IL32_HUMAN
ID IL32_HUMAN Reviewed; 234 AA.
AC P24001; A6NNM0; A8MPX0; B4DJM1; B8Q191; D3DUB0; D3DUB2; Q5VFH7; Q5VFH8;
AC Q8WV38; Q96GK9;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 12-DEC-2006, sequence version 3.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Interleukin-32;
DE Short=IL-32;
DE AltName: Full=Natural killer cells protein 4;
DE AltName: Full=Tumor necrosis factor alpha-inducing factor;
DE Flags: Precursor;
GN Name=IL32; Synonyms=NK4, TAIF;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION, AND VARIANT ASN-152.
RX PubMed=1729377;
RA Dahl C.A., Schall R.P., He H., Cairns J.S.;
RT "Identification of a novel gene expressed in activated natural killer cells
RT and T cells.";
RL J. Immunol. 148:597-603(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 2; 3 AND 4), ALTERNATIVE
RP SPLICING, FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=15664165; DOI=10.1016/j.immuni.2004.12.003;
RA Kim S.-H., Han S.-Y., Azam T., Yoon D.-Y., Dinarello C.A.;
RT "Interleukin-32: a cytokine and inducer of TNFalpha.";
RL Immunity 22:131-142(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 7).
RA Mao W., Han W., Salama A., Moldenhauer A.;
RT "A new variant of interleukin 32.";
RL Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
RC TISSUE=Thalamus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 6).
RC TISSUE=Stomach;
RX PubMed=16341674; DOI=10.1007/s00335-005-0075-2;
RA Oh J.H., Yang J.O., Hahn Y., Kim M.R., Byun S.S., Jeon Y.J., Kim J.M.,
RA Song K.S., Noh S.M., Kim S., Yoo H.S., Kim Y.S., Kim N.S.;
RT "Transcriptome analysis of human gastric cancer.";
RL Mamm. Genome 16:942-954(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15616553; DOI=10.1038/nature03187;
RA Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G.,
RA Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E.,
RA Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., Buckingham J.M.,
RA Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C.,
RA Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M.,
RA Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M.,
RA Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D.,
RA Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L.,
RA Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E.,
RA Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H.,
RA Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y.,
RA Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S.,
RA Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A.,
RA Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M.,
RA Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H.,
RA Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A.,
RA Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J.,
RA DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J.,
RA Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M.,
RA Myers R.M., Rubin E.M., Pennacchio L.A.;
RT "The sequence and analysis of duplication-rich human chromosome 16.";
RL Nature 432:988-994(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Eye, and Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Cytokine that may play a role in innate and adaptive immune
CC responses. It induces various cytokines such as TNFA/TNF-alpha and IL8.
CC It activates typical cytokine signal pathways of NF-kappa-B and p38
CC MAPK. {ECO:0000269|PubMed:15664165}.
CC -!- INTERACTION:
CC P24001; Q6NTF9-3: RHBDD2; NbExp=3; IntAct=EBI-2866752, EBI-17589229;
CC P24001-2; P49715: CEBPA; NbExp=7; IntAct=EBI-8800907, EBI-1172054;
CC P24001-2; Q05655: PRKCD; NbExp=7; IntAct=EBI-8800907, EBI-704279;
CC P24001-3; P24001-2: IL32; NbExp=2; IntAct=EBI-8800897, EBI-8800907;
CC P24001-4; P24158: PRTN3; NbExp=3; IntAct=EBI-15570379, EBI-465028;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15664165}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=7;
CC Name=1; Synonyms=Gamma;
CC IsoId=P24001-1; Sequence=Displayed;
CC Name=2; Synonyms=Beta;
CC IsoId=P24001-2; Sequence=VSP_022006;
CC Name=3; Synonyms=Delta;
CC IsoId=P24001-3; Sequence=VSP_022005, VSP_022006;
CC Name=4; Synonyms=Alpha;
CC IsoId=P24001-4; Sequence=VSP_022006, VSP_022007;
CC Name=5;
CC IsoId=P24001-5; Sequence=VSP_022006, VSP_043318;
CC Name=6;
CC IsoId=P24001-6; Sequence=VSP_022006, VSP_045785;
CC Name=7;
CC IsoId=P24001-7; Sequence=VSP_047855, VSP_047856;
CC -!- TISSUE SPECIFICITY: Selectively expressed in lymphocytes. Expression is
CC more prominent in immune cells than in non-immune cells.
