IMA1A_ORYSJ
ID IMA1A_ORYSJ Reviewed; 526 AA.
AC Q71VM4; O82783;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 2.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Importin subunit alpha-1a;
GN OrderedLocusNames=Os01g0253300, LOC_Os01g14950;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, AND
RP INDUCTION.
RC STRAIN=cv. Nipponbare; TISSUE=Callus, and Leaf;
RX PubMed=9678973; DOI=10.1016/s0378-1119(98)00175-9;
RA Shoji K., Iwasaki T., Matsuki R., Miyao M., Yamamoto N.;
RT "Cloning of a cDNA encoding an importin-alpha and down-regulation of the
RT gene by light in rice leaves.";
RL Gene 212:279-286(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Fischer R.;
RT "Importin alpha-3, a novel variant of the small importin subunit
RT evolutionarily conserved from hydrozoa to man.";
RL Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=9727027; DOI=10.1074/jbc.273.37.24083;
RA Jiang C.-J., Imamoto N., Matsuki R., Yoneda Y., Yamamoto N.;
RT "Functional characterization of a plant importin alpha homologue. Nuclear
RT localization signal (NLS)-selective binding and mediation of nuclear import
RT of nls proteins in vitro.";
RL J. Biol. Chem. 273:24083-24087(1998).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND INTERACTION WITH IMPORTIN
RP BETA-1.
RX PubMed=11124253; DOI=10.1074/jbc.m006430200;
RA Jiang C.-J., Shoji K., Matsuki R., Baba A., Inagaki N., Ban H., Iwasaki T.,
RA Imamoto N., Yoneda Y., Deng X.-W., Yamamoto N.;
RT "Molecular cloning of a novel importin alpha homologue from rice, by which
RT constitutive photomorphogenic 1 (COP1) nuclear localization signal (NLS)-
RT protein is preferentially nuclear imported.";
RL J. Biol. Chem. 276:9322-9329(2001).
RN [8]
RP INTERACTION WITH MUNGBEAN YELLOW MOSAIC VIRUS CAPSID PROTEIN.
RX PubMed=15914861; DOI=10.1099/vir.0.80920-0;
RA Guerra-Peraza O., Kirk D., Seltzer V., Veluthambi K., Schmit A.C., Hohn T.,
RA Herzog E.;
RT "Coat proteins of Rice tungro bacilliform virus and Mungbean yellow mosaic
RT virus contain multiple nuclear-localization signals and interact with
RT importin alpha.";
RL J. Gen. Virol. 86:1815-1826(2005).
CC -!- FUNCTION: Functions in nuclear protein import. Binds specifically and
CC directly to substrates containing either a simple or bipartite NLS
CC motif. Promotes docking of import substrates to the nuclear envelope.
CC {ECO:0000269|PubMed:11124253, ECO:0000269|PubMed:9727027}.
CC -!- SUBUNIT: Forms a complex with importin subunit beta-1. The whole
CC complex, most stable and composed of importin alpha, importin beta and
CC NLS substrate, is referred to as PTAC or pore targeting complex.
CC Interacts with mungbean yellow mosaic virus capsid protein.
CC {ECO:0000269|PubMed:11124253, ECO:0000269|PubMed:15914861}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region
CC {ECO:0000269|PubMed:9727027}.
CC -!- TISSUE SPECIFICITY: Highly expressed in callus, followed by root and
CC etiolated leaf. Low expression in green leaf.
CC {ECO:0000269|PubMed:11124253, ECO:0000269|PubMed:9678973}.
CC -!- INDUCTION: In both etiolated and green leaves, down-regulated by light.
CC In green leaf, increased by dark treatment.
CC {ECO:0000269|PubMed:11124253, ECO:0000269|PubMed:9678973}.
CC -!- SIMILARITY: Belongs to the importin alpha family. {ECO:0000305}.
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DR EMBL; AB004660; BAA31165.1; -; mRNA.
DR EMBL; AF005265; AAQ13406.1; -; mRNA.
DR EMBL; AB004814; BAA31166.1; -; Genomic_DNA.
DR EMBL; AP000816; BAA87855.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS71366.1; -; Genomic_DNA.
DR RefSeq; XP_015621115.1; XM_015765629.1.
DR PDB; 2YNS; X-ray; 2.10 A; A/B=73-526.
DR PDB; 4B8J; X-ray; 2.00 A; A=1-526.
DR PDB; 4B8O; X-ray; 2.08 A; A=73-526.
DR PDB; 4B8P; X-ray; 2.30 A; A/B=73-526.
DR PDB; 4BPL; X-ray; 2.30 A; A=73-526.
DR PDB; 4BQK; X-ray; 2.00 A; A/B=73-526.
DR PDBsum; 2YNS; -.
DR PDBsum; 4B8J; -.
DR PDBsum; 4B8O; -.
DR PDBsum; 4B8P; -.
DR PDBsum; 4BPL; -.
DR PDBsum; 4BQK; -.
DR AlphaFoldDB; Q71VM4; -.
DR SMR; Q71VM4; -.
DR IntAct; Q71VM4; 2.
