IL8_SHEEP
ID IL8_SHEEP Reviewed; 101 AA.
AC P36925;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Interleukin-8;
DE Short=IL-8;
DE AltName: Full=C-X-C motif chemokine 8;
DE AltName: Full=Chemokine (C-X-C motif) ligand 8;
DE Flags: Precursor;
GN Name=CXCL8; Synonyms=IL8;
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7821808; DOI=10.1016/0378-1119(94)90454-5;
RA Legastelois I., Greenland T., Arnaud P., Mornex J.F., Cordier G.;
RT "Sequencing of the ovine interleukin-8-encoding cDNA using the polymerase
RT chain reaction.";
RL Gene 150:367-369(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7835984; DOI=10.1038/icb.1994.59;
RA Seow H.F., Yoshimura T., Wood P.R., Colditz I.G.;
RT "Cloning, sequencing, expression and inflammatory activity in skin of ovine
RT interleukin-8.";
RL Immunol. Cell Biol. 72:398-405(1994).
CC -!- FUNCTION: Chemotactic factor that mediates inflammatory response by
CC attracting neutrophils, basophils, and T-cells to clear pathogens and
CC protect the host from infection. Also plays an important role in
CC neutrophil activation. Released in response to an inflammatory
CC stimulus, exerts its effect by binding to the G-protein-coupled
CC receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes
CC and endothelial cells. G-protein heterotrimer (alpha, beta, gamma
CC subunits) constitutively binds to CXCR1/CXCR2 receptor and activation
CC by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in
CC neutrophils) and activation of several downstream signaling pathways
CC including PI3K and MAPK pathways. {ECO:0000250|UniProtKB:P10145}.
CC -!- SUBUNIT: Homodimer. Interacts with TNFAIP6 (via Link domain); this
CC interaction interferes with chemokine binding to glycosaminoglycans.
CC {ECO:0000250|UniProtKB:P10145}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- PTM: Citrullination at Arg-27 prevents proteolysis, and dampens tissue
CC inflammation, it also enhances leukocytosis, possibly through impaired
CC chemokine clearance from the blood circulation. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family.
CC {ECO:0000305}.
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DR EMBL; X78306; CAA55115.1; -; mRNA.
DR EMBL; S74436; AAB33241.1; -; mRNA.
DR PIR; S42496; S42496.
DR RefSeq; NP_001009401.1; NM_001009401.2.
DR AlphaFoldDB; P36925; -.
DR SMR; P36925; -.
DR STRING; 9940.ENSOARP00000015557; -.
DR Ensembl; ENSOART00000015785; ENSOARP00000015557; ENSOARG00000014496.
DR Ensembl; ENSOART00020004439; ENSOARP00020003646; ENSOARG00020002886.
DR GeneID; 443418; -.
DR KEGG; oas:443418; -.
DR CTD; 3576; -.
DR eggNOG; ENOG502S7MM; Eukaryota.
DR HOGENOM; CLU_143902_3_0_1; -.
DR OMA; IGTELRC; -.
DR OrthoDB; 1618797at2759; -.
DR Proteomes; UP000002356; Chromosome 6.
DR Bgee; ENSOARG00000014496; Expressed in caecum and 49 other tissues.
DR ExpressionAtlas; P36925; baseline.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR GO; GO:0008009; F:chemokine activity; IEA:Ensembl.
DR GO; GO:0008201; F:heparin binding; ISS:UniProtKB.
DR GO; GO:0005153; F:interleukin-8 receptor binding; ISS:UniProtKB.
DR GO; GO:0044344; P:cellular response to fibroblast growth factor stimulus; IEA:Ensembl.
DR GO; GO:0071347; P:cellular response to interleukin-1; IEA:Ensembl.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IEA:Ensembl.
DR GO; GO:0071356; P:cellular response to tumor necrosis factor; IEA:Ensembl.
DR GO; GO:0048566; P:embryonic digestive tract development; IEA:Ensembl.
DR GO; GO:0006955; P:immune response; IEA:InterPro.
DR GO; GO:0050930; P:induction of positive chemotaxis; ISS:UniProtKB.
DR GO; GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:Ensembl.
DR GO; GO:0060354; P:negative regulation of cell adhesion molecule production; IEA:Ensembl.
DR GO; GO:0045744; P:negative regulation of G protein-coupled receptor signaling pathway; IEA:Ensembl.
DR GO; GO:0010629; P:negative regulation of gene expression; IEA:Ensembl.
DR GO; GO:0042119; P:neutrophil activation; IEA:Ensembl.
DR GO; GO:0030593; P:neutrophil chemotaxis; ISS:UniProtKB.
DR GO; GO:0045766; P:positive regulation of angiogenesis; IEA:Ensembl.
DR GO; GO:0031328; P:positive regulation of cellular biosynthetic process; IEA:Ensembl.
DR GO; GO:0010628; P:positive regulation of gene expression; IEA:Ensembl.
DR GO; GO:0031623; P:receptor internalization; IEA:Ensembl.
DR GO; GO:0030155; P:regulation of cell adhesion; ISS:UniProtKB.
DR GO; GO:2000535; P:regulation of entry of bacterium into host cell; IEA:Ensembl.
DR GO; GO:0045091; P:regulation of single stranded viral RNA replication via double stranded DNA intermediate; ISS:UniProtKB.
DR GO; GO:0034976; P:response to endoplasmic reticulum stress; IEA:Ensembl.
DR CDD; cd00273; Chemokine_CXC; 1.
DR InterPro; IPR001089; Chemokine_CXC.
DR InterPro; IPR018048; Chemokine_CXC_CS.
DR InterPro; IPR001811; Chemokine_IL8-like_dom.
DR InterPro; IPR033899; CXC_Chemokine_domain.
DR InterPro; IPR028469; Interleukin-8.
DR InterPro; IPR036048; Interleukin_8-like_sf.
DR PANTHER; PTHR10179; PTHR10179; 1.
DR PANTHER; PTHR10179:SF42; PTHR10179:SF42; 1.
DR Pfam; PF00048; IL8; 1.
DR PRINTS; PR00437; SMALLCYTKCXC.
DR SMART; SM00199; SCY; 1.
DR SUPFAM; SSF54117; SSF54117; 1.
DR PROSITE; PS00471; SMALL_CYTOKINES_CXC; 1.
PE 3: Inferred from homology;
KW Chemotaxis; Citrullination; Cytokine; Disulfide bond;
KW Inflammatory response; Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000250"
FT CHAIN 23..101
FT /note="Interleukin-8"
FT /id="PRO_0000005136"
FT MOD_RES 27
FT /note="Citrulline"
FT /evidence="ECO:0000250"
FT DISULFID 34..61
FT /evidence="ECO:0000250"
FT DISULFID 36..77
FT /evidence="ECO:0000250"
SQ SEQUENCE 101 AA; 11292 MW; 40E8418B57C56A5B CRC64;
MTSKLAVALL AAFLLSAALC EAAVLSRMST ELRCQCIKTH STPFHPKFIK ELRVIESGPH
CENSEIIVKL TNGKEVCLDP KEKWVQKVVQ AFLKRAEKQD P