CC {ECO:0000269|PubMed:15664165}.
CC -!- INDUCTION: Expression increased after activation of T-cells by mitogens
CC or activation of NK cells by IL2/interleukin-2.
CC {ECO:0000269|PubMed:1729377}.
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DR EMBL; M59807; AAA36367.2; -; mRNA.
DR EMBL; AY495331; AAS80143.1; -; mRNA.
DR EMBL; AY495332; AAS80144.1; -; mRNA.
DR EMBL; AY495333; AAS80145.1; -; mRNA.
DR EMBL; AY495334; AAS80146.1; -; Genomic_DNA.
DR EMBL; AY495334; AAS80147.1; -; Genomic_DNA.
DR EMBL; AY495334; AAS80148.1; -; Genomic_DNA.
DR EMBL; EU339107; ACA50721.1; -; mRNA.
DR EMBL; AK296143; BAG58883.1; -; mRNA.
DR EMBL; BM825040; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AC108134; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471112; EAW85405.1; -; Genomic_DNA.
DR EMBL; CH471112; EAW85406.1; -; Genomic_DNA.
DR EMBL; CH471112; EAW85407.1; -; Genomic_DNA.
DR EMBL; CH471112; EAW85408.1; -; Genomic_DNA.
DR EMBL; CH471112; EAW85409.1; -; Genomic_DNA.
DR EMBL; CH471112; EAW85410.1; -; Genomic_DNA.
DR EMBL; BC009401; AAH09401.1; -; mRNA.
DR EMBL; BC018782; AAH18782.1; -; mRNA.
DR EMBL; BC105602; AAI05603.1; -; mRNA.
DR CCDS; CCDS32377.1; -. [P24001-2]
DR CCDS; CCDS32378.1; -. [P24001-4]
DR CCDS; CCDS32379.1; -. [P24001-6]
DR CCDS; CCDS45394.1; -. [P24001-5]
DR CCDS; CCDS76811.1; -. [P24001-1]
DR PIR; I56140; I56140.
DR RefSeq; NP_001012649.1; NM_001012631.1. [P24001-2]
DR RefSeq; NP_001012650.1; NM_001012632.1. [P24001-2]
DR RefSeq; NP_001012651.1; NM_001012633.1. [P24001-4]
DR RefSeq; NP_001012652.1; NM_001012634.1. [P24001-5]
DR RefSeq; NP_001012653.1; NM_001012635.1. [P24001-5]
DR RefSeq; NP_001012654.1; NM_001012636.1. [P24001-6]
DR RefSeq; NP_001012736.1; NM_001012718.1. [P24001-2]
DR RefSeq; NP_001295007.1; NM_001308078.1. [P24001-1]
DR RefSeq; NP_004212.4; NM_004221.4. [P24001-2]
DR AlphaFoldDB; P24001; -.
DR BioGRID; 114664; 35.
DR DIP; DIP-61119N; -.
DR IntAct; P24001; 6.
DR MINT; P24001; -.
DR iPTMnet; P24001; -.
DR PhosphoSitePlus; P24001; -.
DR SwissPalm; P24001; -.
DR BioMuta; IL32; -.
DR DMDM; 119364597; -.
DR EPD; P24001; -.
DR jPOST; P24001; -.
DR MassIVE; P24001; -.
DR MaxQB; P24001; -.
DR PeptideAtlas; P24001; -.
