IMA8_HUMAN
ID IMA8_HUMAN Reviewed; 516 AA.
AC A9QM74; A4D277;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Importin subunit alpha-8;
DE AltName: Full=Karyopherin subunit alpha-7;
GN Name=KPNA7;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND INTERACTION WITH
RP KPNB1 AND RB1.
RC TISSUE=Prostatic adenocarcinoma;
RX PubMed=20701745; DOI=10.1186/1471-2121-11-63;
RA Kelley J.B., Talley A.M., Spencer A., Gioeli D., Paschal B.M.;
RT "Karyopherin alpha7 (KPNA7), a divergent member of the importin alpha
RT family of nuclear import receptors.";
RL BMC Cell Biol. 11:63-63(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
CC -!- FUNCTION: Functions in nuclear protein import. {ECO:0000250}.
CC -!- SUBUNIT: Binds very efficiently to importin subunit beta-1/KPNB1 via
CC the IBB domain; this complex dissociates in the presence of RAN-GTP.
CC Shows a limited binding to the RB1 nuclear localization signal (NLS),
CC but not to the SV40, nor NPM1 NLSs.
CC -!- INTERACTION:
CC A9QM74; Q14974: KPNB1; NbExp=2; IntAct=EBI-13942676, EBI-286758;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20701745}.
CC -!- SIMILARITY: Belongs to the importin alpha family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAL23884.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; EU126604; ABW96272.1; -; mRNA.
DR EMBL; CH236956; EAL23884.1; ALT_INIT; Genomic_DNA.
DR CCDS; CCDS47651.1; -.
DR RefSeq; NP_001139187.1; NM_001145715.2.
DR AlphaFoldDB; A9QM74; -.
DR SMR; A9QM74; -.
DR BioGRID; 135514; 7.
DR ComplexPortal; CPX-1066; Importin complex, KPNA7 variant.
DR IntAct; A9QM74; 1.
DR STRING; 9606.ENSP00000330878; -.
DR GlyGen; A9QM74; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; A9QM74; -.
DR PhosphoSitePlus; A9QM74; -.
DR BioMuta; KPNA7; -.
DR jPOST; A9QM74; -.
DR MassIVE; A9QM74; -.
DR MaxQB; A9QM74; -.
DR PaxDb; A9QM74; -.
DR PeptideAtlas; A9QM74; -.
DR PRIDE; A9QM74; -.
DR ProteomicsDB; 2499; -.
DR Antibodypedia; 30296; 43 antibodies from 19 providers.
DR DNASU; 402569; -.
DR Ensembl; ENST00000327442.7; ENSP00000330878.6; ENSG00000185467.8.
DR Ensembl; ENST00000640158.1; ENSP00000491331.1; ENSG00000284068.1.
DR Ensembl; ENST00000681060.1; ENSP00000506489.1; ENSG00000185467.8.
DR GeneID; 402569; -.
DR KEGG; hsa:402569; -.
DR MANE-Select; ENST00000327442.7; ENSP00000330878.6; NM_001145715.3; NP_001139187.1.
DR UCSC; uc010lft.3; human.
DR CTD; 402569; -.
DR DisGeNET; 402569; -.
DR GeneCards; KPNA7; -.
DR HGNC; HGNC:21839; KPNA7.
DR HPA; ENSG00000185467; Tissue enriched (stomach).
DR MalaCards; KPNA7; -.
DR MIM; 614107; gene.
DR neXtProt; NX_A9QM74; -.
DR OpenTargets; ENSG00000185467; -.
DR Orphanet; 401959; Partial corpus callosum agenesis-cerebellar vermis hypoplasia with posterior fossa cysts syndrome.
DR PharmGKB; PA164722048; -.
DR VEuPathDB; HostDB:ENSG00000185467; -.
DR eggNOG; KOG0166; Eukaryota.
DR GeneTree; ENSGT01050000244891; -.
DR HOGENOM; CLU_018084_6_0_1; -.
DR InParanoid; A9QM74; -.
DR OMA; KENMCLL; -.
DR OrthoDB; 1111872at2759; -.
DR PhylomeDB; A9QM74; -.
DR TreeFam; TF101178; -.
DR PathwayCommons; A9QM74; -.
