IL34_MOUSE
ID IL34_MOUSE Reviewed; 235 AA.
AC Q8R1R4; B2ZC71; B2ZC72; Q9D8F2;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Interleukin-34;
DE Short=IL-34;
DE Flags: Precursor;
GN Name=Il34;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=C57BL/6J;
RX PubMed=18467591; DOI=10.1126/science.1154370;
RA Lin H., Lee E., Hestir K., Leo C., Huang M., Bosch E., Halenbeck R., Wu G.,
RA Zhou A., Behrens D., Hollenbaugh D., Linnemann T., Qin M., Wong J., Chu K.,
RA Doberstein S.K., Williams L.T.;
RT "Discovery of a cytokine and its receptor by functional screening of the
RT extracellular proteome.";
RL Science 320:807-811(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Small intestine;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Cytokine that promotes the proliferation, survival and
CC differentiation of monocytes and macrophages. Promotes the release of
CC pro-inflammatory chemokines, and thereby plays an important role in
CC innate immunity and in inflammatory processes. Plays an important role
CC in the regulation of osteoclast proliferation and differentiation, and
CC in the regulation of bone resorption. Signaling via CSF1R and its
CC downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND
CC MAPK3/ERK1 (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Interacts with CSF1R (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8R1R4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8R1R4-2; Sequence=VSP_026632;
CC Name=3;
CC IsoId=Q8R1R4-3; Sequence=VSP_035080;
CC -!- SIMILARITY: Belongs to the IL-34 family. {ECO:0000305}.
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DR EMBL; EU636995; ACD13453.1; -; mRNA.
DR EMBL; EU636996; ACD13454.1; -; mRNA.
DR EMBL; AK008082; BAB25448.1; -; mRNA.
DR EMBL; BC016254; AAH16254.1; -; mRNA.
DR CCDS; CCDS52669.1; -. [Q8R1R4-1]
DR CCDS; CCDS52670.1; -. [Q8R1R4-2]
DR RefSeq; NP_001128572.1; NM_001135100.2. [Q8R1R4-1]
DR RefSeq; NP_083922.1; NM_029646.3. [Q8R1R4-2]
DR RefSeq; XP_006531548.1; XM_006531485.3. [Q8R1R4-3]
DR PDB; 4EXN; X-ray; 2.70 A; A/B/E/F=21-194.
DR PDB; 4EXP; X-ray; 2.80 A; A=21-194.
DR PDBsum; 4EXN; -.
DR PDBsum; 4EXP; -.
DR AlphaFoldDB; Q8R1R4; -.
DR SMR; Q8R1R4; -.
DR BioGRID; 218168; 2.
DR STRING; 10090.ENSMUSP00000076120; -.
DR GlyGen; Q8R1R4; 1 site.
DR PaxDb; Q8R1R4; -.
DR PRIDE; Q8R1R4; -.
DR ProteomicsDB; 269395; -. [Q8R1R4-1]
DR ProteomicsDB; 269396; -. [Q8R1R4-2]
DR ProteomicsDB; 269397; -. [Q8R1R4-3]
DR ABCD; Q8R1R4; 1 sequenced antibody.
DR Antibodypedia; 30010; 392 antibodies from 32 providers.
DR Ensembl; ENSMUST00000076846; ENSMUSP00000076120; ENSMUSG00000031750. [Q8R1R4-1]
DR Ensembl; ENSMUST00000150680; ENSMUSP00000114398; ENSMUSG00000031750. [Q8R1R4-2]
DR GeneID; 76527; -.
DR KEGG; mmu:76527; -.
DR UCSC; uc009nkz.3; mouse. [Q8R1R4-2]
DR UCSC; uc009nla.3; mouse. [Q8R1R4-1]
DR UCSC; uc012gko.1; mouse. [Q8R1R4-3]
DR CTD; 146433; -.
DR MGI; MGI:1923777; Il34.
DR VEuPathDB; HostDB:ENSMUSG00000031750; -.
DR eggNOG; ENOG502S2ET; Eukaryota.
DR GeneTree; ENSGT00390000000932; -.
DR HOGENOM; CLU_102223_1_0_1; -.
DR InParanoid; Q8R1R4; -.
DR OMA; CCKQSPI; -.
DR OrthoDB; 1296355at2759; -.
DR PhylomeDB; Q8R1R4; -.
DR TreeFam; TF337386; -.
DR Reactome; R-MMU-449836; Other interleukin signaling.
DR BioGRID-ORCS; 76527; 2 hits in 71 CRISPR screens.
DR PRO; PR:Q8R1R4; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8R1R4; protein.
