ILP1_ARATH
ID ILP1_ARATH Reviewed; 908 AA.
AC Q9FNN3;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Transcriptional repressor ILP1 {ECO:0000303|PubMed:17012601};
DE AltName: Full=INCREASED LEVEL OF POLYPLOIDY 1 {ECO:0000303|PubMed:17012601};
GN Name=ILP1 {ECO:0000303|PubMed:17012601};
GN OrderedLocusNames=At5g08550 {ECO:0000312|Araport:AT5G08550};
GN ORFNames=MAH20.11 {ECO:0000312|EMBL:BAB10004.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, DOMAIN, AND DEVELOPMENTAL STAGE.
RX PubMed=17012601; DOI=10.1105/tpc.106.043869;
RA Yoshizumi T., Tsumoto Y., Takiguchi T., Nagata N., Yamamoto Y.Y.,
RA Kawashima M., Ichikawa T., Nakazawa M., Yamamoto N., Matsui M.;
RT "Increased level of polyploidy1, a conserved repressor of CYCLINA2
RT transcription, controls endoreduplication in Arabidopsis.";
RL Plant Cell 18:2452-2468(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, AND INTERACTION WITH STIPL1.
RX PubMed=25568310; DOI=10.15252/embj.201489478;
RA Dolata J., Guo Y., Kolowerzo A., Smolinski D., Brzyzek G., Jarmolowski A.,
RA Swiezewski S.;
RT "NTR1 is required for transcription elongation checkpoints at alternative
RT exons in Arabidopsis.";
RL EMBO J. 34:544-558(2015).
CC -!- FUNCTION: Transcriptional repressor regulating endoreduplication
CC through control of A-type cyclins expression (PubMed:17012601). Does
CC not bind to promoter sequences (in vitro) and may act by interacting
CC with tissue-specific transcription factors (PubMed:17012601). Enhances
CC the endocycle in endoreduplicating cells in seedlings
CC (PubMed:17012601). Required for efficient splicing (PubMed:25568310).
CC {ECO:0000269|PubMed:17012601, ECO:0000269|PubMed:25568310}.
CC -!- SUBUNIT: Interacts with STIPL1/NTR1 (PubMed:25568310).
CC {ECO:0000269|PubMed:25568310}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17012601}.
CC -!- DEVELOPMENTAL STAGE: Gradual reduction of expression as the first leaf
CC develops. 90% reduction of expression at day 20 after imbibition.
CC {ECO:0000269|PubMed:17012601}.
CC -!- DOMAIN: The C-terminal region (474-908) is responsible for the
CC repressor activity. {ECO:0000269|PubMed:17012601}.
CC -!- DISRUPTION PHENOTYPE: Reduction of polyploidy and inhibition of
CC hypocotyl elongation. {ECO:0000269|PubMed:17012601}.
CC -!- SIMILARITY: Belongs to the GCF family. {ECO:0000305}.
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DR EMBL; AB253763; BAF36503.1; -; mRNA.
DR EMBL; AB006697; BAB10004.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91319.1; -; Genomic_DNA.
DR RefSeq; NP_196472.1; NM_120941.2.
DR AlphaFoldDB; Q9FNN3; -.
DR SMR; Q9FNN3; -.
DR STRING; 3702.AT5G08550.1; -.
DR PaxDb; Q9FNN3; -.
DR PRIDE; Q9FNN3; -.
DR ProteomicsDB; 228863; -.
DR EnsemblPlants; AT5G08550.1; AT5G08550.1; AT5G08550.
DR GeneID; 830755; -.
DR Gramene; AT5G08550.1; AT5G08550.1; AT5G08550.
DR KEGG; ath:AT5G08550; -.
DR Araport; AT5G08550; -.
DR TAIR; locus:2159552; AT5G08550.
DR eggNOG; KOG2136; Eukaryota.
DR HOGENOM; CLU_011441_0_0_1; -.
DR InParanoid; Q9FNN3; -.
DR OMA; STQRTKN; -.
DR OrthoDB; 1217358at2759; -.
DR PhylomeDB; Q9FNN3; -.
DR PRO; PR:Q9FNN3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FNN3; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0030371; F:translation repressor activity; IDA:TAIR.
DR GO; GO:0042023; P:DNA endoreduplication; IMP:TAIR.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IMP:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:TAIR.
DR InterPro; IPR012890; GCFC2-like.
DR InterPro; IPR022783; GCFC_dom.
DR PANTHER; PTHR12214; PTHR12214; 1.
DR Pfam; PF07842; GCFC; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..908
FT /note="Transcriptional repressor ILP1"
FT /id="PRO_0000437690"
FT REGION 1..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 238..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 513..574
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 426..453
FT /evidence="ECO:0000255"
FT COMPBIAS 7..21
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 26..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 65..81
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 241..255
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 513..546
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 558..574
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 908 AA; 100775 MW; 7605175F7B11E134 CRC64;
MGSNRPKNFR RRGDDGGDEI DGKVATPSSK PTSTLSSSKP KTLSASAPKK KLLSFADDEE
EEEDGAPRVT IKPKNGRDRV KSSSRLGVSG SSHRHSSTKE RRPASSNVLP QAGSYSKEAL
LELQKNTRTL PYSRSSANAE PKVVLKGLIK PPQDHEQQSL KDVVKQVSDL DFDEEGEEEQ
HEDAFADQAA IIRAKKERMR QSRSAPAPDY ISLDGGIVNH SAVEGVSDED ADFQGIFVGP
RPQKDDKKGV FDFGDENPTA KETTTSSIYE DEDEEDKLWE EEQFKKGIGK RMDEGSHRTV
TSNGIGVPLH SKQQTLPQQQ PQMYAYHAGT PMPNVSVAPT IGPATSVDTL PMSQQAELAK
KALKDNVKKL KESHAKTLSS LTKTDENLTA SLMSITALES SLSAAGDKYV FMQKLRDFIS
VICDFMQNKG SLIEEIEDQM KELNEKHALS ILERRIADNN DEMIELGAAV KAAMTVLNKH
GSSSSVIAAA TGAALAASTS IRQQMNQPVK LDEFGRDENL QKRREVEQRA AARQKRRARF
ENKRASAMEV DGPSLKIEGE SSTDESDTET SAYKETRDSL LQCADKVFSD ASEEYSQLSK
VKARFERWKR DYSSTYRDAY MSLTVPSIFS PYVRLELLKW DPLHQDVDFF DMKWHGLLFD
YGKPEDGDDF APDDTDANLV PELVEKVAIP ILHHQIVRCW DILSTRETRN AVAATSLVTN
YVSASSEALA ELFAAIRARL VEAIAAISVP TWDPLVLKAV PNTPQVAAYR FGTSVRLMRN
ICMWKDILAL PVLENLALSD LLFGKVLPHV RSIASNIHDA VTRTERIVAS LSGVWTGPSV
TRTHSRPLQP LVDCTLTLRR ILEKRLGSGL DDAETTGLAR RLKRILVELH EHDHAREIVR
TFNLKEAV
