ILA_ARATH
ID ILA_ARATH Reviewed; 2696 AA.
AC F4I893; F4I894; Q9XIR5;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=Protein ILITYHIA {ECO:0000312|EMBL:AEE34290.1};
GN Name=ILA {ECO:0000312|EMBL:AEE34290.1};
GN OrderedLocusNames=At1g64790 {ECO:0000312|Araport:AT1G64790};
GN ORFNames=F13O11.10 {ECO:0000312|EMBL:AAD38254.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION.
RX PubMed=17915010; DOI=10.1186/gb-2007-8-10-r204;
RA Johnston A.J., Meier P., Gheyselinck J., Wuest S.E.J., Federer M.,
RA Schlagenhauf E., Becker J.D., Grossniklaus U.;
RT "Genetic subtraction profiling identifies genes essential for Arabidopsis
RT reproduction and reveals interaction between the female gametophyte and the
RT maternal sporophyte.";
RL Genome Biol. 8:R204.1-R204.21(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1887, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Root;
RX PubMed=18433157; DOI=10.1021/pr8000173;
RA de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E.,
RA Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J.,
RA Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H.;
RT "Site-specific phosphorylation profiling of Arabidopsis proteins by mass
RT spectrometry and peptide chip analysis.";
RL J. Proteome Res. 7:2458-2470(2008).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1887, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1887, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND LACK OF INDUCTION BY PATHOGEN.
RX PubMed=20360018; DOI=10.1093/pcp/pcq038;
RA Monaghan J., Li X.;
RT "The HEAT repeat protein ILITYHIA is required for plant immunity.";
RL Plant Cell Physiol. 51:742-753(2010).
RN [8]
RP FUNCTION.
RX PubMed=21998587; DOI=10.1371/journal.ppat.1002291;
RA Zeng W., Brutus A., Kremer J.M., Withers J.C., Gao X., Jones A.D., He S.Y.;
RT "A genetic screen reveals Arabidopsis stomatal and/or apoplastic defenses
RT against Pseudomonas syringae pv. tomato DC3000.";
RL PLoS Pathog. 7:E1002291-E1002291(2011).
CC -!- FUNCTION: Involved in immunity against bacterial infection and in non-
CC host resistance (PubMed:20360018). Required for embryo development
CC (PubMed:17915010). Required for systemic acquired resistance, but
CC functions in an salicylic acid-independent manner (PubMed:20360018).
CC Required for bacterium-triggered stomatal closure response
CC (PubMed:21998587). {ECO:0000269|PubMed:17915010,
CC ECO:0000269|PubMed:20360018, ECO:0000269|PubMed:21998587}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4I893-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4I893-2; Sequence=VSP_058640, VSP_058641, VSP_058642;
CC -!- INDUCTION: Not up-regulated by pathogen infection.
CC {ECO:0000269|PubMed:20360018}.
CC -!- DISRUPTION PHENOTYPE: Reduced growth, pale or yellow leaves, frequent
CC enations and pollen defect resulting in sterility. Enhanced
CC susceptibility to bacterial infection. {ECO:0000269|PubMed:20360018}.
CC -!- MISCELLANEOUS: This protein was called 'ILITYHIA' after the Greek
CC goddess of childbirth. {ECO:0000305|PubMed:17915010}.
CC -!- MISCELLANEOUS: Unlike other GCN1 homologs, ILA is not a component of
CC the MOS4-associated complex (MSC), a protein complex with homology to
CC the nineteen complex (NTC) in yeast and human.
CC {ECO:0000305|PubMed:20360018}.
CC -!- SIMILARITY: Belongs to the GCN1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD38254.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006193; AAD38254.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34289.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34290.1; -; Genomic_DNA.
DR PIR; B96671; B96671.
DR RefSeq; NP_001185313.1; NM_001198384.1. [F4I893-1]
DR RefSeq; NP_176659.6; NM_105153.8. [F4I893-2]
DR AlphaFoldDB; F4I893; -.
DR STRING; 3702.AT1G64790.2; -.
DR iPTMnet; F4I893; -.
DR PaxDb; F4I893; -.
DR PRIDE; F4I893; -.
DR ProteomicsDB; 250651; -. [F4I893-1]
DR EnsemblPlants; AT1G64790.1; AT1G64790.1; AT1G64790. [F4I893-2]
DR EnsemblPlants; AT1G64790.2; AT1G64790.2; AT1G64790. [F4I893-1]
DR GeneID; 842787; -.
DR Gramene; AT1G64790.1; AT1G64790.1; AT1G64790. [F4I893-2]
DR Gramene; AT1G64790.2; AT1G64790.2; AT1G64790. [F4I893-1]
DR KEGG; ath:AT1G64790; -.
