IL5RA_MOUSE
ID IL5RA_MOUSE Reviewed; 415 AA.
AC P21183;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1991, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Interleukin-5 receptor subunit alpha;
DE Short=IL-5 receptor subunit alpha;
DE Short=IL-5R subunit alpha;
DE Short=IL-5R-alpha;
DE Short=IL-5RA;
DE AltName: CD_antigen=CD125;
DE Flags: Precursor;
GN Name=Il5ra; Synonyms=Il5r;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2265612; DOI=10.1002/j.1460-2075.1990.tb07886.x;
RA Takaki S., Tominaga A., Mita S., Sonoda E., Yamaguchi N., Takatsu K.;
RT "Molecular cloning and expression of the murine interleukin-5 receptor.";
RL EMBO J. 9:4367-4374(1990).
RN [2]
RP FUNCTION, AND INTERACTION WITH IL5.
RX PubMed=1873482; DOI=10.1016/1043-4666(91)90503-6;
RA Barry S.C., McKenzie A.N., Strath M., Sanderson C.J.;
RT "Analysis of interleukin 5 receptors on murine eosinophils: a comparison
RT with receptors on B13 cells.";
RL Cytokine 3:339-344(1991).
CC -!- FUNCTION: Cell surface receptor that plays an important role in the
CC survival, differentiation, and chemotaxis of eosinophils
CC (PubMed:1873482). Acts by forming an heterodimeric receptor with CSF2RB
CC subunit and subsequently binding to interleukin-5. In unstimulated
CC conditions, interacts constitutively with JAK2. Heterodimeric receptor
CC activation leads to JAK2 stimulation and subsequent activation of the
CC JAK-STAT pathway. {ECO:0000250|UniProtKB:Q01344,
CC ECO:0000269|PubMed:1873482}.
CC -!- SUBUNIT: Interacts with IL5 (PubMed:1873482). Interacts with CSF2RB.
CC Interacts with JAK2. Interacts with SDCBP.
CC {ECO:0000250|UniProtKB:Q01344, ECO:0000269|PubMed:1873482}.
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed on eosinophils and basophils. Also on B-
CC cells.
CC -!- DOMAIN: The WSXWS motif appears to be necessary for proper protein
CC folding and thereby efficient intracellular transport and cell-surface
CC receptor binding.
CC -!- DOMAIN: The box 1 motif is required for JAK interaction and/or
CC activation.
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DR EMBL; D90205; BAA14231.1; -; mRNA.
DR CCDS; CCDS20396.1; -.
DR PIR; S12357; S12357.
DR RefSeq; NP_032396.1; NM_008370.2.
DR RefSeq; XP_017176895.1; XM_017321406.1.
DR AlphaFoldDB; P21183; -.
DR SMR; P21183; -.
DR STRING; 10090.ENSMUSP00000129781; -.
DR BindingDB; P21183; -.
DR ChEMBL; CHEMBL4523198; -.
DR GlyGen; P21183; 4 sites.
DR PhosphoSitePlus; P21183; -.
DR PaxDb; P21183; -.
DR PRIDE; P21183; -.
DR ProteomicsDB; 267048; -.
DR Antibodypedia; 2274; 413 antibodies from 34 providers.
DR DNASU; 16192; -.
DR Ensembl; ENSMUST00000167925; ENSMUSP00000129781; ENSMUSG00000005364.
DR Ensembl; ENSMUST00000204659; ENSMUSP00000144718; ENSMUSG00000005364.
DR GeneID; 16192; -.
DR KEGG; mmu:16192; -.
DR UCSC; uc009dcx.1; mouse.
DR CTD; 3568; -.
DR MGI; MGI:96558; Il5ra.
DR VEuPathDB; HostDB:ENSMUSG00000005364; -.
DR eggNOG; ENOG502QZNV; Eukaryota.
DR GeneTree; ENSGT00940000160890; -.
DR HOGENOM; CLU_039945_0_0_1; -.
DR InParanoid; P21183; -.
DR OMA; HAIDQVN; -.
DR OrthoDB; 1151666at2759; -.
DR PhylomeDB; P21183; -.
DR TreeFam; TF331549; -.
DR Reactome; R-MMU-512988; Interleukin-3, Interleukin-5 and GM-CSF signaling.
DR Reactome; R-MMU-5673001; RAF/MAP kinase cascade.
DR Reactome; R-MMU-912526; Interleukin receptor SHC signaling.
DR BioGRID-ORCS; 16192; 3 hits in 72 CRISPR screens.
DR PRO; PR:P21183; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; P21183; protein.
DR Bgee; ENSMUSG00000005364; Expressed in right kidney and 12 other tissues.
DR ExpressionAtlas; P21183; baseline and differential.
DR GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043235; C:receptor complex; IBA:GO_Central.
DR GO; GO:0019955; F:cytokine binding; IBA:GO_Central.
DR GO; GO:0004896; F:cytokine receptor activity; IBA:GO_Central.
DR GO; GO:0071310; P:cellular response to organic substance; IMP:MGI.
DR GO; GO:0019221; P:cytokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0002437; P:inflammatory response to antigenic stimulus; IMP:MGI.
DR GO; GO:0032674; P:regulation of interleukin-5 production; IMP:MGI.
DR CDD; cd00063; FN3; 1.
DR Gene3D; 2.60.40.10; -; 3.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003532; Short_hematopoietin_rcpt_2_CS.
DR InterPro; IPR015321; TypeI_recpt_CBD.
DR Pfam; PF09240; IL6Ra-bind; 1.
DR SUPFAM; SSF49265; SSF49265; 2.
DR PROSITE; PS50853; FN3; 2.
DR PROSITE; PS01356; HEMATOPO_REC_S_F2; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Glycoprotein; Membrane; Receptor; Reference proteome;
KW Repeat; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..17
FT CHAIN 18..415
FT /note="Interleukin-5 receptor subunit alpha"
FT /id="PRO_0000010894"
FT TOPO_DOM 18..339
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 362..415
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 29..120
FT /note="Fibronectin type-III 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 238..331
FT /note="Fibronectin type-III 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT MOTIF 319..323
FT /note="WSXWS motif"
FT MOTIF 367..375
FT /note="Box 1 motif"
FT CARBOHYD 32
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 128
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 213
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 241
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 131..152
FT /evidence="ECO:0000250"
FT DISULFID 179..193
FT /evidence="ECO:0000250"
FT DISULFID 266..313
FT /evidence="ECO:0000250"
SQ SEQUENCE 415 AA; 46989 MW; A4326D2922571C08 CRC64;
MVPVLLILVG ALATLQADLL NHKKFLLLPP VNFTIKATGL AQVLLHWDPN PDQEQRHVDL
EYHVKINAPQ EDEYDTRKTE SKCVTPLHEG FAASVRTILK SSHTTLASSW VSAELKAPPG
SPGTSVTNLT CTTHTVVSSH THLRPYQVSL RCTWLVGKDA PEDTQYFLYY RFGVLTEKCQ
EYSRDALNRN TACWFPRTFI NSKGFEQLAV HINGSSKRAA IKPFDQLFSP LAIDQVNPPR
NVTVEIESNS LYIQWEKPLS AFPDHCFNYE LKIYNTKNGH IQKEKLIANK FISKIDDVST
YSIQVRAAVS SPCRMPGRWG EWSQPIYVGK ERKSLVEWHL IVLPTAACFV LLIFSLICRV
CHLWTRLFPP VPAPKSNIKD LPVVTEYEKP SNETKIEVVH CVEEVGFEVM GNSTF