DR Bgee; ENSMUSG00000031750; Expressed in superior frontal gyrus and 82 other tissues.
DR Genevisible; Q8R1R4; MM.
DR GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0005157; F:macrophage colony-stimulating factor receptor binding; ISS:UniProtKB.
DR GO; GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0061514; P:interleukin-34-mediated signaling pathway; ISO:MGI.
DR GO; GO:0061518; P:microglial cell proliferation; IGI:ARUK-UCL.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IDA:ARUK-UCL.
DR GO; GO:0010759; P:positive regulation of macrophage chemotaxis; ISO:MGI.
DR GO; GO:0045651; P:positive regulation of macrophage differentiation; ISO:MGI.
DR GO; GO:0120041; P:positive regulation of macrophage proliferation; ISO:MGI.
DR GO; GO:0043406; P:positive regulation of MAP kinase activity; ISO:MGI.
DR GO; GO:0045657; P:positive regulation of monocyte differentiation; ISO:MGI.
DR GO; GO:0048714; P:positive regulation of oligodendrocyte differentiation; IDA:UniProtKB.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; ISS:UniProtKB.
DR GO; GO:0061098; P:positive regulation of protein tyrosine kinase activity; ISO:MGI.
DR Gene3D; 1.20.1250.80; -; 1.
DR InterPro; IPR020415; IL-34.
DR InterPro; IPR038328; IL-34_sf.
DR PANTHER; PTHR28606; PTHR28606; 1.
DR Pfam; PF15036; IL34; 1.
DR PRINTS; PR01938; INTRLEUKIN34.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cytokine; Glycoprotein; Growth factor;
KW Immunity; Inflammatory response; Innate immunity; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..235
FT /note="Interleukin-34"
FT /id="PRO_0000294349"
FT CARBOHYD 100
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 81
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:18467591"
FT /id="VSP_035080"
FT VAR_SEQ 135..235
FT /note="DVEIGPHVEAVLSLLSTPGLSLKLVRPKALLDNCFRVMELLYCSCCKQSPIL
FT KWQDCELPRLHPHSPGSLMQCTATNVYPLSRQTPTSLPGSPSSSHGSLP -> AVGVHL
FT PGHVLVTLLSQLPGLPSPWARSFDTSWELLMMKGCGDWPSRGSCVISSEYSRPKPEAGA
FT AQSLAGQLLPGHGTAVLFLL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_026632"
FT STRAND 31..33
FT /evidence="ECO:0007829|PDB:4EXN"
FT HELIX 37..44
FT /evidence="ECO:0007829|PDB:4EXN"
FT HELIX 47..54
FT /evidence="ECO:0007829|PDB:4EXN"
FT TURN 55..57
FT /evidence="ECO:0007829|PDB:4EXN"
FT STRAND 64..66
FT /evidence="ECO:0007829|PDB:4EXN"
FT HELIX 68..70
FT /evidence="ECO:0007829|PDB:4EXN"
FT HELIX 74..81
FT /evidence="ECO:0007829|PDB:4EXN"
FT TURN 82..84
FT /evidence="ECO:0007829|PDB:4EXN"
FT HELIX 87..106
FT /evidence="ECO:0007829|PDB:4EXN"
FT HELIX 116..131
FT /evidence="ECO:0007829|PDB:4EXN"
FT TURN 132..135
FT /evidence="ECO:0007829|PDB:4EXN"
FT HELIX 140..150
FT /evidence="ECO:0007829|PDB:4EXN"
FT STRAND 157..159
FT /evidence="ECO:0007829|PDB:4EXN"
FT HELIX 161..180
FT /evidence="ECO:0007829|PDB:4EXN"
FT STRAND 181..184
FT /evidence="ECO:0007829|PDB:4EXN"
FT HELIX 186..188
FT /evidence="ECO:0007829|PDB:4EXN"
FT STRAND 189..191
FT /evidence="ECO:0007829|PDB:4EXP"
SQ SEQUENCE 235 AA; 26764 MW; 4D30E33C977FD54D CRC64;
MPWGLAWLYC LGILLDVALG NENLEIWTLT QDKECDLTGY LRGKLQYKNR LQYMKHYFPI
NYRIAVPYEG VLRVANITRL QKAHVSEREL RYLWVLVSLN ATESVMDVLL EGHPSWKYLQ
EVQTLLENVQ RSLMDVEIGP HVEAVLSLLS TPGLSLKLVR PKALLDNCFR VMELLYCSCC
KQSPILKWQD CELPRLHPHS PGSLMQCTAT NVYPLSRQTP TSLPGSPSSS HGSLP
