ID   ILR2_ARATH              Reviewed;         386 AA.
AC   F4J8R4; A0MEW8; Q1PEP6; Q9LS41;
DT   07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=IAA-leucine resistant 2 {ECO:0000303|PubMed:12904214};
DE   AltName: Full=Protein PARTITIVIRUS CP-LIKE SEQUENCE 1 {ECO:0000303|PubMed:21779172};
DE            Short=AtPCLS1 {ECO:0000303|PubMed:21779172};
GN   Name=ILR2 {ECO:0000303|PubMed:12904214};
GN   Synonyms=PCLS1 {ECO:0000303|PubMed:21779172};
GN   OrderedLocusNames=At3g18485 {ECO:0000312|Araport:AT3G18485};
GN   ORFNames=MYF24.21 {ECO:0000312|EMBL:BAB01115.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-224.
RC   STRAIN=cv. Columbia;
RX   PubMed=20810725; DOI=10.1128/jvi.00955-10;
RA   Liu H., Fu Y., Jiang D., Li G., Xie J., Cheng J., Peng Y., Ghabrial S.A.,
RA   Yi X.;
RT   "Widespread horizontal gene transfer from double-stranded RNA viruses to
RT   eukaryotic nuclear genomes.";
RL   J. Virol. 84:11876-11887(2010).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-204.
RC   STRAIN=cv. Columbia;
RX   PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA   Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT   "Simultaneous high-throughput recombinational cloning of open reading
RT   frames in closed and open configurations.";
RL   Plant Biotechnol. J. 4:317-324(2006).
RN   [5]
RP   FUNCTION, MUTAGENESIS OF PRO-319, DISRUPTION PHENOTYPE, AND SUBCELLULAR
RP   LOCATION.
RC   STRAIN=cv. Columbia, and cv. Wassilewskija;
RX   PubMed=12904214; DOI=10.1046/j.1365-313x.2003.01826.x;
RA   Magidin M., Pittman J.K., Hirschi K.D., Bartel B.;
RT   "ILR2, a novel gene regulating IAA conjugate sensitivity and metal
RT   transport in Arabidopsis thaliana.";
RL   Plant J. 35:523-534(2003).
RN   [6]
RP   GENE FAMILY.
RX   PubMed=21779172; DOI=10.1371/journal.ppat.1002146;
RA   Chiba S., Kondo H., Tani A., Saisho D., Sakamoto W., Kanematsu S.,
RA   Suzuki N.;
RT   "Widespread endogenization of genome sequences of non-retroviral RNA
RT   viruses into plant genomes.";
RL   PLoS Pathog. 7:E1002146-E1002146(2011).
CC   -!- FUNCTION: Regulates an ATP-dependent metal transporter (e.g. manganese
CC       transporter). Probably involved in the metabolism of auxin conjugates
CC       such as indole-3-acetic acid-leucine (IAA-Leu) and IAA-Phe. Required
CC       for root development. {ECO:0000269|PubMed:12904214}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:12904214}.
CC   -!- DISRUPTION PHENOTYPE: Recessive resistance to auxin conjugates indole-
CC       3-acetic acid-leucine (IAA-Leu) and IAA-Phe, but normal sensitivity to
CC       free IAA. Defective in lateral root formation and primary root
CC       elongation. Resistant to manganese- and cobalt-mediated inhibition of
CC       root elongation probably due to an enhanced ATP-dependent manganese
CC       transport. {ECO:0000269|PubMed:12904214}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABE65482.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=ABK28217.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=ADI99920.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAB01115.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB026658; BAB01115.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002686; AEE76103.1; -; Genomic_DNA.
DR   EMBL; HM068619; ADI99920.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; DQ446671; ABE65482.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; DQ653091; ABK28217.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; NP_683573.1; NM_148731.1.
DR   AlphaFoldDB; F4J8R4; -.
DR   STRING; 3702.AT3G18485.1; -.
DR   PaxDb; F4J8R4; -.
DR   PRIDE; F4J8R4; -.
DR   EnsemblPlants; AT3G18485.1; AT3G18485.1; AT3G18485.
DR   GeneID; 821378; -.
DR   Gramene; AT3G18485.1; AT3G18485.1; AT3G18485.
DR   KEGG; ath:AT3G18485; -.
DR   Araport; AT3G18485; -.
DR   TAIR; locus:504955735; AT3G18485.
DR   eggNOG; ENOG502SKAP; Eukaryota.
DR   HOGENOM; CLU_716392_0_0_1; -.
DR   InParanoid; F4J8R4; -.
DR   OMA; FWIQCLR; -.
DR   OrthoDB; 1090738at2759; -.
DR   PRO; PR:F4J8R4; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; F4J8R4; baseline and differential.
DR   GO; GO:0005829; C:cytosol; TAS:TAIR.
DR   GO; GO:0010249; P:auxin conjugate metabolic process; IMP:TAIR.
DR   GO; GO:0010102; P:lateral root morphogenesis; IMP:TAIR.
DR   GO; GO:0010038; P:response to metal ion; IMP:TAIR.
DR   GO; GO:0048364; P:root development; IMP:TAIR.
PE   1: Evidence at protein level;
KW   Cytoplasm; Reference proteome.
FT   CHAIN           1..386
FT                   /note="IAA-leucine resistant 2"
FT                   /id="PRO_0000437142"
FT   MUTAGEN         319
FT                   /note="P->L: In ilr2-1; resistance to the auxin conjugates
FT                   indole-3-acetic acid-leucine (IAA-Leu) and increased
FT                   resistance to cobalt."
FT                   /evidence="ECO:0000269|PubMed:12904214"
SQ   SEQUENCE   386 AA;  43389 MW;  F7D82CB661CE1F4F CRC64;
     MASESSTHKT KLEKKTCLSF LSSFTRSQIV YPTQGISHYY PSCHMMVHAM NITLCDNFDF
     KRANPNYHPY ILRLYCGVLF WIQCLRAGND VNDLTDVQHR FLNRFLDNHP LETLAVPGPL
     LGLFKTLCSS QPEFPHNHNG KVYPRIPAQP GPARRDAFMR DLLESHFLPN VPGIFALLED
     LTAFLPNTSR LSKERQAHSR YPKYPDQSLN EAFADCYSNF NFQVTSAADN LEKISSFLHM
     KHSMAWFNQV KGVADDVAAS FEGSGTLADC SPHGLVANQV MVVLSTPEHL PESPNCIADK
     RATYEFGYQL KSTVRNLPPL AEALAAFSQT YIRMFPNHPF FGTFGSKTLD HGPFWKIRPI
     GSSLTDNSSY LTIPSIVKQA FKGPAT