ID   APO2_ARATH              Reviewed;         440 AA.
AC   Q8W4A5; Q94JR8; Q9FJM2;
DT   05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=APO protein 2, chloroplastic;
DE   AltName: Full=Accumulation of photosystem I protein 2;
DE   AltName: Full=Protein EMBRYO DEFECTIVE 1629;
DE   Flags: Precursor;
GN   Name=APO2; Synonyms=EMB1629; OrderedLocusNames=At5g57930;
GN   ORFNames=MTI20.19;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA   Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT   features of the regions of 1,367,185 bp covered by 19 physically assigned
RT   P1 and TAC clones.";
RL   DNA Res. 5:203-216(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP   OF 1-250 (ISOFORM 2).
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
CC   -!- FUNCTION: May be involved in the stable assembly of several 4Fe-4S
CC       cluster-containing complexes of chloroplasts. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q8W4A5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8W4A5-2; Sequence=VSP_014479;
CC   -!- DOMAIN: The APO repeats may provide ligands for 4Fe-4S centers.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the APO family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00832}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB08863.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB013396; BAB08863.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED96972.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED96973.1; -; Genomic_DNA.
DR   EMBL; AF375421; AAK53005.1; -; mRNA.
DR   EMBL; AY062701; AAL32779.1; -; mRNA.
DR   EMBL; AY114672; AAM47991.1; -; mRNA.
DR   RefSeq; NP_200601.3; NM_125178.6. [Q8W4A5-2]
DR   RefSeq; NP_851208.1; NM_180877.2. [Q8W4A5-1]
DR   AlphaFoldDB; Q8W4A5; -.
DR   SMR; Q8W4A5; -.
DR   STRING; 3702.AT5G57930.2; -.
DR   PaxDb; Q8W4A5; -.
DR   PRIDE; Q8W4A5; -.
DR   ProteomicsDB; 246778; -. [Q8W4A5-1]
DR   EnsemblPlants; AT5G57930.1; AT5G57930.1; AT5G57930. [Q8W4A5-1]
DR   EnsemblPlants; AT5G57930.2; AT5G57930.2; AT5G57930. [Q8W4A5-2]
DR   GeneID; 835904; -.
DR   Gramene; AT5G57930.1; AT5G57930.1; AT5G57930. [Q8W4A5-1]
DR   Gramene; AT5G57930.2; AT5G57930.2; AT5G57930. [Q8W4A5-2]
DR   KEGG; ath:AT5G57930; -.
DR   Araport; AT5G57930; -.
DR   TAIR; locus:2174438; AT5G57930.
DR   eggNOG; ENOG502QPNK; Eukaryota.
DR   HOGENOM; CLU_033199_0_1_1; -.
DR   InParanoid; Q8W4A5; -.
DR   OMA; GHPFRSC; -.
DR   PhylomeDB; Q8W4A5; -.
DR   PRO; PR:Q8W4A5; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q8W4A5; baseline and differential.
DR   Genevisible; Q8W4A5; AT.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   InterPro; IPR023342; APO_dom.
DR   Pfam; PF05634; APO_RNA-bind; 2.
DR   PROSITE; PS51499; APO; 2.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Chloroplast; Plastid; Reference proteome; Repeat;
KW   Transit peptide.
FT   TRANSIT         1..62
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           63..440
FT                   /note="APO protein 2, chloroplastic"
FT                   /id="PRO_0000001931"
FT   DOMAIN          162..247
FT                   /note="APO 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00832"
FT   DOMAIN          332..417
FT                   /note="APO 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00832"
FT   REGION          106..126
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        106..121
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         9..10
FT                   /note="SF -> SSSTV (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_014479"
SQ   SEQUENCE   440 AA;  49938 MW;  AF686442C417D840 CRC64;
     MSITYSAISF SGFSPKSVPF AIHSVTRRQF LNPNTFYRFG FSPSLQGSSI EFSLQLNSRV
     VLSKERRSLP LVVRNDRPQN EDLPKQYTRR EKKPFPVPIV DLRRAARERV KNNKDKPKRP
     LPPPKNGMVV KSLVPLAYKV YNARIRLINN LHRLMKVVRV NACGWCNEIH VGPYGHPFKS
     CKGPNTSQRK GLHEWTNSVI EDVIVPLEAY HLFDRLGKRI RHDERFSIPR VPAVVELCIQ
     GGVEIPEFPA KRRRKPIIRI GKSEFVDADE TELPDPEPQP PPVPLLTELP VSEITPPSSE
     EETVSLAEET LQAWEEMRAG AKKLMRMYRV RVCGYCPEVH VGPTGHKAQN CGAFKHQQRN
     GQHGWQSAVL DDLIPPRYVW HVPDVNGPPM QRELRSFYGQ APAVVEICAQ AGAVVPEHYR
     ATMRLEVGIP SSVKEAEMVV