DR Araport; AT1G64790; -.
DR TAIR; locus:2010891; AT1G64790.
DR eggNOG; KOG1242; Eukaryota.
DR HOGENOM; CLU_000504_2_0_1; -.
DR InParanoid; F4I893; -.
DR OMA; EESAWRT; -.
DR OrthoDB; 160593at2759; -.
DR PRO; PR:F4I893; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I893; baseline and differential.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0019901; F:protein kinase binding; IEA:InterPro.
DR GO; GO:0019887; F:protein kinase regulator activity; IBA:GO_Central.
DR GO; GO:0043022; F:ribosome binding; IEA:InterPro.
DR GO; GO:0034198; P:cellular response to amino acid starvation; IBA:GO_Central.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0009682; P:induced systemic resistance; IMP:UniProtKB.
DR GO; GO:0045087; P:innate immune response; IMP:UniProtKB.
DR GO; GO:0033674; P:positive regulation of kinase activity; IEA:InterPro.
DR GO; GO:0006417; P:regulation of translation; IBA:GO_Central.
DR GO; GO:0009627; P:systemic acquired resistance; IMP:TAIR.
DR Gene3D; 1.25.10.10; -; 8.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033173; Gcn1.
DR InterPro; IPR021133; HEAT_type_2.
DR InterPro; IPR034085; TOG.
DR PANTHER; PTHR23346:SF7; PTHR23346:SF7; 1.
DR SMART; SM01349; TOG; 3.
DR SUPFAM; SSF48371; SSF48371; 4.
DR PROSITE; PS50077; HEAT_REPEAT; 3.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..2696
FT /note="Protein ILITYHIA"
FT /id="PRO_0000438304"
FT REPEAT 162..204
FT /note="HEAT 1"
FT /evidence="ECO:0000255"
FT REPEAT 253..290
FT /note="HEAT 2"
FT /evidence="ECO:0000255"
FT REPEAT 303..340
FT /note="HEAT 3"
FT /evidence="ECO:0000255"
FT REPEAT 364..400
FT /note="HEAT 4"
FT /evidence="ECO:0000255"
FT REPEAT 401..438
FT /note="HEAT 5"
FT /evidence="ECO:0000255"
FT REPEAT 487..525
FT /note="HEAT 6"
FT /evidence="ECO:0000255"
FT REPEAT 526..562
FT /note="HEAT 7"
FT /evidence="ECO:0000255"
FT REPEAT 564..601
FT /note="HEAT 8"
FT /evidence="ECO:0000255"
FT REPEAT 642..677
FT /note="HEAT 9"
FT /evidence="ECO:0000255"
FT REPEAT 985..1021
FT /note="HEAT 10"
FT /evidence="ECO:0000255"
FT REPEAT 1082..1118
FT /note="HEAT 11"
FT /evidence="ECO:0000255"
FT REPEAT 1188..1225
FT /note="HEAT 12"
FT /evidence="ECO:0000255"
FT REPEAT 1273..1311
FT /note="HEAT 13"
FT /evidence="ECO:0000255"
FT REPEAT 1315..1355
FT /note="HEAT 14"
FT /evidence="ECO:0000255"
FT REPEAT 1358..1395
FT /note="HEAT 15"
FT /evidence="ECO:0000255"
FT REPEAT 1397..1433
FT /note="HEAT 16"
FT /evidence="ECO:0000255"
FT REPEAT 1436..1474
FT /note="HEAT 17"
FT /evidence="ECO:0000255"
FT REPEAT 1478..1515
FT /note="HEAT 18"
FT /evidence="ECO:0000255"
FT REPEAT 1516..1553
FT /note="HEAT 19"
FT /evidence="ECO:0000255"
FT REPEAT 1564..1600
FT /note="HEAT 20"
FT /evidence="ECO:0000255"
