IL3_RAT
ID IL3_RAT Reviewed; 166 AA.
AC P04823; P70513;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Interleukin-3;
DE Short=IL-3;
DE AltName: Full=Hematopoietic growth factor;
DE AltName: Full=Mast cell growth factor;
DE Short=MCGF;
DE AltName: Full=Multipotential colony-stimulating factor;
DE AltName: Full=P-cell-stimulating factor;
DE Flags: Precursor;
GN Name=Il3; Synonyms=Il-3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3086845; DOI=10.1093/nar/14.9.3641;
RA Cohen D.R., Hapel A.J., Young I.G.;
RT "Cloning and expression of the rat interleukin-3 gene.";
RL Nucleic Acids Res. 14:3641-3658(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9747458; DOI=10.1038/sj.gt.3300662;
RA Esandi M.C., van Someren G.D., Bout A., Mulder A.H., van Bekkum D.W.,
RA Valerio D., Noteboom J.L.;
RT "IL-1/IL-3 gene therapy of non-small cell lung cancer (NSCLC) in rats using
RT 'cracked' adenoproducer cells.";
RL Gene Ther. 5:778-788(1998).
RN [3]
RP FUNCTION.
RX PubMed=34183475;
RA Piprode V., Singh K., Kumar A., Josh S.R., Wani M.R.;
RT "IL-3 inhibits rat osteoclast differentiation induced by TNF-alpha and
RT other pro-osteoclastogenic cytokines.";
RL J. Biosci. 46:0-0(2021).
CC -!- FUNCTION: Cytokine secreted predominantly by activated T-lymphocytes as
CC well as mast cells and osteoblastic cells that controls the production
CC and differentiation of hematopoietic progenitor cells into lineage-
CC restricted cells. Stimulates also mature basophils, eosinophils, and
CC monocytes to become functionally activated. In addition, plays an
CC important role in neural cell proliferation and survival. Participates
CC as well in bone homeostasis and inhibits osteoclast differentiation by
CC preventing NF-kappa-B nuclear translocation and activation
CC (PubMed:34183475). Mechanistically, exerts its biological effects
CC through a receptor composed of IL3RA subunit and a signal transducing
CC subunit IL3RB (By similarity). Receptor stimulation results in the
CC rapid activation of JAK2 kinase activity leading to STAT5-mediated
CC transcriptional program. Alternatively, contributes to cell survival
CC under oxidative stress in non-hematopoietic systems by activating
CC pathways mediated by PI3K/AKT and ERK (By similarity).
CC {ECO:0000250|UniProtKB:P08700}.
CC -!- SUBUNIT: Monomer.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Activated T-cells, mast cells, natural killer
CC cells.
CC -!- SIMILARITY: Belongs to the IL-3 family. {ECO:0000305}.
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DR EMBL; X03846; CAA27473.1; -; Genomic_DNA.
DR EMBL; X03914; CAA27549.1; -; Genomic_DNA.
DR EMBL; U81493; AAC17705.1; -; mRNA.
DR PIR; S07369; S07369.
DR AlphaFoldDB; P04823; -.
DR SMR; P04823; -.
DR STRING; 10116.ENSRNOP00000030704; -.
DR GlyGen; P04823; 2 sites.
DR PRIDE; P04823; -.
DR UCSC; RGD:2897; rat.
DR RGD; 2897; Il3.
DR InParanoid; P04823; -.
DR PhylomeDB; P04823; -.
DR PRO; PR:P04823; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005615; C:extracellular space; IDA:RGD.
DR GO; GO:0005125; F:cytokine activity; IDA:RGD.
DR GO; GO:0008083; F:growth factor activity; ISO:RGD.
DR GO; GO:0005135; F:interleukin-3 receptor binding; IMP:RGD.
DR GO; GO:0042976; P:activation of Janus kinase activity; ISO:RGD.
DR GO; GO:0019221; P:cytokine-mediated signaling pathway; ISO:RGD.
DR GO; GO:0035162; P:embryonic hemopoiesis; ISO:RGD.
DR GO; GO:0097192; P:extrinsic apoptotic signaling pathway in absence of ligand; ISO:RGD.
DR GO; GO:0030097; P:hemopoiesis; IMP:RGD.
DR GO; GO:0006955; P:immune response; IEA:InterPro.
DR GO; GO:0038156; P:interleukin-3-mediated signaling pathway; ISO:RGD.
DR GO; GO:0030224; P:monocyte differentiation; ISO:RGD.
DR GO; GO:0033028; P:myeloid cell apoptotic process; ISO:RGD.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:RGD.
DR GO; GO:0010507; P:negative regulation of autophagy; ISO:RGD.
DR GO; GO:0033026; P:negative regulation of mast cell apoptotic process; ISO:RGD.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:RGD.
DR GO; GO:0046330; P:positive regulation of JNK cascade; ISO:RGD.
DR GO; GO:0070668; P:positive regulation of mast cell proliferation; ISO:RGD.
DR GO; GO:0002763; P:positive regulation of myeloid leukocyte differentiation; ISO:RGD.
DR GO; GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; ISS:UniProtKB.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; ISO:RGD.
DR GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; ISO:RGD.
DR GO; GO:0001558; P:regulation of cell growth; IMP:RGD.
DR GO; GO:0010468; P:regulation of gene expression; ISO:RGD.
DR GO; GO:0006110; P:regulation of glycolytic process; ISO:RGD.
DR GO; GO:0035304; P:regulation of protein dephosphorylation; ISO:RGD.
DR GO; GO:0009725; P:response to hormone; IMP:RGD.
DR GO; GO:0001666; P:response to hypoxia; ISO:RGD.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR002183; IL-3.
DR Pfam; PF02059; IL3; 1.
DR PIRSF; PIRSF001939; IL-3; 1.
DR PRINTS; PR00430; INTERLEUKIN3.
DR SUPFAM; SSF47266; SSF47266; 1.
PE 2: Evidence at transcript level;
KW Cytokine; Disulfide bond; Glycoprotein; Growth factor; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..166
FT /note="Interleukin-3"
FT /id="PRO_0000015521"
FT REGION 145..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 146..166
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 60
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 70
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 43..106
FT /evidence="ECO:0000250"
FT DISULFID 105..166
FT /evidence="ECO:0000250"
SQ SEQUENCE 166 AA; 18631 MW; 56A564F3D2CCF757 CRC64;
MVLASSTTSI LCMLLPLLML FHQGLQISDR GSDAHHLLRT LDCRTIALEI LVKLPVSGLN
NSDDKANLRN STLRRVNLDE FLKSQEEFDS QDTTDIKSKL QKLKCCIPAA ASDSVLPGVY
NKDLDDFKKK LRFYVIHLKD LQPVSVSRPP QPTSSSDNFR PMTVEC
