APO1_ORYSI
ID APO1_ORYSI Reviewed; 429 AA.
AC B8B183;
DT 03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=Protein ABERRANT PANICLE ORGANIZATION 1 {ECO:0000250|UniProtKB:Q655Y0};
DE AltName: Full=F-box protein 321 {ECO:0000250|UniProtKB:Q655Y0};
DE Short=OsFbox321 {ECO:0000250|UniProtKB:Q655Y0};
DE AltName: Full=Protein STRONG CULM 2 {ECO:0000250|UniProtKB:Q655Y0};
GN Name=APO1 {ECO:0000250|UniProtKB:Q655Y0};
GN Synonyms=FBOX321 {ECO:0000250|UniProtKB:Q655Y0},
GN SCM2 {ECO:0000250|UniProtKB:Q655Y0};
GN ORFNames=OsI_24028 {ECO:0000312|EMBL:EEC81133.1};
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [2]
RP FUNCTION.
RC STRAIN=cv. Habataki;
RX PubMed=21119645; DOI=10.1038/ncomms1132;
RA Ookawa T., Hobo T., Yano M., Murata K., Ando T., Miura H., Asano K.,
RA Ochiai Y., Ikeda M., Nishitani R., Ebitani T., Ozaki H., Angeles E.R.,
RA Hirasawa T., Matsuoka M.;
RT "New approach for rice improvement using a pleiotropic QTL gene for lodging
RT resistance and yield.";
RL Nat. Commun. 1:132-132(2010).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF SER-15; ARG-295 AND
RP 315-GLY--GLY-317, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Habataki;
RX PubMed=20151298; DOI=10.1007/s00122-009-1218-8;
RA Terao T., Nagata K., Morino K., Hirose T.;
RT "A gene controlling the number of primary rachis branches also controls the
RT vascular bundle formation and hence is responsible to increase the harvest
RT index and grain yield in rice.";
RL Theor. Appl. Genet. 120:875-893(2010).
RN [4]
RP FUNCTION, AND INDUCTION BY OZONE.
RC STRAIN=cv. Habataki;
RX PubMed=25923431; DOI=10.1371/journal.pone.0123308;
RA Tsukahara K., Sawada H., Kohno Y., Matsuura T., Mori I.C., Terao T.,
RA Ioki M., Tamaoki M.;
RT "Ozone-induced rice grain yield loss is triggered via a change in panicle
RT morphology that is controlled by ABERRANT PANICLE ORGANIZATION 1 gene.";
RL PLoS ONE 10:E0123308-E0123308(2015).
CC -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC complexes, which may mediate the ubiquitination and subsequent
CC proteasomal degradation of target proteins (By similarity). Together
CC with FL/APO2, involved in the temporal regulation of meristem identity
CC during both vegetative and reproductive developments in an APO2-
CC dependent manner (By similarity). Promotes spikelet formation by
CC suppressing the precocious conversion of inflorescence meristems to
CC spikelet meristems, probably via a positive regulation of class-C
CC floral homeotic genes, but not of class-B genes, and through the
CC control of cell proliferation in meristems (PubMed:21119645). Mediates
CC culm development and strength/diameter enhancement at internodes
CC (PubMed:21119645). Required for the regulation of the plastochron,
CC floral organ identity, and floral determinacy (By similarity). Controls
CC the number of primary rachis branches (PRBs) (PubMed:20151298). May
CC trigger the formation of vascular bundle systems which, consequently,
CC promote carbohydrate translocation to panicles (PubMed:20151298).
CC Involved in ozone-induced grain yield regulation (PubMed:25923431).
CC {ECO:0000250|UniProtKB:Q655Y0, ECO:0000250|UniProtKB:Q8LEA8,
CC ECO:0000269|PubMed:20151298, ECO:0000269|PubMed:21119645,
CC ECO:0000269|PubMed:25923431}.
CC -!- PATHWAY: Protein modification; protein ubiquitination. {ECO:0000305}.
CC -!- SUBUNIT: Part of a putative SCF (ASK/Cullin/F-box) ubiquitin ligase
CC complex (By similarity). Interacts with FL/APO2 (By similarity).
CC {ECO:0000250|UniProtKB:Q39090, ECO:0000250|UniProtKB:Q655Y0}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, roots, leaves, shoot apical
CC meristem (SAM), developing panicles, and, at lower levels, in
CC developing seeds. {ECO:0000269|PubMed:20151298}.
CC -!- DEVELOPMENTAL STAGE: Present in the developing vascular bundle systems
CC (PubMed:20151298). In the reproductive phase, first observed in the
CC outer several cell layers of the rachis meristem and primary branch
CC meristems (PubMed:20151298). Confined to panicles primodia and young
CC panicles, particularly in the developing rachis branches
CC (PubMed:20151298). {ECO:0000269|PubMed:20151298}.
CC -!- INDUCTION: Repressed by ozone. {ECO:0000269|PubMed:25923431}.
CC -!- DOMAIN: The F-box is necessary for the interaction with ASK proteins.
CC {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Reduced number of primary rachis branches (PRBs)
CC and of the number of grains per panicle, thus leading to reduced grain
CC yield. {ECO:0000269|PubMed:20151298}.