DR MINT; Q71VM4; -.
DR STRING; 4530.OS01T0253300-01; -.
DR PaxDb; Q71VM4; -.
DR PRIDE; Q71VM4; -.
DR EnsemblPlants; Os01t0253300-01; Os01t0253300-01; Os01g0253300.
DR EnsemblPlants; Os01t0253300-02; Os01t0253300-02; Os01g0253300.
DR GeneID; 4327117; -.
DR Gramene; Os01t0253300-01; Os01t0253300-01; Os01g0253300.
DR Gramene; Os01t0253300-02; Os01t0253300-02; Os01g0253300.
DR KEGG; osa:4327117; -.
DR eggNOG; KOG0166; Eukaryota.
DR HOGENOM; CLU_018084_5_0_1; -.
DR InParanoid; Q71VM4; -.
DR OMA; EMIQMLY; -.
DR OrthoDB; 1111872at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR ExpressionAtlas; Q71VM4; baseline and differential.
DR Genevisible; Q71VM4; OS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0061608; F:nuclear import signal receptor activity; IBA:GO_Central.
DR GO; GO:0008139; F:nuclear localization sequence binding; IBA:GO_Central.
DR GO; GO:0006607; P:NLS-bearing protein import into nucleus; IBA:GO_Central.
DR Gene3D; 1.20.5.690; -; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR032413; Arm_3.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR002652; Importin-a_IBB.
DR InterPro; IPR036975; Importin-a_IBB_sf.
DR InterPro; IPR024931; Importin_alpha.
DR Pfam; PF00514; Arm; 8.
DR Pfam; PF16186; Arm_3; 1.
DR Pfam; PF01749; IBB; 1.
DR PIRSF; PIRSF005673; Importin_alpha; 1.
DR SMART; SM00185; ARM; 8.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 4.
DR PROSITE; PS51214; IBB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Host-virus interaction; Protein transport;
KW Reference proteome; Repeat; Transport.
FT CHAIN 1..526
FT /note="Importin subunit alpha-1a"
FT /id="PRO_0000120740"
FT DOMAIN 1..58
FT /note="IBB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00561"
FT REPEAT 105..145
FT /note="ARM 1"
FT REPEAT 148..187
FT /note="ARM 2"
FT REPEAT 190..230
FT /note="ARM 3"
FT REPEAT 232..271
FT /note="ARM 4"
FT REPEAT 274..313
FT /note="ARM 5"
FT REPEAT 316..356
FT /note="ARM 6"
FT REPEAT 359..398
FT /note="ARM 7"
FT REPEAT 402..441
FT /note="ARM 8"
FT CONFLICT 473..474
FT /note="LQ -> FE (in Ref. 2; AAQ13406)"
FT /evidence="ECO:0000305"
FT HELIX 74..82
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 86..100
FT /evidence="ECO:0007829|PDB:4BQK"
FT STRAND 103..105
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 108..113
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 117..123
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 130..144
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 148..156
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 159..166
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 172..186
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 190..198
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 202..207
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 215..229
FT /evidence="ECO:0007829|PDB:4BQK"
FT STRAND 231..233
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 237..240
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 243..250
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 256..269
FT /evidence="ECO:0007829|PDB:4BQK"
FT STRAND 271..273
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 274..282
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 286..292
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 298..311
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 316..323
FT /evidence="ECO:0007829|PDB:4BQK"
FT TURN 324..326
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 327..336
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 341..355
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 359..368
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 371..380
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 383..399
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 402..410
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 414..419
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 420..422
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 426..449
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 456..463
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 466..473
FT /evidence="ECO:0007829|PDB:4BQK"
FT HELIX 479..492
FT /evidence="ECO:0007829|PDB:4BQK"
SQ SEQUENCE 526 AA; 57571 MW; 7A7DA264968FC983 CRC64;
MSLRPSERVE VRRNRYKVAV DAEEGRRRRE DNMVEIRKSR REESLLKKRR EGLQAQAPVP
ASAATGVDKK LESLPAMIGG VYSDDNNLQL EATTQFRKLL SIERSPPIEE VIQSGVVPRF
VQFLTREDFP QLQFEAAWAL TNIASGTSEN TKVVIDHGAV PIFVKLLGSS SDDVREQAVW
ALGNVAGDSP KCRDLVLANG ALLPLLAQLN EHTKLSMLRN ATWTLSNFCR GKPQPSFEQT
RPALPALARL IHSNDEEVLT DACWALSYLS DGTNDKIQAV IEAGVCPRLV ELLLHPSPSV
LIPALRTVGN IVTGDDAQTQ CIIDHQALPC LLSLLTQNLK KSIKKEACWT ISNITAGNKD
QIQAVINAGI IGPLVNLLQT AEFDIKKEAA WAISNATSGG SHDQIKYLVS EGCIKPLCDL
LICPDIRIVT VCLEGLENIL KVGETDKTLA AGDVNVFSQM IDEAEGLEKI ENLQSHDNNE
IYEKAVKILE AYWMDEEDDT MGATTVAAPQ GATFDFGQGG GAAQFK
