IMA8_BOVIN
ID IMA8_BOVIN Reviewed; 522 AA.
AC C1JZ66; F1MPD2;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 2.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Importin subunit alpha-8;
DE AltName: Full=Karyopherin subunit alpha-7;
GN Name=KPNA7;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH NPM2, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Embryonic ovary;
RX PubMed=19420384; DOI=10.1095/biolreprod.109.077396;
RA Tejomurtula J., Lee K.B., Tripurani S.K., Smith G.W., Yao J.;
RT "Role of importin alpha8, a new member of the importin alpha family of
RT nuclear transport proteins, in early embryonic development in cattle.";
RL Biol. Reprod. 81:333-342(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford;
RX PubMed=19390049; DOI=10.1126/science.1169588;
RG The bovine genome sequencing and analysis consortium;
RT "The genome sequence of taurine cattle: a window to ruminant biology and
RT evolution.";
RL Science 324:522-528(2009).
CC -!- FUNCTION: Functions in nuclear protein import. {ECO:0000250}.
CC -!- SUBUNIT: Binds to importin subunit beta-1/KPNB1 via the IBB domain;
CC this complex dissociates in the presence of RAN-GTP (By similarity).
CC Binds to the NPM2 nuclear localization signal. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed almost exclusively in ovary, in germinal
CC vesicle (GV)-stage oocytes, but not in granulosa, nor theca cells.
CC Detected at very low levels in testis. {ECO:0000269|PubMed:19420384}.
CC -!- DEVELOPMENTAL STAGE: Expressed at high levels in GV- and MII-stage
CC oocytes, as well as in 2-cell embryos. Levels drop sharply by the
CC blastocyst stage (at protein level). Not detectable in fetal ovaries 90
CC and 95 days of gestation, but highly abundant in fetal ovaries of late
CC gestation. {ECO:0000269|PubMed:19420384}.
CC -!- SIMILARITY: Belongs to the importin alpha family. {ECO:0000305}.
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DR EMBL; FJ754641; ACO50696.1; -; mRNA.
DR EMBL; AAFC03113471; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001157419.1; NM_001163947.1.
DR AlphaFoldDB; C1JZ66; -.
DR SMR; C1JZ66; -.
DR STRING; 9913.ENSBTAP00000020894; -.
DR PaxDb; C1JZ66; -.
DR PRIDE; C1JZ66; -.
DR Ensembl; ENSBTAT00000020894; ENSBTAP00000020894; ENSBTAG00000015737.
DR GeneID; 789280; -.
DR KEGG; bta:789280; -.
DR CTD; 402569; -.
DR VEuPathDB; HostDB:ENSBTAG00000015737; -.
DR VGNC; VGNC:30706; KPNA7.
DR eggNOG; KOG0166; Eukaryota.
DR GeneTree; ENSGT01050000244891; -.
DR HOGENOM; CLU_018084_6_0_1; -.
DR InParanoid; C1JZ66; -.
DR OMA; KENMCLL; -.
DR OrthoDB; 1111872at2759; -.
DR TreeFam; TF101178; -.
DR Proteomes; UP000009136; Chromosome 25.
DR Bgee; ENSBTAG00000015737; Expressed in oocyte and 14 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0001674; C:female germ cell nucleus; IEA:Ensembl.
DR GO; GO:0042564; C:NLS-dependent protein nuclear import complex; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005819; C:spindle; IEA:Ensembl.
DR GO; GO:0061608; F:nuclear import signal receptor activity; IBA:GO_Central.
DR GO; GO:0008139; F:nuclear localization sequence binding; IBA:GO_Central.
DR GO; GO:0001824; P:blastocyst development; IEA:Ensembl.
DR GO; GO:0010629; P:negative regulation of gene expression; IEA:Ensembl.
DR GO; GO:0006607; P:NLS-bearing protein import into nucleus; IBA:GO_Central.
DR GO; GO:0010628; P:positive regulation of gene expression; IEA:Ensembl.
DR GO; GO:1902466; P:positive regulation of histone H3-K27 trimethylation; IEA:Ensembl.
DR Gene3D; 1.20.5.690; -; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR032413; Arm_3.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR002652; Importin-a_IBB.
DR InterPro; IPR036975; Importin-a_IBB_sf.
DR InterPro; IPR024931; Importin_alpha.
DR InterPro; IPR004155; PBS_lyase_HEAT.
DR Pfam; PF00514; Arm; 6.
DR Pfam; PF16186; Arm_3; 1.
DR Pfam; PF01749; IBB; 1.
DR PIRSF; PIRSF005673; Importin_alpha; 1.
DR SMART; SM00185; ARM; 8.
DR SMART; SM00567; EZ_HEAT; 4.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 3.
DR PROSITE; PS51214; IBB; 1.
PE 1: Evidence at protein level;
KW Nucleus; Protein transport; Reference proteome; Repeat; Transport.
FT CHAIN 1..522
FT /note="Importin subunit alpha-8"
FT /id="PRO_0000413536"
FT DOMAIN 1..57
FT /note="IBB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00561"
FT REPEAT 100..140
FT /note="ARM 1"
FT REPEAT 143..182
FT /note="ARM 2"
FT REPEAT 185..225
FT /note="ARM 3"
FT REPEAT 228..267
FT /note="ARM 4"
FT REPEAT 270..309
FT /note="ARM 5"
FT REPEAT 312..351
FT /note="ARM 6"
FT REPEAT 354..393
FT /note="ARM 7"
FT REPEAT 397..436
FT /note="ARM 8"
FT CONFLICT 71
FT /note="E -> G (in Ref. 1; ACO50696)"
FT /evidence="ECO:0000305"
FT CONFLICT 189
FT /note="D -> N (in Ref. 1; ACO50696)"
FT /evidence="ECO:0000305"
FT CONFLICT 193
FT /note="A -> T (in Ref. 1; ACO50696)"
FT /evidence="ECO:0000305"
FT CONFLICT 493
FT /note="F -> L (in Ref. 1; ACO50696)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 522 AA; 57362 MW; 09EDF7D4F2E6D1F4 CRC64;
MPTLDAPEER LRKFKYRGKD ASARRQQRIA VSLELRKAKK DEQALKRRNI TDVSLDPSDQ
QTKGVSLTLQ EIISGVNASD PELCFQATQA ARKMLSREKN PPLKLIVDAG LIPRLVELLK
SSLHPRLQFE AAWALTNIAS GASELTRAVV VGGAIQPLVE LLSSPHMTVC EQAVWALGNI
AGDGPEFRDN VIASDAIPHL LTLVSSSIPV PFLRNIAWTL SNLCRNKNPY PSDHAVKQML
PALFYLLGHP DREVLSDTCW ALSYLTDGCD ARIGQVVDTG VLPRLVELMS SSELNILTPS
LRTVGNIVTG TDHQTQLALD AGILGVLPQL LTHPRPSIQK EAAWALSNVA AGPRQHIQRL
IACGALPPLV AVLKNGEFKV QKEAVWTVAN FTTGGSVEQL IQLVQAGVLE PLINLLTIPD
NKMVIIILDV LFFILQAAEK ISQKENMCLL IEGLGGLDRI EALQLHENHQ VALTALNIIE
RHFSEEEETV TAFRARDQDH KFLKDLTNTY HCCTTPKPGP RP
