IL9_MOUSE
ID IL9_MOUSE Reviewed; 144 AA.
AC P15247;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Interleukin-9;
DE Short=IL-9;
DE AltName: Full=Cytokine P40;
DE AltName: Full=T-cell growth factor P40;
DE Flags: Precursor;
GN Name=Il9;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=2521242; DOI=10.1084/jem.169.1.363;
RA van Snick J., Goethals A., Renauld J.-C., van Roost E., Uyttenhove C.,
RA Rubira M.R., Moritz R.L., Simpson R.J.;
RT "Cloning and characterization of a cDNA for a new mouse T cell growth
RT factor (P40).";
RL J. Exp. Med. 169:363-368(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1971295;
RA Renauld J.-C., Goethals A., Houssiau F., Merz H., van Roost E.,
RA van Snick J.;
RT "Human P40/IL-9. Expression in activated CD4+ T cells, genomic
RT organization, and comparison with the mouse gene.";
RL J. Immunol. 144:4235-4241(1990).
RN [3]
RP PROTEIN SEQUENCE OF 19-144, PYROGLUTAMATE FORMATION AT GLN-19, AND
RP GLYCOSYLATION AT ASN-50; ASN-78; ASN-101 AND ASN-114.
RX PubMed=2528451; DOI=10.1111/j.1432-1033.1989.tb21103.x;
RA Simpson R.J., Moritz R.L., Rubira M.R., Gorman J.J., van Snick J.;
RT "Complete amino acid sequence of a new murine T-cell growth factor P40.";
RL Eur. J. Biochem. 183:715-722(1989).
RN [4]
RP PROTEIN SEQUENCE OF 118-136.
RX PubMed=3137580; DOI=10.1073/pnas.85.18.6934;
RA Uyttenhove C., Simpson R.J., van Snick J.;
RT "Functional and structural characterization of P40, a mouse glycoprotein
RT with T-cell growth factor activity.";
RL Proc. Natl. Acad. Sci. U.S.A. 85:6934-6938(1988).
RN [5]
RP FUNCTION, AND INTERACTION WITH IL9R.
RX PubMed=2145361;
RA Druez C., Coulie P., Uyttenhove C., Van Snick J.;
RT "Functional and biochemical characterization of mouse P40/IL-9 receptors.";
RL J. Immunol. 145:2494-2499(1990).
RN [6]
RP FUNCTION, AND INTERACTION WITH IL2RG.
RX PubMed=7718508; DOI=10.1093/intimm/7.1.115;
RA Kimura Y., Takeshita T., Kondo M., Ishii N., Nakamura M., Van Snick J.,
RA Sugamura K.;
RT "Sharing of the IL-2 receptor gamma chain with the functional IL-9 receptor
RT complex.";
RL Int. Immunol. 7:115-120(1995).
RN [7]
RP FUNCTION.
RX PubMed=10464327; DOI=10.1074/jbc.274.36.25855;
RA Demoulin J.B., Van Roost E., Stevens M., Groner B., Renauld J.C.;
RT "Distinct roles for STAT1, STAT3, and STAT5 in differentiation gene
RT induction and apoptosis inhibition by interleukin-9.";
RL J. Biol. Chem. 274:25855-25861(1999).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11070175; DOI=10.1016/s1074-7613(00)00056-x;
RA Townsend J.M., Fallon G.P., Matthews J.D., Smith P., Jolin E.H.,
RA McKenzie N.A.;
RT "IL-9-deficient mice establish fundamental roles for IL-9 in pulmonary
RT mastocytosis and goblet cell hyperplasia but not T cell development.";
RL Immunity 13:573-583(2000).
RN [9]
RP FUNCTION.
RX PubMed=12704113; DOI=10.1128/iai.71.5.2430-2438.2003;
RA Khan W.I., Richard M., Akiho H., Blennerhasset P.A., Humphreys N.E.,
RA Grencis R.K., Van Snick J., Collins S.M.;
RT "Modulation of intestinal muscle contraction by interleukin-9 (IL-9) or IL-
RT 9 neutralization: correlation with worm expulsion in murine nematode
RT infections.";
RL Infect. Immun. 71:2430-2438(2003).
RN [10]
RP FUNCTION.
RX PubMed=19433802; DOI=10.1073/pnas.0812530106;
RA Elyaman W., Bradshaw E.M., Uyttenhove C., Dardalhon V., Awasthi A.,
RA Imitola J., Bettelli E., Oukka M., van Snick J., Renauld J.C.,
RA Kuchroo V.K., Khoury S.J.;
RT "IL-9 induces differentiation of TH17 cells and enhances function of FoxP3+
RT natural regulatory T cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:12885-12890(2009).
CC -!- FUNCTION: Multifunctional cytokine secreted mainly by T-helper 2
CC lymphocytes and also mast cells or NKT cells that plays important roles
CC in the immune response against parasites (PubMed:11070175,
CC PubMed:19433802). Affects intestinal epithelial permeability and
CC adaptive immunity (PubMed:12704113). In addition, induces the
CC differentiation of specific T-cell subsets such as IL-17 producing
CC helper T-cells (TH17) and also proliferation and differentiation of
CC mast cells (PubMed:19433802, PubMed:11070175). Mechanistically, exerts
CC its biological effects through a receptor composed of IL9R subunit and
CC a signal transducing subunit IL2RG (PubMed:2145361, PubMed:7718508).