DR PRIDE; P24001; -.
DR ProteomicsDB; 1618; -.
DR ProteomicsDB; 54173; -. [P24001-1]
DR ProteomicsDB; 54174; -. [P24001-2]
DR ProteomicsDB; 54175; -. [P24001-3]
DR ProteomicsDB; 54176; -. [P24001-4]
DR ProteomicsDB; 54177; -. [P24001-5]
DR ProteomicsDB; 7283; -.
DR Antibodypedia; 10701; 590 antibodies from 40 providers.
DR DNASU; 9235; -.
DR Ensembl; ENST00000008180.13; ENSP00000008180.9; ENSG00000008517.19. [P24001-5]
DR Ensembl; ENST00000325568.9; ENSP00000324742.5; ENSG00000008517.19. [P24001-2]
DR Ensembl; ENST00000382213.7; ENSP00000371648.3; ENSG00000008517.19. [P24001-6]
DR Ensembl; ENST00000396887.7; ENSP00000380096.3; ENSG00000008517.19. [P24001-4]
DR Ensembl; ENST00000396890.6; ENSP00000380099.2; ENSG00000008517.19. [P24001-1]
DR Ensembl; ENST00000440815.7; ENSP00000405063.3; ENSG00000008517.19. [P24001-2]
DR Ensembl; ENST00000444393.7; ENSP00000411958.3; ENSG00000008517.19. [P24001-2]
DR Ensembl; ENST00000525643.7; ENSP00000432218.3; ENSG00000008517.19. [P24001-2]
DR Ensembl; ENST00000526464.6; ENSP00000450364.1; ENSG00000008517.19. [P24001-2]
DR Ensembl; ENST00000528163.6; ENSP00000432850.2; ENSG00000008517.19. [P24001-2]
DR Ensembl; ENST00000529550.5; ENSP00000437020.1; ENSG00000008517.19. [P24001-2]
DR Ensembl; ENST00000530538.6; ENSP00000436929.2; ENSG00000008517.19. [P24001-2]
DR Ensembl; ENST00000530890.5; ENSP00000433747.1; ENSG00000008517.19. [P24001-5]
DR Ensembl; ENST00000531965.5; ENSP00000433177.1; ENSG00000008517.19. [P24001-3]
DR Ensembl; ENST00000533097.6; ENSP00000432917.2; ENSG00000008517.19. [P24001-2]
DR Ensembl; ENST00000534507.5; ENSP00000431775.1; ENSG00000008517.19. [P24001-1]
DR Ensembl; ENST00000548246.1; ENSP00000447979.1; ENSG00000008517.19. [P24001-7]
DR Ensembl; ENST00000548476.5; ENSP00000449483.1; ENSG00000008517.19. [P24001-1]
DR Ensembl; ENST00000548652.5; ENSP00000446624.1; ENSG00000008517.19. [P24001-6]
DR Ensembl; ENST00000549213.5; ENSP00000447812.1; ENSG00000008517.19. [P24001-4]
DR Ensembl; ENST00000551122.5; ENSP00000447496.1; ENSG00000008517.19. [P24001-4]
DR Ensembl; ENST00000552356.5; ENSP00000446978.1; ENSG00000008517.19. [P24001-5]
DR Ensembl; ENST00000552664.5; ENSP00000448683.1; ENSG00000008517.19. [P24001-2]
DR GeneID; 9235; -.
DR KEGG; hsa:9235; -.
DR MANE-Select; ENST00000525643.7; ENSP00000432218.3; NM_001376923.1; NP_001363852.1. [P24001-2]
DR UCSC; uc002ctk.4; human. [P24001-1]
DR CTD; 9235; -.
DR DisGeNET; 9235; -.
DR GeneCards; IL32; -.
DR HGNC; HGNC:16830; IL32.
DR HPA; ENSG00000008517; Tissue enhanced (liver, pancreas).
DR MIM; 606001; gene.
