ILVH_SALTY
ID ILVH_SALTY Reviewed; 163 AA.
AC P21622; Q9JP76;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Acetolactate synthase isozyme 3 small subunit;
DE EC=2.2.1.6;
DE AltName: Full=ALS-III;
DE AltName: Full=Acetohydroxy-acid synthase III small subunit;
DE Short=AHAS-III;
GN Name=ilvH; OrderedLocusNames=STM0117;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX PubMed=1851954; DOI=10.1007/bf00273623;
RA Jahreis K., Postma P.W., Lengeler J.W.;
RT "Nucleotide sequence of the ilvH-fruR gene region of Escherichia coli K12
RT and Salmonella typhimurium LT2.";
RL Mol. Gen. Genet. 226:332-336(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RA El Hanafi D., Maloriol D., Figueroa-Bossi N., Bossi L.;
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2;
CC Xref=Rhea:RHEA:25249, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:58476; EC=2.2.1.6;
CC -!- ACTIVITY REGULATION: Sensitive to valine inhibition.
CC -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-
CC isoleucine from 2-oxobutanoate: step 1/4.
CC -!- PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine from
CC pyruvate: step 1/4.
CC -!- SUBUNIT: Dimer of large and small chains.
CC -!- MISCELLANEOUS: S.typhimurium contains genes for 3 AHAS isozymes: ilvBN,
CC ilvGM and ilvIH.
CC -!- SIMILARITY: Belongs to the acetolactate synthase small subunit family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF65177.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X55456; CAA39102.1; -; Genomic_DNA.
DR EMBL; AF117227; AAF65177.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE006468; AAL19081.1; -; Genomic_DNA.
DR PIR; S15940; S15940.
DR RefSeq; NP_459122.1; NC_003197.2.
DR RefSeq; WP_001563086.1; NC_003197.2.
DR AlphaFoldDB; P21622; -.
DR SMR; P21622; -.
DR STRING; 99287.STM0117; -.
DR PaxDb; P21622; -.
DR EnsemblBacteria; AAL19081; AAL19081; STM0117.
DR GeneID; 1251635; -.
DR KEGG; stm:STM0117; -.
DR PATRIC; fig|99287.12.peg.122; -.
DR HOGENOM; CLU_055003_1_3_6; -.
DR OMA; EPYGIKE; -.
DR PhylomeDB; P21622; -.
DR BioCyc; SENT99287:STM0117-MON; -.
DR UniPathway; UPA00047; UER00055.
DR UniPathway; UPA00049; UER00059.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003984; F:acetolactate synthase activity; IBA:GO_Central.
DR GO; GO:1990610; F:acetolactate synthase regulator activity; IEA:InterPro.
DR GO; GO:0009097; P:isoleucine biosynthetic process; IBA:GO_Central.
DR GO; GO:0009099; P:valine biosynthetic process; IBA:GO_Central.
DR CDD; cd04878; ACT_AHAS; 1.
DR Gene3D; 3.30.70.1150; -; 1.
DR InterPro; IPR004789; Acetalactate_synth_ssu.
DR InterPro; IPR027271; Acetolactate_synth/TF_NikR_C.
DR InterPro; IPR019455; Acetolactate_synth_ssu_C.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR002912; ACT_dom.
DR InterPro; IPR039557; AHAS_ACT.
DR PANTHER; PTHR30239; PTHR30239; 1.
DR Pfam; PF01842; ACT; 1.
DR Pfam; PF10369; ALS_ss_C; 1.
DR SUPFAM; SSF55021; SSF55021; 2.
DR TIGRFAMs; TIGR00119; acolac_sm; 1.
DR PROSITE; PS51671; ACT; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW Reference proteome; Transferase.
FT CHAIN 1..163
FT /note="Acetolactate synthase isozyme 3 small subunit"
FT /id="PRO_0000151411"
FT DOMAIN 4..78
FT /note="ACT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01007"
FT CONFLICT 91..92
FT /note="IQ -> ME (in Ref. 1; CAA39102)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 163 AA; 18063 MW; 82066840D433D6B5 CRC64;
MRRILSVLLE NESGALSRVI GLFSQRGYNI ESLTVAPTDD PTLSRMTIQT VGDEKVLEQI
EKQLHKLVDV LRVSELGQGA HVEREIMLVK IQASGYGREE VKRNTEIFRG QIIDVTPTLY
TVQLAGTSDK LDAFLASLRD VAKIVEVARS GVVGLSRGDK IMR