FT REPEAT 1601..1638
FT /note="HEAT 21"
FT /evidence="ECO:0000255"
FT REPEAT 1640..1677
FT /note="HEAT 22"
FT /evidence="ECO:0000255"
FT REPEAT 1683..1720
FT /note="HEAT 23"
FT /evidence="ECO:0000255"
FT REPEAT 1722..1759
FT /note="HEAT 24"
FT /evidence="ECO:0000255"
FT REPEAT 1761..1797
FT /note="HEAT 25"
FT /evidence="ECO:0000255"
FT REPEAT 1801..1838
FT /note="HEAT 26"
FT /evidence="ECO:0000255"
FT REPEAT 1840..1876
FT /note="HEAT 27"
FT /evidence="ECO:0000255"
FT REPEAT 1908..1945
FT /note="HEAT 28"
FT /evidence="ECO:0000255"
FT REPEAT 1949..1986
FT /note="HEAT 29"
FT /evidence="ECO:0000255"
FT REPEAT 1988..2024
FT /note="HEAT 30"
FT /evidence="ECO:0000255"
FT REPEAT 2029..2066
FT /note="HEAT 31"
FT /evidence="ECO:0000255"
FT REPEAT 2067..2102
FT /note="HEAT 32"
FT /evidence="ECO:0000255"
FT REPEAT 2104..2137
FT /note="HEAT 33"
FT /evidence="ECO:0000255"
FT REPEAT 2138..2175
FT /note="HEAT 34"
FT /evidence="ECO:0000255"
FT REPEAT 2177..2213
FT /note="HEAT 35"
FT /evidence="ECO:0000255"
FT REPEAT 2217..2254
FT /note="HEAT 36"
FT /evidence="ECO:0000255"
FT REPEAT 2258..2292
FT /note="HEAT 37"
FT /evidence="ECO:0000255"
FT REPEAT 2293..2330
FT /note="HEAT 38"
FT /evidence="ECO:0000255"
FT REPEAT 2335..2373
FT /note="HEAT 39"
FT /evidence="ECO:0000255"
FT REPEAT 2377..2414
FT /note="HEAT 40"
FT /evidence="ECO:0000255"
FT REPEAT 2416..2450
FT /note="HEAT 41"
FT /evidence="ECO:0000255"
FT REPEAT 2455..2492
FT /note="HEAT 42"
FT /evidence="ECO:0000255"
FT REPEAT 2494..2530
FT /note="HEAT 43"
FT /evidence="ECO:0000255"
FT REPEAT 2536..2573
FT /note="HEAT 44"
FT /evidence="ECO:0000255"
FT REPEAT 2580..2617
FT /note="HEAT 45"
FT /evidence="ECO:0000255"
FT REPEAT 2620..2658
FT /note="HEAT 46"
FT /evidence="ECO:0000255"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 901..941
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 901..916
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 918..941
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1887
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18433157,
FT ECO:0007744|PubMed:19245862, ECO:0007744|PubMed:19376835"
FT VAR_SEQ 1..119
FT /note="Missing (in isoform 2)"
FT /id="VSP_058640"
FT VAR_SEQ 1089
FT /note="P -> PILERILLSSKRTKLHDDVLQILYMHLDPMLPLPRLRMIS (in
FT isoform 2)"
FT /id="VSP_058641"
FT VAR_SEQ 1569..1574
FT /note="Missing (in isoform 2)"
FT /id="VSP_058642"
SQ SEQUENCE 2696 AA; 294075 MW; 6BAD94E9B6FEC1B3 CRC64;
MSYSMVNASS AVSSPETAKN SDEPPPISSE AVNVLFPSVD PNSKLFRNSL NITISREAPP
LTTSRIDFLS LFIFCKLTHW LSLNPSSHRD EEEEEASPFY PFTIVLTYQP GPGQSPWKEM
ASPLESLLSI SGSVSTSSTL IRLRIFRHDI PEILQNSDMT SDIAPVIVDM IFQTLAIYDD
RASRKAVDDL IVKGLGNVTF MKTFAAMLVQ VMEKQLKFCF DTVCYRLLIW SCLLLEKSQF