DR Reactome; R-HSA-1169408; ISG15 antiviral mechanism.
DR Reactome; R-HSA-168276; NS1 Mediated Effects on Host Pathways.
DR SignaLink; A9QM74; -.
DR BioGRID-ORCS; 402569; 14 hits in 1064 CRISPR screens.
DR ChiTaRS; KPNA7; human.
DR GenomeRNAi; 402569; -.
DR Pharos; A9QM74; Tbio.
DR PRO; PR:A9QM74; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; A9QM74; protein.
DR Bgee; ENSG00000185467; Expressed in olfactory segment of nasal mucosa and 40 other tissues.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0001674; C:female germ cell nucleus; IEA:Ensembl.
DR GO; GO:0042564; C:NLS-dependent protein nuclear import complex; IPI:ComplexPortal.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005819; C:spindle; IEA:Ensembl.
DR GO; GO:0061608; F:nuclear import signal receptor activity; IBA:GO_Central.
DR GO; GO:0008139; F:nuclear localization sequence binding; IBA:GO_Central.
DR GO; GO:0001824; P:blastocyst development; IEA:Ensembl.
DR GO; GO:0010629; P:negative regulation of gene expression; IEA:Ensembl.
DR GO; GO:0006607; P:NLS-bearing protein import into nucleus; IBA:GO_Central.
DR GO; GO:0010628; P:positive regulation of gene expression; IEA:Ensembl.
DR GO; GO:1902466; P:positive regulation of histone H3-K27 trimethylation; IEA:Ensembl.
DR Gene3D; 1.20.5.690; -; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR032413; Arm_3.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR002652; Importin-a_IBB.
DR InterPro; IPR036975; Importin-a_IBB_sf.
DR InterPro; IPR024931; Importin_alpha.
DR Pfam; PF00514; Arm; 7.
DR Pfam; PF16186; Arm_3; 1.
DR Pfam; PF01749; IBB; 1.
DR PIRSF; PIRSF005673; Importin_alpha; 1.
DR SMART; SM00185; ARM; 8.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 5.
DR PROSITE; PS51214; IBB; 1.
PE 1: Evidence at protein level;
KW Nucleus; Protein transport; Reference proteome; Repeat; Transport.
FT CHAIN 1..516
FT /note="Importin subunit alpha-8"
FT /id="PRO_0000326082"
FT DOMAIN 1..57
FT /note="IBB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00561"
FT REPEAT 103..143
FT /note="ARM 1"
FT REPEAT 146..185
FT /note="ARM 2"
FT REPEAT 188..228
FT /note="ARM 3"
FT REPEAT 231..270
FT /note="ARM 4"
FT REPEAT 273..312
FT /note="ARM 5"
FT REPEAT 315..354
FT /note="ARM 6"
FT REPEAT 357..396
FT /note="ARM 7"
FT REPEAT 400..439
FT /note="ARM 8"
SQ SEQUENCE 516 AA; 56938 MW; 803D7CC662C09378 CRC64;
MPTLDAPEER RRKFKYRGKD VSLRRQQRMA VSLELRKAKK DEQTLKRRNI TSFCPDTPSE
KTAKGVAVSL TLGEIIKGVN SSDPVLCFQA TQTARKMLSQ EKNPPLKLVI EAGLIPRMVE
FLKSSLYPCL QFEAAWALTN IASGTSEQTR AVVEGGAIQP LIELLSSSNV AVCEQAVWAL
GNIAGDGPEF RDNVITSNAI PHLLALISPT LPITFLRNIT WTLSNLCRNK NPYPCDTAVK
QILPALLHLL QHQDSEVLSD ACWALSYLTD GSNKRIGQVV NTGVLPRLVV LMTSSELNVL
TPSLRTVGNI VTGTDEQTQM AIDAGMLNVL PQLLQHNKPS IQKEAAWALS NVAAGPCHHI
QQLLAYDVLP PLVALLKNGE FKVQKEAVWM VANFATGATM DQLIQLVHSG VLEPLVNLLT
APDVKIVLII LDVISCILQA AEKRSEKENL CLLIEELGGI DRIEALQLHE NRQIGQSALN
IIEKHFGEEE DESQTLLSQV IDQDYEFIDY ECLAKK
