4EBP3_HUMAN
ID 4EBP3_HUMAN Reviewed; 100 AA.
AC O60516;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Eukaryotic translation initiation factor 4E-binding protein 3;
DE Short=4E-BP3;
DE Short=eIF4E-binding protein 3;
GN Name=EIF4EBP3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH EIF4E, MUTAGENESIS OF TYR-40
RP AND LEU-45, TISSUE SPECIFICITY, AND PHOSPHORYLATION.
RX PubMed=9593750; DOI=10.1074/jbc.273.22.14002;
RA Poulin F., Gingras A.-C., Olsen H., Chevalier S., Sonenberg N.;
RT "4E-BP3, a new member of the eukaryotic initiation factor 4E-binding
RT protein family.";
RL J. Biol. Chem. 273:14002-14007(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Repressor of translation initiation that regulates EIF4E
CC activity by preventing its assembly into the eIF4F complex:
CC hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds
CC to EIF4E, leading to repress translation. In contrast,
CC hyperphosphorylated form dissociates from EIF4E, allowing interaction
CC between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation.
CC {ECO:0000250|UniProtKB:Q13541}.
CC -!- SUBUNIT: EIF4EBP3 interacts with EIF4E. {ECO:0000269|PubMed:9593750}.
CC -!- INTERACTION:
CC O60516; P06730: EIF4E; NbExp=9; IntAct=EBI-746950, EBI-73440;
CC O60516; P15927: RPA2; NbExp=3; IntAct=EBI-746950, EBI-621404;
CC O60516; Q80ZJ3: Eif4e2; Xeno; NbExp=2; IntAct=EBI-746950, EBI-934970;
CC -!- TISSUE SPECIFICITY: Expression is highest in skeletal muscle, heart,
CC kidney, and pancreas, whereas there is very little expression in brain
CC and thymus. {ECO:0000269|PubMed:9593750}.
CC -!- PTM: Phosphorylated. {ECO:0000269|PubMed:9593750}.
CC -!- SIMILARITY: Belongs to the eIF4E-binding protein family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF038869; AAC39761.1; -; mRNA.
DR EMBL; BC010881; AAH10881.1; -; mRNA.
DR EMBL; BC069293; AAH69293.1; -; mRNA.
DR EMBL; BC073751; AAH73751.1; -; mRNA.
DR CCDS; CCDS4226.1; -.
DR RefSeq; NP_003723.1; NM_003732.2.
DR AlphaFoldDB; O60516; -.
DR SMR; O60516; -.
DR BioGRID; 114190; 10.
DR ELM; O60516; -.
DR IntAct; O60516; 5.
DR MINT; O60516; -.
DR STRING; 9606.ENSP00000308472; -.
DR iPTMnet; O60516; -.
DR PhosphoSitePlus; O60516; -.
DR BioMuta; EIF4EBP3; -.
DR EPD; O60516; -.
DR jPOST; O60516; -.
DR MassIVE; O60516; -.
DR PaxDb; O60516; -.
DR PeptideAtlas; O60516; -.
DR PRIDE; O60516; -.
DR ProteomicsDB; 49454; -.
DR Antibodypedia; 34915; 131 antibodies from 18 providers.
DR DNASU; 8637; -.
DR Ensembl; ENST00000310331.3; ENSP00000308472.2; ENSG00000243056.2.
DR GeneID; 8637; -.
DR KEGG; hsa:8637; -.
DR MANE-Select; ENST00000310331.3; ENSP00000308472.2; NM_003732.3; NP_003723.1.
DR UCSC; uc003lfy.1; human.
DR CTD; 8637; -.
DR DisGeNET; 8637; -.
DR GeneCards; EIF4EBP3; -.
DR HGNC; HGNC:3290; EIF4EBP3.
DR HPA; ENSG00000243056; Low tissue specificity.
DR MIM; 603483; gene.
DR neXtProt; NX_O60516; -.
DR OpenTargets; ENSG00000243056; -.
DR PharmGKB; PA27718; -.
DR VEuPathDB; HostDB:ENSG00000243056; -.
DR eggNOG; ENOG502S03G; Eukaryota.
DR GeneTree; ENSGT00940000163328; -.
DR HOGENOM; CLU_111706_0_0_1; -.
DR InParanoid; O60516; -.
DR OMA; YSTTPHG; -.
DR OrthoDB; 1597535at2759; -.
DR PhylomeDB; O60516; -.
DR TreeFam; TF101530; -.
DR PathwayCommons; O60516; -.
DR SignaLink; O60516; -.
DR SIGNOR; O60516; -.
DR BioGRID-ORCS; 8637; 6 hits in 1075 CRISPR screens.
DR GeneWiki; EIF4EBP3; -.
DR GenomeRNAi; 8637; -.
DR Pharos; O60516; Tbio.
DR PRO; PR:O60516; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; O60516; protein.
DR Bgee; ENSG00000243056; Expressed in right uterine tube and 92 other tissues.
DR Genevisible; O60516; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016281; C:eukaryotic translation initiation factor 4F complex; NAS:UniProtKB.
DR GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR GO; GO:0008190; F:eukaryotic initiation factor 4E binding; IBA:GO_Central.
DR GO; GO:0030371; F:translation repressor activity; NAS:UniProtKB.
DR GO; GO:0045947; P:negative regulation of translational initiation; IBA:GO_Central.
DR InterPro; IPR008606; EIF4EBP.
DR Pfam; PF05456; eIF_4EBP; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Protein synthesis inhibitor; Reference proteome;
KW Translation regulation.
FT CHAIN 1..100
FT /note="Eukaryotic translation initiation factor 4E-binding
FT protein 3"
FT /id="PRO_0000190518"
FT REGION 81..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 40..46
FT /note="YXXXXLphi motif"
FT /evidence="ECO:0000269|PubMed:9593750"
FT MOTIF 96..100
FT /note="TOS motif"
FT /evidence="ECO:0000250|UniProtKB:Q13541"
FT COMPBIAS 85..100
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 40
FT /note="Y->A: Loss of interaction with EIF4E."
FT /evidence="ECO:0000269|PubMed:9593750"
FT MUTAGEN 45
FT /note="L->A: Loss of interaction with EIF4E."
FT /evidence="ECO:0000269|PubMed:9593750"
SQ SEQUENCE 100 AA; 10873 MW; FD29A7F3829E546E CRC64;
MSTSTSCPIP GGRDQLPDCY STTPGGTLYA TTPGGTRIIY DRKFLLECKN SPIARTPPCC
LPQIPGVTTP PTAPLSKLEE LKEQETEEEI PDDAQFEMDI
