IMA2_CAEEL
ID IMA2_CAEEL Reviewed; 531 AA.
AC P91276;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Importin subunit alpha-2;
DE AltName: Full=Karyopherin subunit alpha-2;
GN Name=ima-2 {ECO:0000303|PubMed:12221121, ECO:0000312|WormBase:F26B1.3};
GN ORFNames=F26B1.3;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|EMBL:AAG49386.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=11311162; DOI=10.1242/dev.128.10.1817;
RA Geles K.G., Adam S.A.;
RT "Germline and developmental roles of the nuclear transport factor importin
RT alpha3 in C. elegans.";
RL Development 128:1817-1830(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Bristol N2;
RA Kohara Y., Shin-i T., Suzuki Y., Sugano S., Potdevin M., Thierry-Mieg Y.,
RA Thierry-Mieg D., Thierry-Mieg J.;
RT "The Caenorhabditis elegans transcriptome project, a complementary view of
RT the genome.";
RL Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=12221121; DOI=10.1091/mbc.e02-02-0069;
RA Geles K.G., Johnson J.J., Jong S., Adam S.A.;
RT "A role for Caenorhabditis elegans importin IMA-2 in germ line and
RT embryonic mitosis.";
RL Mol. Biol. Cell 13:3138-3147(2002).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND INTERACTION WITH
RP AKIR-1.
RX PubMed=30563860; DOI=10.1534/genetics.118.301458;
RA Bowman R., Balukof N., Ford T., Smolikove S.;
RT "A novel role for alpha-importins and Akirin in establishment of meiotic
RT sister chromatid cohesion in Caenorhabditis elegans.";
RL Genetics 211:617-635(2019).
CC -!- FUNCTION: Nuclear transport receptor that mediates nuclear import of
CC proteins, and which is involved in sister chromatid cohesion
CC (PubMed:11311162, PubMed:12221121, PubMed:30563860). Binds specifically
CC and directly to substrates containing either a simple or bipartite
CC nuclear localization signals (NLS) motif (By similarity). Promotes
CC docking of import substrates to the nuclear envelope (By similarity).
CC Together with akir-1 adapter, required for the import and load of
CC cohesin complex proteins in meiotic nuclei (PubMed:30563860).
CC {ECO:0000250|UniProtKB:Q19969, ECO:0000269|PubMed:11311162,
CC ECO:0000269|PubMed:12221121, ECO:0000269|PubMed:30563860}.
CC -!- SUBUNIT: Forms a complex with an importin beta subunit
CC (PubMed:11311162). Interacts with akir-1 (PubMed:30563860).
CC {ECO:0000269|PubMed:11311162, ECO:0000269|PubMed:30563860}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11311162,
CC ECO:0000269|PubMed:12221121}. Nucleus {ECO:0000269|PubMed:11311162,
CC ECO:0000269|PubMed:12221121, ECO:0000269|PubMed:30563860}. Nucleus
CC envelope {ECO:0000269|PubMed:12221121}. Note=In interphase germ cells
CC and embryonic cells, localizes to the cytoplasm and nuclear envelope,
CC whereas in developing oocytes, it localizes in the cytoplasm and
CC nucleus. {ECO:0000269|PubMed:12221121}.
CC -!- TISSUE SPECIFICITY: Germline tissues (PubMed:11311162,
CC PubMed:12221121). Expressed exclusively in germ line cells from the
CC early embryonic through adult stages (PubMed:12221121).
CC {ECO:0000269|PubMed:11311162, ECO:0000269|PubMed:12221121}.
CC -!- DEVELOPMENTAL STAGE: Expressed weakly in early larvae, levels of
CC expression increase in larval stage 4 and adult stages when germ cells
CC are continually proliferating. {ECO:0000269|PubMed:11311162}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality associated with a terminal
CC aneuploid phenotype: embryos display severe defects in nuclear envelope
CC formation, accumulating nucleoporins and lamin in the cytoplasm
CC (PubMed:12221121). Worms lacking both akir-1 and ima-2 show reduced
CC gonad size and aberrant diakinesis oocyte formation; defects are caused
CC by impaired meiotic recombination (PubMed:30563860).
CC {ECO:0000269|PubMed:12221121, ECO:0000269|PubMed:30563860}.
CC -!- SIMILARITY: Belongs to the importin alpha family. {ECO:0000305}.
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DR EMBL; AF040996; AAB97172.1; -; mRNA.
DR EMBL; FO080500; CCD64196.1; -; Genomic_DNA.
DR EMBL; AF326936; AAG49386.1; -; mRNA.
DR PIR; T30167; T30167.
DR RefSeq; NP_491824.1; NM_059423.5.
DR AlphaFoldDB; P91276; -.
DR SMR; P91276; -.
DR BioGRID; 37782; 9.
DR DIP; DIP-26785N; -.