CC Receptor stimulation results in the rapid activation of JAK1 and JAK3
CC kinase activities leading to STAT1, STAT3 and STAT5-mediated
CC transcriptional programs (PubMed:10464327). Induction of
CC differentiation genes seems to be mediated by STAT1 alone, while
CC protection of cells from apoptosis depends on STAT3 and STAT5
CC (PubMed:10464327). {ECO:0000269|PubMed:10464327,
CC ECO:0000269|PubMed:11070175, ECO:0000269|PubMed:12704113,
CC ECO:0000269|PubMed:19433802, ECO:0000269|PubMed:2145361,
CC ECO:0000269|PubMed:7718508}.
CC -!- SUBUNIT: Interacts with IL9R (PubMed:2145361). Interacts with IL2RG
CC (PubMed:7718508). {ECO:0000269|PubMed:2145361,
CC ECO:0000269|PubMed:7718508}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- DISRUPTION PHENOTYPE: Deltion mice do not show defects in T-cell
CC development or differentiation, the generation of naive or antigen-
CC driven antibody responses, or the expulsion of the intestinal parasitic
CC nematode Nippostrongylus brasiliensis. However, mastocytosis is
CC severely impaired in these animals. {ECO:0000269|PubMed:19433802}.
CC -!- SIMILARITY: Belongs to the IL-7/IL-9 family. {ECO:0000305}.
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DR EMBL; X14045; CAA32203.1; -; mRNA.
DR EMBL; M30136; AAA39874.1; -; Genomic_DNA.
DR CCDS; CCDS26562.1; -.
DR PIR; JL0089; JL0089.
DR RefSeq; NP_032399.1; NM_008373.1.
DR AlphaFoldDB; P15247; -.
DR STRING; 10090.ENSMUSP00000022019; -.
DR GlyGen; P15247; 4 sites.
DR iPTMnet; P15247; -.
DR PhosphoSitePlus; P15247; -.
DR PaxDb; P15247; -.
DR PRIDE; P15247; -.
DR Antibodypedia; 14762; 627 antibodies from 39 providers.
DR DNASU; 16198; -.
DR Ensembl; ENSMUST00000022019; ENSMUSP00000022019; ENSMUSG00000021538.
DR GeneID; 16198; -.
DR KEGG; mmu:16198; -.
DR UCSC; uc007qso.1; mouse.
DR CTD; 3578; -.
DR MGI; MGI:96563; Il9.
DR VEuPathDB; HostDB:ENSMUSG00000021538; -.
DR eggNOG; ENOG502TDE1; Eukaryota.
DR GeneTree; ENSGT00390000015384; -.
DR HOGENOM; CLU_150120_0_0_1; -.
DR InParanoid; P15247; -.
DR OMA; CPFFSCE; -.
DR OrthoDB; 1444257at2759; -.
DR PhylomeDB; P15247; -.
DR TreeFam; TF336367; -.
DR Reactome; R-MMU-8985947; Interleukin-9 signaling.
DR BioGRID-ORCS; 16198; 2 hits in 71 CRISPR screens.
DR PRO; PR:P15247; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; P15247; protein.
DR Bgee; ENSMUSG00000021538; Expressed in entorhinal cortex and 10 other tissues.
DR Genevisible; P15247; MM.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0005125; F:cytokine activity; IGI:MGI.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0005140; F:interleukin-9 receptor binding; ISO:MGI.
DR GO; GO:0030183; P:B cell differentiation; IGI:MGI.
DR GO; GO:0042100; P:B cell proliferation; IGI:MGI.
DR GO; GO:0016064; P:immunoglobulin mediated immune response; IGI:MGI.
DR GO; GO:2001268; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway; IDA:MGI.
DR GO; GO:0030307; P:positive regulation of cell growth; ISO:MGI.
DR GO; GO:0032754; P:positive regulation of interleukin-5 production; IMP:UniProtKB.
DR GO; GO:0050730; P:regulation of peptidyl-tyrosine phosphorylation; IDA:MGI.
DR GO; GO:0046425; P:regulation of receptor signaling pathway via JAK-STAT; IDA:MGI.
DR InterPro; IPR018049; IL-7/IL-9_CS.
DR InterPro; IPR020447; IL-9.
DR PANTHER; PTHR16926; PTHR16926; 1.
DR PRINTS; PR01926; INTERLEUKIN9.
DR PROSITE; PS00255; INTERLEUKIN_7_9; 1.
PE 1: Evidence at protein level;
KW Cytokine; Direct protein sequencing; Glycoprotein; Growth factor;
KW Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000269|PubMed:2528451"
FT CHAIN 19..144
FT /note="Interleukin-9"
FT /id="PRO_0000015628"
FT MOD_RES 19
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:2528451"
FT CARBOHYD 50
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:2528451"
FT CARBOHYD 78
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:2528451"
FT CARBOHYD 101
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:2528451"
FT CARBOHYD 114
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:2528451"
SQ SEQUENCE 144 AA; 16075 MW; ECD944632CE837C7 CRC64;
MLVTYILASV LLFSSVLGQR CSTTWGIRDT NYLIENLKDD PPSKCSCSGN VTSCLCLSVP
TDDCTTPCYR EGLLQLTNAT QKSRLLPVFH RVKRIVEVLK NITCPSFSCE KPCNQTMAGN
TLSFLKSLLG TFQKTEMQRQ KSRP
