ILK_BOVIN
ID ILK_BOVIN Reviewed; 452 AA.
AC Q3SWY2;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Integrin-linked protein kinase {ECO:0000250|UniProtKB:Q13418};
DE EC=2.7.11.1 {ECO:0000250|UniProtKB:Q13418};
DE AltName: Full=59 kDa serine/threonine-protein kinase {ECO:0000250|UniProtKB:Q13418};
DE AltName: Full=Beta-integrin-linked kinase {ECO:0000250|UniProtKB:P57044};
DE AltName: Full=ILK-1 {ECO:0000250|UniProtKB:Q13418};
DE AltName: Full=ILK-2 {ECO:0000250|UniProtKB:Q13418};
DE AltName: Full=p59ILK {ECO:0000250|UniProtKB:Q13418};
GN Name=ILK {ECO:0000250|UniProtKB:Q13418};
GN Synonyms=ILK1 {ECO:0000250|UniProtKB:Q13418},
GN ILK2 {ECO:0000250|UniProtKB:Q13418};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Ascending colon;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Receptor-proximal protein kinase regulating integrin-mediated
CC signal transduction. May act as a mediator of inside-out integrin
CC signaling. Focal adhesion protein part of the complex ILK-PINCH. This
CC complex is considered to be one of the convergence points of
CC integrin- and growth factor-signaling pathway. Could be implicated in
CC mediating cell architecture, adhesion to integrin substrates and
CC anchorage-dependent growth in epithelial cells. Regulates cell motility
CC by forming a complex with PARVB. Phosphorylates beta-1 and beta-3
CC integrin subunit on serine and threonine residues, but also AKT1 and
CC GSK3B. {ECO:0000250|UniProtKB:Q13418}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000250|UniProtKB:Q13418};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1; Evidence={ECO:0000250|UniProtKB:Q13418};
CC -!- ACTIVITY REGULATION: Stimulated rapidly but transiently by both cell
CC fibronectin interactions, as well as by insulin, in a PI3-K-dependent
CC manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain
CC of ILK. The protein kinase activity is stimulated by LIMD2.
CC {ECO:0000250|UniProtKB:Q13418}.
CC -!- SUBUNIT: Interacts with the cytoplasmic domain of ITGB1. Could also
CC interact with integrin ITGB2, ITGB3 and/or ITGB5. Interacts (via ANK
CC repeats) with LIMS1 and LIMS2. Interacts with PARVA (via C-terminus)
CC and PARVB; these compete for the same binding site (By similarity).
CC Interacts probably also with TGFB1I1 (By similarity). Interacts (via
CC ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but
CC independent of the kinase activity of EPHA1 (By similarity). Interacts
CC with FERMT2 (By similarity). Interacts with LIMD2; leading to activate
CC the protein kinase activity. Interacts with PXN/PAXILLIN (via LD motif
CC 4). Interacts with CCDC25 (via cytoplasmic region); initiating the ILK-
CC PARVB cascade to induce cytoskeleton rearrangement and directional
CC migration of cells (By similarity). {ECO:0000250|UniProtKB:O55222,
CC ECO:0000250|UniProtKB:Q13418}.
CC -!- SUBCELLULAR LOCATION: Cell junction, focal adhesion
CC {ECO:0000250|UniProtKB:Q13418}. Cell membrane
CC {ECO:0000250|UniProtKB:Q13418}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q13418}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:Q13418}. Cell projection, lamellipodium
CC {ECO:0000250|UniProtKB:O55222}. Cytoplasm, myofibril, sarcomere
CC {ECO:0000250|UniProtKB:Q13418}.
CC -!- DOMAIN: A PH-like domain is involved in phosphatidylinositol phosphate
CC binding. {ECO:0000250|UniProtKB:Q13418}.
CC -!- PTM: Autophosphorylated on serine residues.
CC {ECO:0000250|UniProtKB:Q13418}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr
CC protein kinase family. {ECO:0000305}.
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DR EMBL; BC104603; AAI04604.1; -; mRNA.
DR RefSeq; NP_001029865.1; NM_001034693.1.
DR RefSeq; XP_005216186.1; XM_005216129.3.
DR RefSeq; XP_005216187.1; XM_005216130.2.
DR RefSeq; XP_005216188.1; XM_005216131.1.
DR AlphaFoldDB; Q3SWY2; -.
DR BMRB; Q3SWY2; -.
DR SMR; Q3SWY2; -.
DR STRING; 9913.ENSBTAP00000000927; -.
DR PaxDb; Q3SWY2; -.
DR PeptideAtlas; Q3SWY2; -.
DR PRIDE; Q3SWY2; -.
