ID   IL8_DASNO               Reviewed;          96 AA.
AC   Q102R3;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Interleukin-8;
DE            Short=IL-8;
DE   AltName: Full=C-X-C motif chemokine 8;
DE   AltName: Full=Chemokine (C-X-C motif) ligand 8;
DE   Flags: Precursor;
GN   Name=CXCL8; Synonyms=IL8;
OS   Dasypus novemcinctus (Nine-banded armadillo).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Xenarthra; Cingulata; Dasypodidae; Dasypus.
OX   NCBI_TaxID=9361;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Adams J.E.;
RT   "Overexpression of Dasypus novemcinctus IL-8 in E. coli.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Chemotactic factor that mediates inflammatory response by
CC       attracting neutrophils, basophils, and T-cells to clear pathogens and
CC       protect the host from infection. Also plays an important role in
CC       neutrophil activation. Released in response to an inflammatory
CC       stimulus, exerts its effect by binding to the G-protein-coupled
CC       receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes
CC       and endothelial cells. G-protein heterotrimer (alpha, beta, gamma
CC       subunits) constitutively binds to CXCR1/CXCR2 receptor and activation
CC       by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in
CC       neutrophils) and activation of several downstream signaling pathways
CC       including PI3K and MAPK pathways. {ECO:0000250|UniProtKB:P10145}.
CC   -!- SUBUNIT: Homodimer. Interacts with TNFAIP6 (via Link domain); this
CC       interaction interferes with chemokine binding to glycosaminoglycans.
CC       {ECO:0000250|UniProtKB:P10145}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: Citrullination at Arg-27 prevents proteolysis, and dampens tissue
CC       inflammation, it also enhances leukocytosis, possibly through impaired
CC       chemokine clearance from the blood circulation. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family.
CC       {ECO:0000305}.
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DR   EMBL; DQ099902; AAZ76732.1; -; mRNA.
DR   RefSeq; NP_001268240.1; NM_001281311.1.
DR   AlphaFoldDB; Q102R3; -.
DR   SMR; Q102R3; -.
DR   GeneID; 101441641; -.
DR   KEGG; dnm:101441641; -.
DR   CTD; 3576; -.
DR   OrthoDB; 1618797at2759; -.
DR   TreeFam; TF333433; -.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR   GO; GO:0008009; F:chemokine activity; IEA:InterPro.
DR   GO; GO:0008201; F:heparin binding; ISS:UniProtKB.
DR   GO; GO:0005153; F:interleukin-8 receptor binding; IEA:InterPro.
DR   GO; GO:0006955; P:immune response; IEA:InterPro.
DR   GO; GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
DR   GO; GO:0042119; P:neutrophil activation; IEA:InterPro.
DR   GO; GO:0030593; P:neutrophil chemotaxis; ISS:UniProtKB.
DR   CDD; cd00273; Chemokine_CXC; 1.
DR   InterPro; IPR001089; Chemokine_CXC.
DR   InterPro; IPR018048; Chemokine_CXC_CS.
DR   InterPro; IPR001811; Chemokine_IL8-like_dom.
DR   InterPro; IPR033899; CXC_Chemokine_domain.
DR   InterPro; IPR028469; Interleukin-8.
DR   InterPro; IPR036048; Interleukin_8-like_sf.
DR   PANTHER; PTHR10179; PTHR10179; 1.
DR   PANTHER; PTHR10179:SF42; PTHR10179:SF42; 1.
DR   Pfam; PF00048; IL8; 1.
DR   PRINTS; PR00437; SMALLCYTKCXC.
DR   SMART; SM00199; SCY; 1.
DR   SUPFAM; SSF54117; SSF54117; 1.
DR   PROSITE; PS00471; SMALL_CYTOKINES_CXC; 1.
PE   3: Inferred from homology;
KW   Chemotaxis; Citrullination; Cytokine; Disulfide bond;
KW   Inflammatory response; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..96
FT                   /note="Interleukin-8"
FT                   /id="PRO_0000253755"
FT   MOD_RES         27
FT                   /note="Citrulline"
FT                   /evidence="ECO:0000250"
FT   DISULFID        34..61
FT                   /evidence="ECO:0000250"
FT   DISULFID        36..78
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   96 AA;  10699 MW;  5776B85A662DE696 CRC64;
     MTSKLAIALL ATFMLSAALC EAAVLSRSGT ELRCLCINTH STYFHPRVIK ELRVIESGPH
     CPNTEIMSVK LVNGAEVCLD PKQKWVQKVV ELFLKR