ATVSKNAFVR VASTQASLLR IIMESSFRMR RACKRFMFHL FSQSQAIYSL YMDEVKGSRI
PYKDSPELLG LLLEFSCSSP ALFEQSKAIF VDIYVKDVLN SREKQKPNLS NCFKPLLQRL
SHEEFQTVIL PAAVKMLKRN PEIVLESVGF LLANVNIDLS KYALELLPVI LPQARHTDED
RRLGALSMVM CLSEKSSNPD TIEAMFASVK AIIGGSEGRL QSPHQRIGML NAVQELASAP
EGKYIGSLSR TICSFLIACY KDEGNEDVKL SILSAVASWA SRSSVAIQPN LVSFIAAGLK
EKEALRRGHL RCVRIICRNP DTISQISDLL SPLIQLVKTG FTKAVQRLDG IYALLIVSKI
AACDIKAEDT MVKEKLWTLI SQNEPSLVQI TLASKLSSDD CVVCVDLLEV LLVEHSSRVL
EAFSLKSLSQ LLLFLLCHPS WNVRKTAYNS VTKIFLATSQ LATTLLDEFS DFLSITGDQI
VSSRTSDADN PADHQAPFVP SVEVLVKALI VISSAAVAGP PSSWIVRAIF CSHHPSIVGT
GKRDAVWKRL QKCLKTCGFD VATFLSTNGE SVCKSLLGPM GLTSAKTPEQ QAAVYSLSTM
MSLAPEDTFT VFKMHLQDLP DRLSHDMLSE TDIKIFHTPE GMLLSEQGVY VAQTIGAKYT
KQEPSSNHSL KKGLASRETA NSGRRDTAKL TKKADKGKTA KEEARELMLK EEASTRENVH
RIQKSLSLVL HALGEMGLAN PVFCHSQLPF LATFLDPLLR SPIVSAAAFE NLVKLARCTV
QPLCNWALEI STALRLIAID EVDTSFDFRP SVDKAGKTYE GLFERIVNGL SISCKSGPLP
VDTFTFIFPV LYHVLGVVPA YQASVGPALN ELCLGLQADD VANALYGVYS KDVHVRLACL
NAVKCIPAVS KCSLPQNVKI ATNIWIALHD PEKSVAESAD DLWARYGHDL GTDYSGIFKA
LSHINLNVRL AAAEALADAL HESPSSIQLS LSTLFSLYIR DATSGEDVFD AGWIGRQGIA
LALQSAADVL TTKDLPAVMT FLISRALADP NTDVRGKMIN AGIMIIDKHG KENVSLLFPI
FENYLNKEAS DEEEYDLVRE GVVIFTGALA KHLARDDPKV HNVVEKLLEV LNTPSESVQR
AVSTCLSPLV LSKQEEAPAL FLRLLDKLMK SDKYGERRGA AFGLAGVVMG FGISSLKKYG
LIVTLQEALI DRNSAKRREG ALLAFECLCE KLGKLFEPYV IKMLPLLLVS FSDQVGAVRE
AAECAARAMM SQLSAYGVKL VLPSLLKGLE DKAWRTKQSS VQLLGAMAFC APQQLSQCLP
RVVPKLTEVF KTIQVLTDTH PKVQSAGQLA LQQVGSVIKN PEISSLVPTL LLALTDPNEY
TRHALDTLLQ TTFVNSVDAP SLALLVPIVH RGLRERSSET KKKASQIVGN MCSLVTEPKD
MIPYIGLLLP EVKKVLVDPI PEVRSVAARA VGSLIRGMGE DNFPDLVPWL FETLKSDTSN
VERYGAAQGL SEVIAALGTD YFENILPDLI RHCSHQKASV RDGYLTLFKF LPRSLGAQFQ
KYLQLVLPAI LDGLADENES VRDAALGAGH VLVEHHATTS LPLLLPAVED GIFNDNWRIR
QSSVELLGDL LFKVAGTSGK ALLEGGSDDE GASTEAQGRA IIDILGMDKR NEVLAALYMV
RTDVSLSVRQ AALHVWKTIV ANTPKTLKEI MPILMSTLIS SLASPSSERR QVAGRSLGEL
VRKLGERVLP LIIPILSKGL KDPDVDKRQG VCIGLNEVMA SAGRSQLLSF MDQLIPTIRT
ALCDSALEVR ESAGLAFSTL YKSAGLQAMD EIIPTLLEAL EDDEMSTTAL DGLKQIISVR
TAAVLPHILP KLVHLPLSAL NAHALGALAE VAGAGFNTHL GTILPALLSA MGGENKEVQE
LAQEAAERVV LVIDEEGVET LLSELLKGVS DSQASIRRSS AYLIGYFFKS SKLYLIDEAP
NMISTLIVML SDSDSTTVAV SWEALARVIG SVPKEVLPSY IKLVRDAVST ARDKERRKRK
GGYVVIPGLC LPKSLKPLLP VFLQGLISGS AELREQAAIG LGELIEVTSE QALKEFVIPI
TGPLIRIIGD RFPWQVKSAI LATLIILIQR GGMALKPFLP QLQTTFVKCL QDSTRTIRSS
AAVALGKLSA LSTRIDPLVG DLMTSFQAAD SGVREAILSA MRGVIKHAGK SIGPAVRVRI
FDLLKDLMHH EDDQVRISAT SMLGVLSQYL EAAQLSVLLQ EVNDLSASQN WGARHGSVLC
ISSLLKHNPS TIMTSSLFSS MLNSLKSSLK DEKFPLRESS TKALGRLLLK QLATDPSNTK
VVIDVLSSIV SALHDDSSEV RRRALSSLKA FAKDNPSATM ANISVIGPPL AECLKDGNTP
VRLAAERCAL HVFQLTKGAE NVQAAQKYIT GLDARRLSKF PEQSDDSESD DDNVSG
