ID   IMA5_XENLA              Reviewed;         522 AA.
AC   P52170;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Importin subunit alpha-5;
DE   AltName: Full=Karyopherin subunit alpha-1;
GN   Name=kpna1;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 2-55; 296-332; 342-346
RP   AND 506-522.
RC   TISSUE=Ovary;
RX   PubMed=8001116; DOI=10.1016/0092-8674(94)90067-1;
RA   Goerlich D., Prehn S., Laskey R.A., Hartmann E.;
RT   "Isolation of a protein that is essential for the first step of nuclear
RT   protein import.";
RL   Cell 79:767-778(1994).
RN   [2]
RP   IDENTIFICATION OF IBB DOMAIN.
RX   PubMed=8617226; DOI=10.1002/j.1460-2075.1996.tb00530.x;
RA   Goerlich D., Henklein P., Laskey R.A., Hartmann E.;
RT   "A 41 amino acid motif in importin-alpha confers binding to importin-beta
RT   and hence transit into the nucleus.";
RL   EMBO J. 15:1810-1817(1996).
CC   -!- FUNCTION: Essential for selective protein import into nucleus. Promotes
CC       signal-dependent binding of karyophilic proteins to the nuclear
CC       envelope.
CC   -!- SUBUNIT: Forms a complex with importin subunit beta-1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
CC   -!- DOMAIN: The importin beta binding domain (IBB domain), which is
CC       sufficient for binding importin beta and essential for nuclear protein
CC       import confers import only, but not re-export out of the nucleus.
CC   -!- SIMILARITY: Belongs to the importin alpha family. {ECO:0000305}.
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DR   EMBL; L36339; AAC14195.1; -; mRNA.
DR   PIR; A55194; A55194.
DR   PIR; B55194; B55194.
DR   RefSeq; XP_018092812.1; XM_018237323.1.
DR   RefSeq; XP_018092813.1; XM_018237324.1.
DR   AlphaFoldDB; P52170; -.
DR   SMR; P52170; -.
DR   IntAct; P52170; 1.
DR   MINT; P52170; -.
DR   DNASU; 398026; -.
DR   GeneID; 398026; -.
DR   CTD; 398026; -.
DR   Xenbase; XB-GENE-5991815; kpna7.S.
DR   OMA; DKRMSKF; -.
DR   OrthoDB; 1111872at2759; -.
DR   Proteomes; UP000186698; Chromosome 9_10S.
DR   Bgee; 398026; Expressed in ovary and 7 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0061608; F:nuclear import signal receptor activity; IEA:InterPro.
DR   GO; GO:0006606; P:protein import into nucleus; IEA:InterPro.
DR   Gene3D; 1.20.5.690; -; 1.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR032413; Arm_3.
DR   InterPro; IPR000225; Armadillo.
DR   InterPro; IPR002652; Importin-a_IBB.
DR   InterPro; IPR036975; Importin-a_IBB_sf.
DR   InterPro; IPR024931; Importin_alpha.
DR   Pfam; PF00514; Arm; 8.
DR   Pfam; PF16186; Arm_3; 1.
DR   Pfam; PF01749; IBB; 1.
DR   PIRSF; PIRSF005673; Importin_alpha; 1.
DR   SMART; SM00185; ARM; 8.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS50176; ARM_REPEAT; 3.
DR   PROSITE; PS51214; IBB; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Nucleus; Protein transport;
KW   Reference proteome; Repeat; Transport.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8001116"
FT   CHAIN           2..522
FT                   /note="Importin subunit alpha-5"
FT                   /id="PRO_0000120731"
FT   DOMAIN          2..57
FT                   /note="IBB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00561"
FT   REPEAT          109..151
FT                   /note="ARM 1"
FT   REPEAT          152..196
FT                   /note="ARM 2"
FT   REPEAT          197..235
FT                   /note="ARM 3"
FT   REPEAT          236..280
FT                   /note="ARM 4"
FT   REPEAT          281..320
FT                   /note="ARM 5"
FT   REPEAT          321..362
FT                   /note="ARM 6"
FT   REPEAT          363..402
FT                   /note="ARM 7"
FT   REPEAT          403..447
FT                   /note="ARM 8"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          235..427
FT                   /note="Binding to RAG1"
FT                   /evidence="ECO:0000250"
FT   MOTIF           23..49
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        309
FT                   /note="T -> P (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   522 AA;  57688 MW;  217030FEAD2C8D02 CRC64;
     MPTTNEADER MRKFKNKGKD TAELRRRRVE VSVELRKAKK DEQILKRRNV CLPEELILSP
     EKNAMQSVQV PPLSLEEIVQ GMNSGDPENE LRCTQAARKM LSRERNPPLN DIIEAGLIPK
     LVEFLSRHDN STLQFEAAWA LTNIASGTSD QTKSVVDGGA IPAFISLISS PHLHISEQAV
     WALGNIAGDG PLYRDALINC NVIPPLLALV NPQTPLGYLR NITWMLSNLC RNKNPYPPMS
     AVLQILPVLT QLMHHDDKDI LSDTCWAMSY LTDGSNDRID VVVKTGIVDR LIQLMYSPEL
     SIVTPSLRTV GNIVTGTDKQ TQAAIDAGVL SVLPQLLRHQ KPSIQKEAAW AISNIAAGPA
     PQIQQMITCG LLSPLVDLLN KGDFKAQKEA VWAVTNYTSG GTVEQVVQLV QCGVLEPLLN
     LLTIKDSKTI LVILDAISNI FLAAEKLGEQ EKLCLLVEEL GGLEKIEALQ THDNHMVYHA
     ALALIEKYFS GEEADDIALE PEMGKDAYTF QVPNMQKESF NF