ID   ILVD1_RHIME             Reviewed;         574 AA.
AC   Q92RP0;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Putative dehydratase IlvD1;
DE            EC=4.2.1.-;
GN   Name=ilvD1; OrderedLocusNames=R00822; ORFNames=SMc00884;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481430; DOI=10.1073/pnas.161294398;
RA   Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA   Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA   Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA   Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA   Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT   "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT   meliloti strain 1021.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=1021;
RX   PubMed=22797764; DOI=10.1128/jb.00982-12;
RA   Geddes B.A., Oresnik I.J.;
RT   "Inability to catabolize galactose leads to increased ability to compete
RT   for nodule occupancy in Sinorhizobium meliloti.";
RL   J. Bacteriol. 194:5044-5053(2012).
CC   -!- FUNCTION: Involved in the degradation of galactose via the DeLey-
CC       Doudoroff pathway. {ECO:0000305}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene exhibit no detectable
CC       phenotype when grown on galactose as sole carbon source.
CC       {ECO:0000269|PubMed:22797764}.
CC   -!- SIMILARITY: Belongs to the IlvD/Edd family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL591688; CAC45394.1; -; Genomic_DNA.
DR   RefSeq; NP_384928.1; NC_003047.1.
DR   RefSeq; WP_010968847.1; NC_003047.1.
DR   AlphaFoldDB; Q92RP0; -.
DR   SMR; Q92RP0; -.
DR   STRING; 266834.SMc00884; -.
DR   EnsemblBacteria; CAC45394; CAC45394; SMc00884.
DR   GeneID; 61602288; -.
DR   KEGG; sme:SMc00884; -.
DR   PATRIC; fig|266834.11.peg.2212; -.
DR   eggNOG; COG0129; Bacteria.
DR   HOGENOM; CLU_014271_3_1_5; -.
DR   OMA; MEHFHHA; -.
DR   Proteomes; UP000001976; Chromosome.
DR   GO; GO:0016836; F:hydro-lyase activity; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019752; P:carboxylic acid metabolic process; IEA:UniProt.
DR   GO; GO:0044238; P:primary metabolic process; IEA:UniProt.
DR   Gene3D; 3.50.30.80; -; 1.
DR   InterPro; IPR042096; Dihydro-acid_dehy_C.
DR   InterPro; IPR000581; DiOHA_6PGluconate_deHydtase.
DR   InterPro; IPR020558; DiOHA_6PGluconate_deHydtase_CS.
DR   InterPro; IPR037237; IlvD/EDD_N.
DR   Pfam; PF00920; ILVD_EDD; 1.
DR   SUPFAM; SSF143975; SSF143975; 1.
DR   PROSITE; PS00886; ILVD_EDD_1; 1.
PE   3: Inferred from homology;
KW   Iron; Iron-sulfur; Lyase; Metal-binding; Reference proteome.
FT   CHAIN           1..574
FT                   /note="Putative dehydratase IlvD1"
FT                   /id="PRO_0000428933"
FT   BINDING         124
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255"
FT   BINDING         197
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   574 AA;  62034 MW;  AACA1D2123C9CED7 CRC64;
     MSDKKKELRS RHWYGGTHKD GFIHRSWMKN QGFPDHVFDG RPIIGICNTW SELTPCNSHL
     RILAEGVKRG VWEAGGFPVE FPVSSLGETQ MRPTAMLFRN LLAMDVEEAI RAYGIDGVVL
     LGGCDKTTPG QLMGAASVDL PTIVVSSGPM LNGKWKGKDI GSGTDVWKFS EAVRAGEMSL
     QEFMAAESGM SRSPGVCMTM GTATTMASIV EAMGLSLPTN AALPAVDARR MALSHMTGKR
     IVEMVHEDLR LSKILTKENF ENGIIANAAV GGSTNAVVHM LAIAGRAGID LCLEDFDRVG
     GQVPCIVNCM PSGKYLIEDL AYAGGLPAVM NRIQHLLHPD APTVFGVPIS KYWEAAEVYN
     DDVIRPLDNP LRAAAGIRVL KGNLAPNGAV IKPSAASEHL LAHEGPAYVF DTIEDLRAKI
     DDPDLPVTED TILVLKGCGP KGYPGMAEVG NMPIPRRLVE KGVRDMVRIS DARMSGTAFG
     TVVLHVSPEA NAGGPLAIVR TGDRIRLDAL KGELNLLVSE EELAARMAAW QPPEQKWHRG
     YYKLYHDTVL QADKGADLDF LVGKSGSEVL RESH