APNL_BRUA4
ID APNL_BRUA4 Reviewed; 609 AA.
AC A6X3G3;
DT 16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=D-apionate lactonase {ECO:0000303|PubMed:29867142};
DE EC=3.1.1.115 {ECO:0000269|PubMed:29867142};
GN Name=apnL {ECO:0000303|PubMed:29867142};
GN OrderedLocusNames=Oant_3059 {ECO:0000312|EMBL:ABS15767.1};
OS Brucella anthropi (strain ATCC 49188 / DSM 6882 / CCUG 24695 / JCM 21032 /
OS LMG 3331 / NBRC 15819 / NCTC 12168 / Alc 37) (Ochrobactrum anthropi).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=439375;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49188 / DSM 6882 / CCUG 24695 / JCM 21032 / LMG 3331 / NBRC
RC 15819 / NCTC 12168 / Alc 37;
RX PubMed=21685287; DOI=10.1128/jb.05335-11;
RA Chain P.S., Lang D.M., Comerci D.J., Malfatti S.A., Vergez L.M., Shin M.,
RA Ugalde R.A., Garcia E., Tolmasky M.E.;
RT "Genome of Ochrobactrum anthropi ATCC 49188 T, a versatile opportunistic
RT pathogen and symbiont of several eukaryotic hosts.";
RL J. Bacteriol. 193:4274-4275(2011).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=29867142; DOI=10.1038/s41589-018-0067-7;
RA Carter M.S., Zhang X., Huang H., Bouvier J.T., Francisco B.S.,
RA Vetting M.W., Al-Obaidi N., Bonanno J.B., Ghosh A., Zallot R.G.,
RA Andersen H.M., Almo S.C., Gerlt J.A.;
RT "Functional assignment of multiple catabolic pathways for D-apiose.";
RL Nat. Chem. Biol. 14:696-705(2018).
CC -!- FUNCTION: Involved in catabolism of D-apiose. Hydrolyzes D-
CC apionolactone to D-apionate. {ECO:0000269|PubMed:29867142}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-apionolactone + H2O = D-apionate + H(+);
CC Xref=Rhea:RHEA:57064, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:141216, ChEBI:CHEBI:141352; EC=3.1.1.115;
CC Evidence={ECO:0000269|PubMed:29867142};
CC -!- PATHWAY: Carbohydrate metabolism. {ECO:0000269|PubMed:29867142}.
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DR EMBL; CP000759; ABS15767.1; -; Genomic_DNA.
DR RefSeq; WP_011982774.1; NC_009668.1.
DR AlphaFoldDB; A6X3G3; -.
DR STRING; 439375.Oant_3059; -.
DR EnsemblBacteria; ABS15767; ABS15767; Oant_3059.
DR KEGG; oan:Oant_3059; -.
DR PATRIC; fig|439375.7.peg.3210; -.
DR eggNOG; ENOG502Z7Y3; Bacteria.
DR HOGENOM; CLU_030797_0_0_5; -.
DR OMA; RIGPSTI; -.
DR OrthoDB; 737103at2; -.
DR BioCyc; MetaCyc:MON-20959; -.
DR BRENDA; 3.1.1.115; 4382.
DR Proteomes; UP000002301; Chromosome 2.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Hydrolase; Reference proteome.
FT CHAIN 1..609
FT /note="D-apionate lactonase"
FT /id="PRO_0000446044"
SQ SEQUENCE 609 AA; 66436 MW; AD6E8E9BE4569BB0 CRC64;
MSNVDPFLLY GTRETEAKPT HLKAGLLSLD LNDGNLRTIT YDGVEVLRAV SYLVRDRDWG
TYNPQIHDLN VEQSDSGFIV TYQARCEGPD ATKLTIDVCI QAKSGGTLTF DAVANTATGF
ETNRCGFCIL HPIVGVAGSP VRVEHVDGTL DRTQLPYLIE PWQPFKDMRA ITHEAMPGVT
AECRMEGDTF EMEDQRNWSD ASYKTYVRPL ALPWPYQIEA SKPQHQRIVL NISDTRKAAL
QAGMNAEPVS ITLGNTSGKL PNIGIIITPE EAKASLAAID LLQEIDPQDL LFQYDPMAGH
NGSAFADFAT LAAKHSARVS LEIALPCEKS LIEETTAIAS DMKAAGFNPD AVIVSPAIDR
QSTPPGSEWP TCPPLEDVYA AARAAFPNAR LGGGMLSYFT ELNRKCVPGE LVDFVTHCTN
PIVHAADDLS VMQTLEALPF ITRSVRAVYG DKPYRIGPST IPMRQNPYGS RTMENPNGKR
IAMANRDPRH NGKFAESFAL AYAISVLNAG LDSLTLSALT GPFGLTAGEG EPTLAGGKRP
LFTTIKTLSG LAGKDWWQLI SSRPDHVLAF ATEHEFWLVN ITSQPQTVSI AQFAKITLDP
YAVQNLKRS
