ID   ILL2_ORYSJ              Reviewed;         456 AA.
AC   Q5N8F2; A0A0P0V751;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=IAA-amino acid hydrolase ILR1-like 2;
DE            EC=3.5.1.-;
DE   Flags: Precursor;
GN   Name=ILL2; OrderedLocusNames=Os01g0706900, LOC_Os01g51060;
GN   ORFNames=P0510F09.6, P0692C11.28;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA   Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA   Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA   Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA   Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA   Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA   Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA   Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA   Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA   Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA   Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Hydrolyzes certain amino acid conjugates of the plant growth
CC       regulator indole-3-acetic acid (IAA). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen {ECO:0000255|PROSITE-
CC       ProRule:PRU10138}.
CC   -!- SIMILARITY: Belongs to the peptidase M20 family. {ECO:0000305}.
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DR   EMBL; AP003273; BAD81927.1; -; Genomic_DNA.
DR   EMBL; AP003381; BAD82256.1; -; Genomic_DNA.
DR   EMBL; AP008207; BAF05934.1; -; Genomic_DNA.
DR   EMBL; AP014957; BAS73948.1; -; Genomic_DNA.
DR   EMBL; AK105646; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015624333.1; XM_015768847.1.
DR   AlphaFoldDB; Q5N8F2; -.
DR   SMR; Q5N8F2; -.
DR   STRING; 4530.OS01T0706900-01; -.
DR   MEROPS; M20.014; -.
DR   PaxDb; Q5N8F2; -.
DR   PRIDE; Q5N8F2; -.
DR   EnsemblPlants; Os01t0706900-01; Os01t0706900-01; Os01g0706900.
DR   GeneID; 4327677; -.
DR   Gramene; Os01t0706900-01; Os01t0706900-01; Os01g0706900.
DR   KEGG; osa:4327677; -.
DR   eggNOG; ENOG502QQEM; Eukaryota.
DR   HOGENOM; CLU_023257_0_0_1; -.
DR   InParanoid; Q5N8F2; -.
DR   OMA; MKMHACG; -.
DR   OrthoDB; 455218at2759; -.
DR   Proteomes; UP000000763; Chromosome 1.
DR   Proteomes; UP000059680; Chromosome 1.
DR   Genevisible; Q5N8F2; OS.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR   GO; GO:0016787; F:hydrolase activity; IBA:GO_Central.
DR   GO; GO:0010179; F:IAA-Ala conjugate hydrolase activity; IBA:GO_Central.
DR   GO; GO:0009850; P:auxin metabolic process; IBA:GO_Central.
DR   CDD; cd08017; M20_IAA_Hyd; 1.
DR   InterPro; IPR017439; Amidohydrolase.
DR   InterPro; IPR036264; Bact_exopeptidase_dim_dom.
DR   InterPro; IPR044757; ILR1-like_Hyd.
DR   InterPro; IPR002933; Peptidase_M20.
DR   InterPro; IPR011650; Peptidase_M20_dimer.
DR   PANTHER; PTHR11014; PTHR11014; 1.
DR   Pfam; PF07687; M20_dimer; 1.
DR   Pfam; PF01546; Peptidase_M20; 1.
DR   PIRSF; PIRSF005962; Pept_M20D_amidohydro; 1.
DR   SUPFAM; SSF55031; SSF55031; 1.
DR   TIGRFAMs; TIGR01891; amidohydrolases; 1.
DR   PROSITE; PS00014; ER_TARGET; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Hydrolase; Reference proteome; Signal.
FT   SIGNAL          1..42
FT                   /evidence="ECO:0000255"
FT   CHAIN           43..456
FT                   /note="IAA-amino acid hydrolase ILR1-like 2"
FT                   /id="PRO_0000351638"
FT   MOTIF           453..456
FT                   /note="Prevents secretion from ER"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10138"
FT   CONFLICT        318
FT                   /note="R -> W (in Ref. 5; AK105646)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   456 AA;  48203 MW;  12E45927A0E30FB9 CRC64;
     MARARASAMG AAPPPHLRSH DLVVVVAVAA AALCLACRGA SAGGGGGVDV LDRARRPEFA
     AWMAGVRWAI HERPELAFEE IETSRLVRAE LDAMGVAYRH PVAGTGVVAT VGTGRPPFVA
     LRADMDALPM QEEVQWEHKS KVAMKMHACG HDAHTTMLLG AARILQERRH ELQGTVVLLF
     QPGEEVGTGA RRMVEAGAVD NVEAIFGFHV SVELPTGVVG SRPGPLLAGC GFFEAVITGK
     GGHAAHPHAS VDPILAASTV VLALQGLVSR EADPLEAQVV TVTRFLAGDA LNVIPESITI
     GGTFRVFSNE GFLRLKRRIE EVIVAQSAVY RCAAAVDFHA GGRPLLPPTI NSAALHAHFQ
     AVAAETLGAS AAVLGAMEPC MGSEDFAVFS EAVPASHFYF VGVRNEAEGL VHLAHSPHFR
     VDDAALPYGA ALHASLAMRY LDERRREGGS HPHEEL