APN_DROME
ID APN_DROME Reviewed; 137 AA.
AC Q9VFX3;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Protein apnoia {ECO:0000303|PubMed:30645584};
GN Name=apn {ECO:0000303|PubMed:30645584, ECO:0000312|FlyBase:FBgn0038132};
GN ORFNames=CG15887 {ECO:0000312|FlyBase:FBgn0038132};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL68313.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:ANY27178.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:ANY27178.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000312|EMBL:ANY27178.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:ANY27178.1};
RA Florea S., Webb J.S., Jaromczyk J., Schardl C.L.;
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, INTERACTION WITH CRB, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE,
RP AND SUBCELLULAR LOCATION.
RX PubMed=30645584; DOI=10.1371/journal.pgen.1007852;
RA Skouloudaki K., Papadopoulos D.K., Tomancak P., Knust E.;
RT "The apical protein Apnoia interacts with Crumbs to regulate tracheal
RT growth and inflation.";
RL PLoS Genet. 15:E1007852-E1007852(2019).
CC -!- FUNCTION: Transmembrane protein that plays a key role in trachea
CC development by regulating crb localization and maintenance at the
CC apical cell membrane (PubMed:30645584). Required for anisotropic apical
CC surface expansion important for tracheal tube elongation and lumen
CC stability at larval stages (PubMed:30645584).
CC {ECO:0000269|PubMed:30645584}.
CC -!- SUBUNIT: Interacts with crb. {ECO:0000269|PubMed:30645584}.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane
CC {ECO:0000269|PubMed:30645584}; Multi-pass membrane protein
CC {ECO:0000255}. Note=In tracheal cells, colocalizes with crb in the
CC subapical region, a small region of the apical membrane apical to the
CC adherens junctions. {ECO:0000269|PubMed:30645584}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryos at stage 13 in tracheal
CC fusion cells (at protein level). During embryonic stages 15 and 16,
CC detected in the dorsal and lateral trunks, in the visceral and dorsal
CC branches and in the transverse connective branches (at protein level).
CC In the larvae, continuously expressed in the entire tracheal system (at
CC protein level). {ECO:0000269|PubMed:30645584}.
CC -!- DISRUPTION PHENOTYPE: Dies at second instar larval stage
CC (PubMed:30645584). Shows reduced larval body size and shortened dorsal
CC trunk probably due to defective membrane growth in larval tracheae
CC (PubMed:30645584). Results in defective tracheal tube maturation
CC including liquid clearance and gas filling resulting in twisted and
CC uninflated tracheal tubes (PubMed:30645584). Results in mislocalization
CC of crb from the subapical region to Vsp35/retromer-positive vesicles in
CC the cytoplasm (PubMed:30645584). RNAi-mediated knockdown in the trachea
CC results in a similar phenotype (PubMed:30645584).
CC {ECO:0000269|PubMed:30645584}.
CC -!- MISCELLANEOUS: Apnoia means lack of air in Greek.
CC {ECO:0000303|PubMed:30645584}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014297; AAF54922.1; -; Genomic_DNA.
DR EMBL; AY075504; AAL68313.1; -; mRNA.
DR EMBL; KX531368; ANY27178.1; -; mRNA.
DR RefSeq; NP_650270.1; NM_142013.3.
DR AlphaFoldDB; Q9VFX3; -.
DR IntAct; Q9VFX3; 1.
DR STRING; 7227.FBpp0082244; -.
DR TCDB; 9.B.326.1.1; the transmembrane apical trachael tube apnoia (apn) family.
DR PaxDb; Q9VFX3; -.
DR PRIDE; Q9VFX3; -.
DR DNASU; 41631; -.
DR EnsemblMetazoa; FBtr0082776; FBpp0082244; FBgn0038132.
DR GeneID; 41631; -.
DR KEGG; dme:Dmel_CG15887; -.
DR UCSC; CG15887-RA; d. melanogaster.
DR CTD; 41631; -.
DR FlyBase; FBgn0038132; apn.
DR VEuPathDB; VectorBase:FBgn0038132; -.
DR eggNOG; ENOG502SXCA; Eukaryota.
DR HOGENOM; CLU_1961417_0_0_1; -.
DR InParanoid; Q9VFX3; -.
DR OMA; CSVNRIT; -.
DR OrthoDB; 1553400at2759; -.
DR PhylomeDB; Q9VFX3; -.
DR BioGRID-ORCS; 41631; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41631; -.
DR PRO; PR:Q9VFX3; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0038132; Expressed in embryonic/larval trachea (Drosophila) and 11 other tissues.
DR GO; GO:0016324; C:apical plasma membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0035001; P:dorsal trunk growth, open tracheal system; IMP:UniProtKB.
DR GO; GO:0035002; P:liquid clearance, open tracheal system; IMP:UniProtKB.
DR GO; GO:0035149; P:lumen formation, open tracheal system; IMP:UniProtKB.
DR GO; GO:0007424; P:open tracheal system development; IMP:UniProtKB.
DR GO; GO:1905477; P:positive regulation of protein localization to membrane; IMP:UniProtKB.
DR GO; GO:0060438; P:trachea development; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..137
FT /note="Protein apnoia"
FT /evidence="ECO:0000255"
FT /id="PRO_5015100241"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 137 AA; 14446 MW; C1B50B8C6886E648 CRC64;
MAANQKIVFA LVCLFLACDL VLGQQQAANS SDSDSDVAES RTFGHHFLRR ISFALVPGAF
VVGVITTLLA ALTVVSIKGL GVGVILLVLA IGQMLSRALP VQAAAAYAAA PVPVQAPVPV
VYSHSHTQQP VWLEKEW