CC -!- MISCELLANEOUS: Ozone has various impact on different cultivars;
CC O.sativa subsp. japonica cv. Sasanishiki exhibits ozone-induced leaf
CC injury, but no grain yield loss, and, by contrast, O.sativa subsp.
CC indica cv. Habataki has grain yield loss with minimal leaf injury upon
CC ozone treatment. {ECO:0000269|PubMed:25923431}.
CC -!- MISCELLANEOUS: Plants harboring the PBN6 quantitative trait locus (QTL)
CC (e.g. HI1 allele in O.sativa subsp. indica cv. Habataki) exhibit bigger
CC peduncle diameter due to larger vascular bundles and an increased
CC number of primary rachis branches (PRBs) and of the number of grains
CC per panicle, thus leading to increased grain yield.
CC {ECO:0000269|PubMed:20151298}.
CC -!- MISCELLANEOUS: Plants harboring the SCM2 quantitative trait locus (QTL)
CC exhibit both an enhancement of culm strength at internodes and an
CC increased spikelet number leading to higher crop productivity and
CC better grain yield. {ECO:0000269|PubMed:21119645}.
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DR EMBL; CM000131; EEC81133.1; -; Genomic_DNA.
DR AlphaFoldDB; B8B183; -.
DR STRING; 39946.B8B183; -.
DR EnsemblPlants; BGIOSGA020722-TA; BGIOSGA020722-PA; BGIOSGA020722.
DR Gramene; BGIOSGA020722-TA; BGIOSGA020722-PA; BGIOSGA020722.
DR HOGENOM; CLU_038778_2_1_1; -.
DR OMA; ESFHIDA; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000007015; Chromosome 6.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IMP:UniProtKB.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0010074; P:maintenance of meristem identity; IMP:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0042127; P:regulation of cell population proliferation; IEA:EnsemblPlants.
DR GO; GO:0009909; P:regulation of flower development; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:EnsemblPlants.
DR GO; GO:1901342; P:regulation of vasculature development; IMP:UniProtKB.
DR GO; GO:0010193; P:response to ozone; IDA:UniProtKB.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR011043; Gal_Oxase/kelch_b-propeller.
DR SMART; SM00256; FBOX; 1.
DR SUPFAM; SSF50965; SSF50965; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Differentiation; Flowering; Kelch repeat;
KW Membrane; Reference proteome; Repeat; Transmembrane; Transmembrane helix;
KW Ubl conjugation pathway.
FT CHAIN 1..429
FT /note="Protein ABERRANT PANICLE ORGANIZATION 1"
FT /id="PRO_0000447617"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 25..71
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REPEAT 229..277
FT /note="Kelch 1"
FT /evidence="ECO:0000255"
FT REPEAT 284..339
FT /note="Kelch 2"
FT /evidence="ECO:0000255"
FT REPEAT 350..397
FT /note="Kelch 3"
FT /evidence="ECO:0000255"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 15
FT /note="S->P: In PBN6; bigger peduncle diameter due to
FT larger vascular bundles, increased number of primary rachis
FT branches (PRBs) and of the number of grains per panicle,
FT thus leading to increased grain yield; when associated with
FT C-295 and 315-G--G-317 DEL."
FT /evidence="ECO:0000269|PubMed:20151298"
FT MUTAGEN 295
FT /note="R->C: In PBN6; bigger peduncle diameter due to
FT larger vascular bundles, increased number of primary rachis
FT branches (PRBs) and of the number of grains per panicle,
FT thus leading to increased grain yield; when associated with
FT P-15 and 315-G--G-317 DEL."
FT /evidence="ECO:0000269|PubMed:20151298"
FT MUTAGEN 315..317
FT /note="Missing: In PBN6; bigger peduncle diameter due to
FT larger vascular bundles, increased number of primary rachis
FT branches (PRBs) and of the number of grains per panicle,
FT thus leading to increased grain yield; when associated with
FT P-15 and C-295."
FT /evidence="ECO:0000269|PubMed:20151298"
SQ SEQUENCE 429 AA; 44909 MW; 63B68097727E027B CRC64;
MMNPRRLPPL PSSTSSASAA DDMDPRVWRR LPQPLVDRVL ACLPTPSFLR LRAACRRFYH
LLFSSPFLHS HLLLSPHLPF FAFVVPAAGH LLLLDPTATA SWSRLPLPLP PVAGGPAAFS
PAAASAGLLA FLSDASGHKT LLLANPITRL LAALPISPTP RLSPTVGLAA GPTSIIAVVA
GDDLVSPFAV KNISADTFVA DAASVPPSGF WAPSSLLPRL SSLDPGAGMA FASGRFYCMS
SSPFAVLVFD VAENVWSKVQ PPMRRFLRSP ALVELGGGRE GAARVALVSA VEKSRLSVPR
SVRLWTLRGG GGGGGGGAWT EVARMPPEVH AQFAAAEGGR GFECAAHGDY VVLAPRGPVA
QAPTSALVFD SRRDEWRWAP PCPYVVVAHH GGAGAAGFRV FAYEPRLATP AIGLLDATAP
VALHGMHDG
