ID   APOA4_NEOSC             Reviewed;         382 AA.
AC   A0A2Y9HKB5;
DT   11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT   12-SEP-2018, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Apolipoprotein A-IV;
DE            Short=Apo-AIV;
DE            Short=ApoA-IV;
DE   AltName: Full=Apolipoprotein A4;
DE   Flags: Precursor;
GN   Name=APOA4;
OS   Neomonachus schauinslandi (Hawaiian monk seal) (Monachus schauinslandi).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Carnivora; Caniformia; Phocidae; Neomonachus.
OX   NCBI_TaxID=29088;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   TISSUE=Blood;
RA   Mohr D.W., Scott A.F.;
RT   "Improved de novo genome assembly: linked-read sequencing combined with
RT   optical mapping produce a high quality mammalian genome at relatively low
RT   cost.";
RL   Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   IDENTIFICATION.
RA   Puppione D.L.;
RL   Unpublished observations (OCT-2019).
CC   -!- FUNCTION: May have a role in chylomicrons and VLDL secretion and
CC       catabolism. Required for efficient activation of lipoprotein lipase by
CC       ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL
CC       and chylomicrons. {ECO:0000250|UniProtKB:P33621}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P06727}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P33621}.
CC   -!- DOMAIN: Nine of the thirteen 22-amino acid tandem repeats (each 22-mer
CC       is actually a tandem array of two, A and B, related 11-mers) occurring
CC       in this sequence are predicted to be highly alpha-helical, and many of
CC       these helices are amphipathic. They may therefore serve as lipid-
CC       binding domains with lecithin:cholesterol acyltransferase (LCAT)
CC       activating abilities. {ECO:0000250|UniProtKB:P33621}.
CC   -!- PTM: Phosphorylation sites are present in the extracellular medium.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the apolipoprotein A1/A4/E family.
CC       {ECO:0000305}.
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DR   EMBL; NINY01000000; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; A0A2Y9HKB5; -.
DR   SMR; A0A2Y9HKB5; -.
DR   Proteomes; UP000248481; Genome assembly.
DR   GO; GO:0042627; C:chylomicron; IEA:UniProtKB-KW.
DR   GO; GO:0034364; C:high-density lipoprotein particle; IEA:UniProtKB-KW.
DR   GO; GO:0008289; F:lipid binding; IEA:InterPro.
DR   GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR   GO; GO:0042157; P:lipoprotein metabolic process; IEA:InterPro.
DR   InterPro; IPR000074; ApoA_E.
DR   Pfam; PF01442; Apolipoprotein; 2.
PE   3: Inferred from homology;
KW   Chylomicron; HDL; Lipid transport; Phosphoprotein; Reference proteome;
KW   Repeat; Secreted; Signal; Transport.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..382
FT                   /note="Apolipoprotein A-IV"
FT                   /id="PRO_5016013014"
FT   REPEAT          33..54
FT                   /note="1"
FT   REPEAT          60..81
FT                   /note="2"
FT   REPEAT          82..103
FT                   /note="3"
FT   REPEAT          115..136
FT                   /note="4"
FT   REPEAT          137..158
FT                   /note="5"
FT   REPEAT          159..180
FT                   /note="6"
FT   REPEAT          181..202
FT                   /note="7"
FT   REPEAT          203..224
FT                   /note="8"
FT   REPEAT          225..246
FT                   /note="9"
FT   REPEAT          247..268
FT                   /note="10"
FT   REPEAT          269..286
FT                   /note="11"
FT   REPEAT          287..308
FT                   /note="12"
FT   REPEAT          309..330
FT                   /note="13"
FT   REGION          33..330
FT                   /note="13 X 22 AA approximate tandem repeats"
SQ   SEQUENCE   382 AA;  43657 MW;  126E43EFFD5D7750 CRC64;
     MFLKAVVLTL SLVAVTGAQA EVSANQVATV VWDYFSQLSN NAKEAVEHLQ KSELTQQLNA
     LFQDKIGQVN TYTDNLQKKL VPFATELHER LRKDSEKLKE EIRKELEELR ARLLPHADEV
     SRKIGDNMHE LQQRLGPYAE ELRTQVNTHA ERLRNQLTAH AQSLETTLRQ NVDNLQASLT
     PYADELKAKI DQNVEELKGH LTPYADELKV KIDQNVEDLR RSLAPYAQDV QEKLNHQLEG
     LAFQMKKNAE ELKAKISANA DELRQKLVPV AEVVRGKLRD NTEELQKSLA ELSSHLDRQV
     EEFRRNVGPY GETFNKALLQ QVEELRQKLG PYAGDVEDHL SFLEKDLRDK VNSFFSTLEE
     KENQDMLVAV PELQLTPVPL ES