DR neXtProt; NX_P24001; -.
DR OpenTargets; ENSG00000008517; -.
DR PharmGKB; PA128395754; -.
DR VEuPathDB; HostDB:ENSG00000008517; -.
DR GeneTree; ENSGT00940000167201; -.
DR InParanoid; P24001; -.
DR OMA; CCAVETV; -.
DR OrthoDB; 1509659at2759; -.
DR PhylomeDB; P24001; -.
DR TreeFam; TF341753; -.
DR PathwayCommons; P24001; -.
DR Reactome; R-HSA-449836; Other interleukin signaling.
DR Reactome; R-HSA-9013423; RAC3 GTPase cycle.
DR SignaLink; P24001; -.
DR SIGNOR; P24001; -.
DR BioGRID-ORCS; 9235; 12 hits in 1072 CRISPR screens.
DR ChiTaRS; IL32; human.
DR GenomeRNAi; 9235; -.
DR Pharos; P24001; Tbio.
DR PRO; PR:P24001; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; P24001; protein.
DR Bgee; ENSG00000008517; Expressed in granulocyte and 139 other tissues.
DR ExpressionAtlas; P24001; baseline and differential.
DR Genevisible; P24001; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005615; C:extracellular space; TAS:ProtInc.
DR GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0007155; P:cell adhesion; TAS:ProtInc.
DR GO; GO:0006952; P:defense response; TAS:ProtInc.
DR GO; GO:0006955; P:immune response; TAS:ProtInc.
DR GO; GO:1903901; P:negative regulation of viral life cycle; IDA:ARUK-UCL.
DR GO; GO:0010628; P:positive regulation of gene expression; IDA:ARUK-UCL.
DR GO; GO:1905636; P:positive regulation of RNA polymerase II regulatory region sequence-specific DNA binding; IDA:ARUK-UCL.
DR GO; GO:0034346; P:positive regulation of type III interferon production; IDA:ARUK-UCL.
DR InterPro; IPR028067; IL-32.
DR Pfam; PF15225; IL32; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytokine; Reference proteome; Secreted; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..234
FT /note="Interleukin-32"
FT /id="PRO_0000021487"
FT REGION 213..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..10
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15664165"
FT /id="VSP_022005"
FT VAR_SEQ 19..84
FT /note="Missing (in isoform 7)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_047855"
FT VAR_SEQ 19..64
FT /note="Missing (in isoform 2, isoform 3, isoform 4, isoform
FT 5 and isoform 6)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:15664165,
FT ECO:0000303|PubMed:16341674"
FT /id="VSP_022006"
FT VAR_SEQ 65..84
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_043318"
FT VAR_SEQ 85..93
FT /note="Missing (in isoform 6)"
FT /evidence="ECO:0000303|PubMed:16341674"
FT /id="VSP_045785"
FT VAR_SEQ 94..113
FT /note="Missing (in isoform 7)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_047856"
FT VAR_SEQ 154..210
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:15664165"
FT /id="VSP_022007"
FT VARIANT 152
FT /note="D -> N"
FT /evidence="ECO:0000269|PubMed:1729377"
FT /id="VAR_005506"
FT CONFLICT 233
FT /note="S -> L (in Ref. 5; BM825040)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 234 AA; 26676 MW; 5F339FE2D348A761 CRC64;
MCFPKVLSDD MKKLKARMVM LLPTSAQGLG AWVSACDTED TVGHLGPWRD KDPALWCQLC
LSSQHQAIER FYDKMQNAES GRGQVMSSLA ELEDDFKEGY LETVAAYYEE QHPELTPLLE
KERDGLRCRG NRSPVPDVED PATEEPGESF CDKVMRWFQA MLQRLQTWWH GVLAWVKEKV
VALVHAVQAL WKQFQSFCCS LSELFMSSFQ SYGAPRGDKE ELTPQKCSEP QSSK
