ILEUC_MOUSE
ID ILEUC_MOUSE Reviewed; 375 AA.
AC Q5SV42; Q5SW82; Q8K3Y1;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Leukocyte elastase inhibitor C;
DE AltName: Full=Serine protease inhibitor EIC;
DE AltName: Full=Serpin B1c;
GN Name=Serpinb1c;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=129S6/SvEvTac; TISSUE=Spleen;
RX PubMed=12189154; DOI=10.1074/jbc.m207080200;
RA Benarafa C., Cooley J., Zeng W., Bird P.I., Remold-O'Donnell E.;
RT "Characterization of four murine homologs of the human ov-serpin monocyte
RT neutrophil elastase inhibitor MNEI (SERPINB1).";
RL J. Biol. Chem. 277:42028-42033(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, AND INTERACTION WITH CASP1 AND CASP4.
RX PubMed=30692621; DOI=10.1038/s41590-018-0303-z;
RA Choi Y.J., Kim S., Choi Y., Nielsen T.B., Yan J., Lu A., Ruan J., Lee H.R.,
RA Wu H., Spellberg B., Jung J.U.;
RT "SERPINB1-mediated checkpoint of inflammatory caspase activation.";
RL Nat. Immunol. 20:276-287(2019).
CC -!- FUNCTION: Regulates the activity of the neutrophil proteases. Forms
CC only a stable complex with CTSG/Cathepsin G (By similarity). During
CC inflammation, limits the activity of inflammatory caspases CASP1 and
CC CASP4 by suppressing their caspase-recruitment domain (CARD)
CC oligomerization and enzymatic activation (PubMed:30692621).
CC {ECO:0000250|UniProtKB:Q8VHP7, ECO:0000269|PubMed:30692621}.
CC -!- SUBUNIT: Monomer. Interacts (via C-terminus) with CASP1 and CASP4 (via
CC CARD domain); these interactions regulate the activity of inflammatory
CC caspases. {ECO:0000269|PubMed:30692621}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in heart. {ECO:0000269|PubMed:12189154}.
CC -!- SIMILARITY: Belongs to the serpin family. Ov-serpin subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAI25076.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF521699; AAM95935.1; -; Genomic_DNA.
DR EMBL; AL645693; CAI24600.1; -; Genomic_DNA.
DR EMBL; AL606533; CAI24600.1; JOINED; Genomic_DNA.
DR EMBL; AL606533; CAI25076.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL606533; CAI25077.1; -; Genomic_DNA.
DR EMBL; AL645693; CAI25077.1; JOINED; Genomic_DNA.
DR EMBL; BC104332; AAI04333.1; -; mRNA.
DR CCDS; CCDS26429.1; -.
DR RefSeq; NP_766639.2; NM_173051.2.
DR AlphaFoldDB; Q5SV42; -.
DR SMR; Q5SV42; -.
DR STRING; 10090.ENSMUSP00000021834; -.
DR MEROPS; I04.006; -.
DR iPTMnet; Q5SV42; -.
DR PhosphoSitePlus; Q5SV42; -.
DR jPOST; Q5SV42; -.
DR MaxQB; Q5SV42; -.
DR PaxDb; Q5SV42; -.
DR PeptideAtlas; Q5SV42; -.
DR PRIDE; Q5SV42; -.
DR DNASU; 380839; -.
DR Ensembl; ENSMUST00000021834; ENSMUSP00000021834; ENSMUSG00000079049.
DR GeneID; 380839; -.
DR KEGG; mmu:380839; -.
DR UCSC; uc007pzw.2; mouse.
DR CTD; 380839; -.
DR MGI; MGI:2445363; Serpinb1c.
DR VEuPathDB; HostDB:ENSMUSG00000079049; -.
DR eggNOG; KOG2392; Eukaryota.
DR GeneTree; ENSGT00940000154573; -.
DR HOGENOM; CLU_023330_0_2_1; -.
DR InParanoid; Q5SV42; -.
DR OMA; CMAIEED; -.
DR OrthoDB; 1124079at2759; -.
DR PhylomeDB; Q5SV42; -.
DR TreeFam; TF352619; -.
DR BioGRID-ORCS; 380839; 1 hit in 70 CRISPR screens.
DR ChiTaRS; Serpinb1c; mouse.
DR PRO; PR:Q5SV42; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q5SV42; protein.
DR Bgee; ENSMUSG00000079049; Expressed in animal zygote and 20 other tissues.
DR ExpressionAtlas; Q5SV42; baseline and differential.
DR GO; GO:0036464; C:cytoplasmic ribonucleoprotein granule; ISO:MGI.
DR GO; GO:0005576; C:extracellular region; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0030414; F:peptidase inhibitor activity; ISO:MGI.
DR GO; GO:0010951; P:negative regulation of endopeptidase activity; ISO:MGI.
DR GO; GO:0032691; P:negative regulation of interleukin-1 beta production; IMP:UniProtKB.
DR GO; GO:0044342; P:type B pancreatic cell proliferation; ISO:MGI.
DR Gene3D; 2.30.39.10; -; 1.
DR Gene3D; 3.30.497.10; -; 1.
DR InterPro; IPR015557; Serpin_B1.
DR InterPro; IPR023795; Serpin_CS.
DR InterPro; IPR023796; Serpin_dom.
DR InterPro; IPR000215; Serpin_fam.
DR InterPro; IPR036186; Serpin_sf.
DR InterPro; IPR042178; Serpin_sf_1.
DR InterPro; IPR042185; Serpin_sf_2.
DR PANTHER; PTHR11461; PTHR11461; 1.
DR PANTHER; PTHR11461:SF180; PTHR11461:SF180; 1.
DR Pfam; PF00079; Serpin; 1.
DR SMART; SM00093; SERPIN; 1.
DR SUPFAM; SSF56574; SSF56574; 1.
DR PROSITE; PS00284; SERPIN; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Protease inhibitor; Reference proteome;
KW Serine protease inhibitor.
FT CHAIN 1..375
FT /note="Leukocyte elastase inhibitor C"
FT /id="PRO_0000289122"
FT SITE 343..344
FT /note="Reactive bond"
FT /evidence="ECO:0000250"
FT CONFLICT 293
FT /note="L -> P (in Ref. 1; AAM95935)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 375 AA; 41920 MW; 71F3DB94E10FAC67 CRC64;
MGQLSSANNL FALELFHTLN ESNPTGNTIF SPVSISSALA MVYLGARGST AAQLSKTLHF
DSAEDIHSQF QSLTAEVSKR GASHTLKLAN RLYGEKTYNF LPEYLASIQK TYSADLALVD
FQHASEDARK EINQWVKGQT EEKIQELFAV GVVDSMTKLV LVNATYFKGM WQKKFMARDT
TDAPFRLSKK VTKTVKMMYL KNNLPFGYIP DLKCKVLEMP YQGGELSMVI LLPEDIEDET
TGLEEIEKQL TLEKLQECEN LQNIDVCVKL PKFKMEESYI LNSNLGQLGV QDLFSSSKAD
LSGMSGSRDL FISKIVHKSY VEVNEEGTET DAAMPGTVVG CCLMPMEFTV DHPFLFFIRH
NPTAHVLFLG RVCSP
