heterodimeric cytokine which functions in innate immunity. Cytokine
CC with pro- and anti-inflammatory properties, that can regulate T-helper
CC cell development, suppress T-cell proliferation, stimulate cytotoxic T-
CC cell activity, induce isotype switching in B-cells, and that has
CC diverse effects on innate immune cells. Among its target cells are CD4
CC T-helper cells which can differentiate in type 1 effector cells (TH1),
CC type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17).
CC It drives rapid clonal expansion of naive but not memory CD4 T-cells.
CC It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-
CC gamma production of naive CD4 T-cells, binds to the cytokine receptor
CC WSX-1/TCCR which appears to be required but not sufficient for IL-27-
CC mediated signal transduction. IL-27 potentiate the early phase of TH1
CC response and suppress TH2 and TH17 differentiation. It induces the
CC differentiation of TH1 cells via two distinct pathways, p38
CC MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces
CC STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet
CC via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1
CC cell commitment. It suppresses the expression of GATA3, the inhibitor
CC TH1 cells development. In CD8 T-cells, it activates STATs as well as
CC GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development
CC and of IL-17 production. Indeed IL27 alone is also able to inhibit the
CC production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the
CC development of pro-inflammatory Th17 cells via STAT1, it inhibits the
CC development of anti-inflammatory inducible regulatory T-cells, iTreg,
CC independently of STAT1. IL-27 has also an effect on cytokine
CC production, it suppresses pro-inflammatory cytokine production such as
CC IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling
CC such as SOCS1 and SOCS3. Apart from suppression of cytokine production,
CC IL-27 also antagonizes the effects of some cytokines such as IL6
CC through direct effects on T-cells. Another important role of IL-27 is
CC its antitumor activity as well as its antiangiogenic activity with
CC activation of production of antiangiogenic chemokines such as IP-
CC 10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces
CC IRF1/interferon regulatory factor 1 and increase the expression of MHC
CC class II transactivator/CIITA with resulting up-regulation of major
CC histocompatibility complex class II (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer with EBI3; not disulfide-linked. This heterodimer
CC is known as interleukin IL-27 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Note=Does not seem to be
CC secreted without coexpression of EBI3. {ECO:0000250}.
CC -!- PTM: O-glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the IL-6 superfamily. {ECO:0000305}.
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DR EMBL; AY788913; AAV54185.1; -; mRNA.
DR RefSeq; NP_001007521.1; NM_001007520.1.
DR AlphaFoldDB; Q5S1V9; -.
DR SMR; Q5S1V9; -.
DR GeneID; 493187; -.
DR KEGG; ssc:493187; -.
DR CTD; 246778; -.
DR InParanoid; Q5S1V9; -.
DR OrthoDB; 1356544at2759; -.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Unplaced.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0045523; F:interleukin-27 receptor binding; IEA:InterPro.
DR GO; GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0042129; P:regulation of T cell proliferation; IEA:InterPro.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR026207; IL-27_alpha.
DR PANTHER; PTHR20879; PTHR20879; 1.
PE 2: Evidence at transcript level;
KW Cytokine; Glycoprotein; Immunity; Inflammatory response; Innate immunity;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..239
FT /note="Interleukin-27 subunit alpha"
FT /id="PRO_0000320141"
FT REGION 167..186
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 239 AA; 26643 MW; BC3870C56697AB1B CRC64;
MGQMADDLGW RLSLLLLSLL LARAGVWGFP RPPGRPPLSL QELQREFKVS LHLARKLLSE
VRVQARHFAE SHLPGVNLDL LPLGEQLPNV SLNFQAWRGL SDPERLCFLS MTLRPFHTLL
GGLGSQGFWT SSERMQLWAI RLDLRDLQQH LRFQVLAAGF NLPGQEEEEN EAGRELLPGA
PGGPSKPAAQ VSWPRLLYTY QLLHSLELVL SRAMRDFLLL SRAGNPAPAL GFPTPSSPP
