ID   IL8_HORSE               Reviewed;         101 AA.
AC   O62812; Q866R3;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 2.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Interleukin-8;
DE            Short=IL-8;
DE   AltName: Full=C-X-C motif chemokine 8;
DE   AltName: Full=Chemokine (C-X-C motif) ligand 8;
DE   Flags: Precursor;
GN   Name=CXCL8; Synonyms=IL8;
OS   Equus caballus (Horse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Cappelli K., Verini-Supplizi A., Silvestrelli M.;
RT   "Effects of exercise on transcriptional profiles in endurance horses.";
RL   Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-97.
RA   Franchini M.;
RL   Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Chemotactic factor that mediates inflammatory response by
CC       attracting neutrophils, basophils, and T-cells to clear pathogens and
CC       protect the host from infection. Also plays an important role in
CC       neutrophil activation. Released in response to an inflammatory
CC       stimulus, exerts its effect by binding to the G-protein-coupled
CC       receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes
CC       and endothelial cells. G-protein heterotrimer (alpha, beta, gamma
CC       subunits) constitutively binds to CXCR1/CXCR2 receptor and activation
CC       by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in
CC       neutrophils) and activation of several downstream signaling pathways
CC       including PI3K and MAPK pathways. {ECO:0000250|UniProtKB:P10145}.
CC   -!- SUBUNIT: Homodimer. Interacts with TNFAIP6 (via Link domain); this
CC       interaction interferes with chemokine binding to glycosaminoglycans.
CC       {ECO:0000250|UniProtKB:P10145}.
CC   -!- INTERACTION:
CC       O62812; P28967: gG; Xeno; NbExp=3; IntAct=EBI-11701719, EBI-11701747;
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: Citrullination at Arg-27 prevents proteolysis, and dampens tissue
CC       inflammation, it also enhances leukocytosis, possibly through impaired
CC       chemokine clearance from the blood circulation. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family.
CC       {ECO:0000305}.
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DR   EMBL; AY184956; AAO37764.1; -; mRNA.
DR   EMBL; AF062377; AAC16015.1; -; mRNA.
DR   RefSeq; NP_001077420.2; NM_001083951.2.
DR   AlphaFoldDB; O62812; -.
DR   SMR; O62812; -.
DR   IntAct; O62812; 1.
DR   STRING; 9796.ENSECAP00000013083; -.
DR   PaxDb; O62812; -.
DR   GeneID; 100037400; -.
DR   KEGG; ecb:100037400; -.
DR   CTD; 3576; -.
DR   InParanoid; O62812; -.
DR   OrthoDB; 1618797at2759; -.
DR   Proteomes; UP000002281; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0008009; F:chemokine activity; IBA:GO_Central.
DR   GO; GO:0045236; F:CXCR chemokine receptor binding; IBA:GO_Central.
DR   GO; GO:0008201; F:heparin binding; ISS:UniProtKB.
DR   GO; GO:0005153; F:interleukin-8 receptor binding; IEA:InterPro.
DR   GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR   GO; GO:0071222; P:cellular response to lipopolysaccharide; IBA:GO_Central.
DR   GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR   GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR   GO; GO:0042119; P:neutrophil activation; IEA:InterPro.
DR   GO; GO:0030593; P:neutrophil chemotaxis; ISS:UniProtKB.
DR   CDD; cd00273; Chemokine_CXC; 1.
DR   InterPro; IPR001089; Chemokine_CXC.
DR   InterPro; IPR018048; Chemokine_CXC_CS.
DR   InterPro; IPR001811; Chemokine_IL8-like_dom.
DR   InterPro; IPR033899; CXC_Chemokine_domain.
DR   InterPro; IPR028469; Interleukin-8.
DR   InterPro; IPR036048; Interleukin_8-like_sf.
DR   PANTHER; PTHR10179; PTHR10179; 1.
DR   PANTHER; PTHR10179:SF42; PTHR10179:SF42; 1.
DR   Pfam; PF00048; IL8; 1.
DR   PRINTS; PR00437; SMALLCYTKCXC.
DR   SMART; SM00199; SCY; 1.
DR   SUPFAM; SSF54117; SSF54117; 1.
DR   PROSITE; PS00471; SMALL_CYTOKINES_CXC; 1.
PE   1: Evidence at protein level;
KW   Chemotaxis; Citrullination; Cytokine; Disulfide bond;
KW   Inflammatory response; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000250"
FT   CHAIN           23..101
FT                   /note="Interleukin-8"
FT                   /id="PRO_0000005125"
FT   MOD_RES         27
FT                   /note="Citrulline"
FT                   /evidence="ECO:0000250"
FT   DISULFID        34..61
FT                   /evidence="ECO:0000250"
FT   DISULFID        36..77
FT                   /evidence="ECO:0000250"
FT   CONFLICT        54
FT                   /note="A -> V (in Ref. 1; AAC16015)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        96
FT                   /note="A -> T (in Ref. 1; AAC16015)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   101 AA;  11080 MW;  E34878111854009A CRC64;
     MTSKLAVALL AVFLLSAALC EAAVVSRITA ELRCQCIKTH SKPFNPKLIK EMRAIESGPH
     CENSEIIVKL VNGAEVCLNP HTKWVQIIVQ AFLKRAEGQN P