ID   APOA4_PHOVI             Reviewed;         382 AA.
AC   P0DTS1;
DT   11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT   11-DEC-2019, sequence version 1.
DT   25-MAY-2022, entry version 5.
DE   RecName: Full=Apolipoprotein A-IV;
DE            Short=Apo-AIV;
DE            Short=ApoA-IV;
DE   AltName: Full=Apolipoprotein A4;
DE   Flags: Precursor;
GN   Name=APOA4;
OS   Phoca vitulina (Harbor seal).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Carnivora; Caniformia; Phocidae; Phoca.
OX   NCBI_TaxID=9720;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   TISSUE=Blood;
RA   Culibrk L., Leelakumari S., Taylor G.A., Tse K., Cheng D., Chuah E.,
RA   Kirk H., Pandoh P., Troussard A., Zhao Y., Mungall A., Moore R.,
RA   Akhurst L., Marra M.A., Haulena M., Jones S.J.M.;
RT   "The genome of the Harbour Seal (Phoca vitulina).";
RL   Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   IDENTIFICATION.
RA   Puppione D.L.;
RL   Unpublished observations (OCT-2019).
CC   -!- FUNCTION: May have a role in chylomicrons and VLDL secretion and
CC       catabolism. Required for efficient activation of lipoprotein lipase by
CC       ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL
CC       and chylomicrons. {ECO:0000250|UniProtKB:P33621}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P06727}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P33621}.
CC   -!- DOMAIN: Nine of the thirteen 22-amino acid tandem repeats (each 22-mer
CC       is actually a tandem array of two, A and B, related 11-mers) occurring
CC       in this sequence are predicted to be highly alpha-helical, and many of
CC       these helices are amphipathic. They may therefore serve as lipid-
CC       binding domains with lecithin:cholesterol acyltransferase (LCAT)
CC       activating abilities. {ECO:0000250|UniProtKB:P33621}.
CC   -!- PTM: Phosphorylation sites are present in the extracellular medium.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the apolipoprotein A1/A4/E family.
CC       {ECO:0000305}.
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DR   EMBL; ML169427; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P0DTS1; -.
DR   SMR; P0DTS1; -.
DR   GO; GO:0042627; C:chylomicron; IEA:UniProtKB-KW.
DR   GO; GO:0034364; C:high-density lipoprotein particle; IEA:UniProtKB-KW.
DR   GO; GO:0008289; F:lipid binding; IEA:InterPro.
DR   GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR   GO; GO:0042157; P:lipoprotein metabolic process; IEA:InterPro.
DR   InterPro; IPR000074; ApoA_E.
DR   Pfam; PF01442; Apolipoprotein; 2.
PE   3: Inferred from homology;
KW   Chylomicron; HDL; Lipid transport; Phosphoprotein; Repeat; Secreted;
KW   Signal; Transport.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..382
FT                   /note="Apolipoprotein A-IV"
FT                   /id="PRO_0000448768"
FT   REPEAT          33..54
FT                   /note="1"
FT   REPEAT          60..81
FT                   /note="2"
FT   REPEAT          82..103
FT                   /note="3"
FT   REPEAT          115..136
FT                   /note="4"
FT   REPEAT          137..158
FT                   /note="5"
FT   REPEAT          159..180
FT                   /note="6"
FT   REPEAT          181..202
FT                   /note="7"
FT   REPEAT          203..224
FT                   /note="8"
FT   REPEAT          225..246
FT                   /note="9"
FT   REPEAT          247..268
FT                   /note="10"
FT   REPEAT          269..286
FT                   /note="11"
FT   REPEAT          287..308
FT                   /note="12"
FT   REPEAT          309..330
FT                   /note="13"
FT   REGION          33..330
FT                   /note="13 X 22 AA approximate tandem repeats"
SQ   SEQUENCE   382 AA;  43620 MW;  24D07EDF080CA306 CRC64;
     MFLKAVVLTL SLVAVTGAQA EVSANQVATV VWDYFSQLSN NAKEAVEHLQ KSELTQQLNA
     LFQDKIGQVN TYTDNLQKKL VSFAMELHER LRKDSEKLKE EIRKELEELR AGLLPHADEV
     SRKIGDNMHE LQQRLGPYAE ELRTQVNTHA EHLRNQLTAH AQRMETTLRQ NVGNLQASLT
     PYADELKAKI DQNVEELKGH LTPYADELKV KIDQNVEDLR RSLAPYAQDV QEKLNHQLEG
     LAFQMKKNAE ELKAKISANA DELRQKLVPV AEVVRGKLRD NTEELQKSLA ELSSHLDRQV
     EEFRRNMGPY GETFNKALLQ QVEELRQKLG PYAGDVEDHL SFLEKDLRDK VNSFFSTLEE
     KENQDMLVAV PELQLTPVPL ES