DR IntAct; P91276; 3.
DR STRING; 6239.F26B1.3; -.
DR iPTMnet; P91276; -.
DR EPD; P91276; -.
DR PaxDb; P91276; -.
DR PeptideAtlas; P91276; -.
DR PRIDE; P91276; -.
DR EnsemblMetazoa; F26B1.3.1; F26B1.3.1; WBGene00002073.
DR GeneID; 172329; -.
DR KEGG; cel:CELE_F26B1.3; -.
DR UCSC; F26B1.3.1; c. elegans.
DR CTD; 172329; -.
DR WormBase; F26B1.3; CE09675; WBGene00002073; ima-2.
DR eggNOG; KOG0166; Eukaryota.
DR GeneTree; ENSGT01050000244891; -.
DR HOGENOM; CLU_018084_6_0_1; -.
DR InParanoid; P91276; -.
DR OMA; VKECCWL; -.
DR OrthoDB; 1111872at2759; -.
DR PhylomeDB; P91276; -.
DR SignaLink; P91276; -.
DR PRO; PR:P91276; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00002073; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005635; C:nuclear envelope; IDA:WormBase.
DR GO; GO:0005643; C:nuclear pore; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0061608; F:nuclear import signal receptor activity; IMP:UniProtKB.
DR GO; GO:0008139; F:nuclear localization sequence binding; IBA:GO_Central.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0051177; P:meiotic sister chromatid cohesion; IMP:UniProtKB.
DR GO; GO:0007084; P:mitotic nuclear membrane reassembly; IMP:WormBase.
DR GO; GO:0006607; P:NLS-bearing protein import into nucleus; IBA:GO_Central.
DR GO; GO:0006606; P:protein import into nucleus; IMP:UniProtKB.
DR GO; GO:0051983; P:regulation of chromosome segregation; IMP:WormBase.
DR Gene3D; 1.20.5.690; -; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR032413; Arm_3.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR002652; Importin-a_IBB.
DR InterPro; IPR036975; Importin-a_IBB_sf.
DR InterPro; IPR024931; Importin_alpha.
DR Pfam; PF00514; Arm; 5.
DR Pfam; PF16186; Arm_3; 1.
DR Pfam; PF01749; IBB; 1.
DR PIRSF; PIRSF005673; Importin_alpha; 1.
DR SMART; SM00185; ARM; 8.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 1.
DR PROSITE; PS51214; IBB; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Meiosis; Nucleus; Protein transport; Reference proteome; Repeat;
KW Transport.
FT CHAIN 1..531
FT /note="Importin subunit alpha-2"
FT /id="PRO_0000120735"
FT DOMAIN 5..67
FT /note="IBB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00561"
FT REPEAT 122..161
FT /note="ARM 1"
FT /evidence="ECO:0000305"
FT REPEAT 164..203
FT /note="ARM 2"
FT /evidence="ECO:0000305"
FT REPEAT 250..290
FT /note="ARM 3"
FT /evidence="ECO:0000305"
FT REPEAT 293..331
FT /note="ARM 4"
FT /evidence="ECO:0000305"
FT REPEAT 334..374
FT /note="ARM 5"
FT /evidence="ECO:0000305"
FT REPEAT 377..416
FT /note="ARM 6"
FT /evidence="ECO:0000305"
FT REPEAT 420..459
FT /note="ARM 7"
FT /evidence="ECO:0000305"
FT REGION 1..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 511..531
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..65
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 531 AA; 59176 MW; 0CB7CEB45E67A114 CRC64;
MTLTETSLSH NAEEGKDEGG RLQQYKNLTK HEELRRRRTE CSVEIRKQKG ADMMMKRRNI
VDVDEGGNSE SELEEPEKIS HQQSSTRLSN DEIRAILSNN PSEDDMVRCF ESLRKSLSKT
KNPPIDEVIH CGLLQALVQA LSVENERVQY EAAWALTNIV SGSTEQTIAA VEAGVTIPLI
HLSVHQSAQI SEQALWAVAN IAGDSSQLRD YVIKCHGVEA LMHLMEKVDQ LGDSHVRTIA
WAFSNMCRHK NPHAPLEVLR VLSKGLVKLV QHTDRQVRQD ACWAVSYLTD GPDEQIELAR
ESGVLPHVVA FFKEAENLVA PALRTLGNVA TGNDSLTQAV IDLGSLDEIL PLMEKTRSSS
IVKECCWLVS NIIAGTQKQI QAVLDANLLP VLINVLKSGD HKCQFEASWA LSNLAQGGTN
RQVVAMLEDN VVPALCQALL QTNTDMLNNT LETLYTLMLT VQNGYPHKVD ILHDQVEENG
GLDSLERLQE SQSEQIYTQA YRIITQFFTD DDAGEKESHE NADPQDNKWS F
