IL27B_MOUSE
ID IL27B_MOUSE Reviewed; 228 AA.
AC O35228;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Interleukin-27 subunit beta;
DE Short=IL-27 subunit beta;
DE Short=IL-27B;
DE AltName: Full=Epstein-Barr virus-induced gene 3 protein homolog;
DE Flags: Precursor;
GN Name=Ebi3; Synonyms=Il27b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Embryo;
RA Nomura H., Yaguchi N., Hanyuu C., Maeda M., Kikuchi Y., Nakata Y.,
RA Kojima T., Tulin E.E., Hasegawa M.;
RT "Mouse homolog of human Epstein-Barr virus-induced gene 3 (EBI3).";
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP REVIEW.
RX PubMed=17294231; DOI=10.1007/s00109-007-0164-7;
RA Batten M., Ghilardi N.;
RT "The biology and therapeutic potential of interleukin 27.";
RL J. Mol. Med. 85:661-672(2007).
CC -!- FUNCTION: Associates with IL27 to form the IL-27 interleukin, a
CC heterodimeric cytokine which functions in innate immunity. IL-27 has
CC pro- and anti-inflammatory properties, that can regulate T-helper cell
CC development, suppress T-cell proliferation, stimulate cytotoxic T-cell
CC activity, induce isotype switching in B-cells, and that has diverse
CC effects on innate immune cells. Among its target cells are CD4 T-helper
CC cells which can differentiate in type 1 effector cells (TH1), type 2
CC effector cells (TH2) and IL17 producing helper T-cells (TH17). It
CC drives rapid clonal expansion of naive but not memory CD4 T-cells. It
CC also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-
CC gamma production of naive CD4 T-cells, binds to the cytokine receptor
CC WSX-1/TCCR. Another important role of IL-27 is its antitumor activity
CC as well as its antiangiogenic activity with activation of production of
CC antiangiogenic chemokines.
CC -!- SUBUNIT: Heterodimer with IL27/IL27A; not disulfide-linked. This
CC heterodimer is known as interleukin IL-27. Heterodimer with IL12A; not
CC disulfide-linked. This heterodimer is known as interleukin IL-35.
CC Interacts with SQSTM1. {ECO:0000250|UniProtKB:Q14213}.
CC -!- INTERACTION:
CC O35228; P43431: Il12a; NbExp=2; IntAct=EBI-3862967, EBI-3862959;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q14213}.
CC -!- SIMILARITY: Belongs to the type I cytokine receptor family. Type 3
CC subfamily. {ECO:0000305}.
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DR EMBL; AF013114; AAB67115.1; -; mRNA.
DR EMBL; BC008209; AAH08209.1; -; mRNA.
DR CCDS; CCDS28888.1; -.
DR RefSeq; NP_056581.1; NM_015766.2.
DR AlphaFoldDB; O35228; -.
DR SMR; O35228; -.
DR BioGRID; 206044; 1.
DR DIP; DIP-60364N; -.
DR IntAct; O35228; 1.
DR STRING; 10090.ENSMUSP00000003274; -.
DR GlyGen; O35228; 2 sites.
DR PaxDb; O35228; -.
DR PeptideAtlas; O35228; -.
DR PRIDE; O35228; -.
DR ProteomicsDB; 269393; -.
DR Antibodypedia; 23527; 1092 antibodies from 39 providers.
DR DNASU; 50498; -.
DR Ensembl; ENSMUST00000003274; ENSMUSP00000003274; ENSMUSG00000003206.
DR GeneID; 50498; -.
DR KEGG; mmu:50498; -.
DR UCSC; uc008daj.1; mouse.
DR CTD; 10148; -.
DR MGI; MGI:1354171; Ebi3.
DR VEuPathDB; HostDB:ENSMUSG00000003206; -.
DR eggNOG; ENOG502RXJ4; Eukaryota.
DR GeneTree; ENSGT00940000160050; -.
DR HOGENOM; CLU_047259_2_0_1; -.
DR InParanoid; O35228; -.
DR OMA; TSCTIAD; -.
DR OrthoDB; 857138at2759; -.
DR PhylomeDB; O35228; -.
DR TreeFam; TF331210; -.
DR BioGRID-ORCS; 50498; 3 hits in 72 CRISPR screens.
DR PRO; PR:O35228; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; O35228; protein.
DR Bgee; ENSMUSG00000003206; Expressed in granulocyte and 112 other tissues.
DR ExpressionAtlas; O35228; baseline and differential.
DR Genevisible; O35228; MM.
DR GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR GO; GO:0043235; C:receptor complex; IBA:GO_Central.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0019955; F:cytokine binding; IBA:GO_Central.
DR GO; GO:0004896; F:cytokine receptor activity; ISO:MGI.
DR GO; GO:0045523; F:interleukin-27 receptor binding; IDA:MGI.
DR GO; GO:0017046; F:peptide hormone binding; IBA:GO_Central.
DR GO; GO:0019221; P:cytokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0033210; P:leptin-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0042098; P:T cell proliferation; IDA:CACAO.
DR CDD; cd00063; FN3; 1.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR003530; Hematopoietin_rcpt_L_F3_CS.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR015373; Interferon/interleukin_rcp_dom.
DR Pfam; PF09294; Interfer-bind; 1.
DR SMART; SM00060; FN3; 1.
DR SUPFAM; SSF49265; SSF49265; 2.
DR PROSITE; PS50853; FN3; 2.
DR PROSITE; PS01354; HEMATOPO_REC_L_F3; 1.
PE 1: Evidence at protein level;
KW Cytokine; Glycoprotein; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..228
FT /note="Interleukin-27 subunit beta"
FT /id="PRO_0000010938"
FT DOMAIN 26..124
FT /note="Fibronectin type-III 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 127..224
FT /note="Fibronectin type-III 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT CARBOHYD 54
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 104
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 228 AA; 25353 MW; 49DEFA4E5C4F2126 CRC64;
MSKLLFLSLA LWASRSPGYT ETALVALSQP RVQCHASRYP VAVDCSWTPL QAPNSTRSTS
FIATYRLGVA TQQQSQPCLQ RSPQASRCTI PDVHLFSTVP YMLNVTAVHP GGASSSLLAF
VAERIIKPDP PEGVRLRTAG QRLQVLWHPP ASWPFPDIFS LKYRLRYRRR GASHFRQVGP
IEATTFTLRN SKPHAKYCIQ VSAQDLTDYG KPSDWSLPGQ VESAPHKP