DR Ensembl; ENSBTAT00000000927; ENSBTAP00000000927; ENSBTAG00000000696.
DR GeneID; 540207; -.
DR KEGG; bta:540207; -.
DR CTD; 3611; -.
DR VEuPathDB; HostDB:ENSBTAG00000000696; -.
DR VGNC; VGNC:30177; ILK.
DR eggNOG; KOG0195; Eukaryota.
DR GeneTree; ENSGT00940000155956; -.
DR InParanoid; Q3SWY2; -.
DR OMA; NMADAKF; -.
DR Proteomes; UP000009136; Chromosome 15.
DR Bgee; ENSBTAG00000000696; Expressed in myometrium and 108 other tissues.
DR GO; GO:0005925; C:focal adhesion; IBA:GO_Central.
DR GO; GO:0030027; C:lamellipodium; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030017; C:sarcomere; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0019901; F:protein kinase binding; IEA:Ensembl.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0001658; P:branching involved in ureteric bud morphogenesis; IEA:Ensembl.
DR GO; GO:0030030; P:cell projection organization; IEA:Ensembl.
DR GO; GO:0007160; P:cell-matrix adhesion; IBA:GO_Central.
DR GO; GO:0045197; P:establishment or maintenance of epithelial cell apical/basal polarity; IEA:Ensembl.
DR GO; GO:0010761; P:fibroblast migration; IEA:Ensembl.
DR GO; GO:0007229; P:integrin-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0022011; P:myelination in peripheral nervous system; IEA:Ensembl.
DR GO; GO:2000178; P:negative regulation of neural precursor cell proliferation; IEA:Ensembl.
DR GO; GO:0021675; P:nerve development; IEA:Ensembl.
DR GO; GO:0061351; P:neural precursor cell proliferation; IEA:Ensembl.
DR GO; GO:0003151; P:outflow tract morphogenesis; IEA:Ensembl.
DR GO; GO:0018105; P:peptidyl-serine phosphorylation; IEA:Ensembl.
DR GO; GO:0030513; P:positive regulation of BMP signaling pathway; IEA:Ensembl.
DR GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; IEA:Ensembl.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IEA:Ensembl.
DR GO; GO:1901224; P:positive regulation of NIK/NF-kappaB signaling; IEA:Ensembl.
DR GO; GO:0045669; P:positive regulation of osteoblast differentiation; IEA:Ensembl.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; IEA:Ensembl.
DR GO; GO:1900026; P:positive regulation of substrate adhesion-dependent cell spreading; IEA:Ensembl.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:Ensembl.
DR GO; GO:0043491; P:protein kinase B signaling; IEA:Ensembl.
DR GO; GO:0034446; P:substrate adhesion-dependent cell spreading; IBA:GO_Central.
DR GO; GO:0033209; P:tumor necrosis factor-mediated signaling pathway; IEA:Ensembl.
DR CDD; cd14057; PK_ILK; 1.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR035692; PK_ILK.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR Pfam; PF12796; Ank_2; 2.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR SMART; SM00248; ANK; 3.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 3.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE 2: Evidence at transcript level;
KW Acetylation; ANK repeat; ATP-binding; Cell junction; Cell membrane;
KW Cell projection; Cytoplasm; Kinase; Membrane; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Repeat;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..452
FT /note="Integrin-linked protein kinase"
FT /id="PRO_0000244820"
FT REPEAT 2..30
FT /note="ANK 1"
FT REPEAT 31..63
FT /note="ANK 2"
FT REPEAT 64..96
FT /note="ANK 3"
FT REPEAT 97..129
FT /note="ANK 4"
FT REPEAT 130..174
FT /note="ANK 5"
FT DOMAIN 193..446
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 33..139
FT /note="Interaction with LIMS1"
FT /evidence="ECO:0000250"
FT REGION 180..212
FT /note="PH-like; mediates interaction with TGFB1I1"
FT BINDING 199..207
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 220
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q13418"
FT MOD_RES 186
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q13418"
FT MOD_RES 426
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:O55222"
SQ SEQUENCE 452 AA; 51447 MW; E60EC47224C170C5 CRC64;
MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV EMLIMRGARI
NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG NVPLHYACFW GQDQVAEDLV
ANGALVSICN KYGEMPVDKA KPPLRELLRE RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG
TLNKHSGIDF KQLNFLAKLN ENHSGELWKG RWQGNDIVVK MLKVRDWSTR KSRDFNEECP
RLRIFSHPNV LPVLGACQSP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD
MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM YAPAWVAPEA
LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI GMKVALEGLR PTIPPGISPH
VCKLMKICMN EDPAKRPKFD MIVPILEKMQ DK
