IMA8_MOUSE
ID IMA8_MOUSE Reviewed; 499 AA.
AC C0LLJ0; C0LLJ1; Q3UWY3; Q58HC5;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Importin subunit alpha-8;
DE AltName: Full=Karyopherin subunit alpha-7;
GN Name=Kpna7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION,
RP INTERACTION WITH KPNB1, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=C57BL/6J; TISSUE=Ovary;
RX PubMed=20699224; DOI=10.1074/jbc.m110.117044;
RA Hu J., Wang F., Yuan Y., Zhu X., Wang Y., Zhang Y., Kou Z., Wang S.,
RA Gao S.;
RT "Novel importin-alpha family member Kpna7 is required for normal fertility
RT and fecundity in the mouse.";
RL J. Biol. Chem. 285:33113-33122(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Hartmann E.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 105-499 (ISOFORM 2).
RC TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
CC -!- FUNCTION: Functions in nuclear protein import. {ECO:0000250}.
CC -!- SUBUNIT: Binds very efficiently to importin subunit beta-1/KPNB1 via
CC the IBB domain. This complex dissociates in the presence of RAN-GTP (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20699224}. Note=In
CC MII-stage oocytes, localizes to the spindle.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=C0LLJ0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=C0LLJ0-2; Sequence=VSP_041924;
CC -!- TISSUE SPECIFICITY: Expressed predominantly in ovary. Isoform 1 is the
CC predominant form. {ECO:0000269|PubMed:20699224}.
CC -!- DEVELOPMENTAL STAGE: Expressed at high levels in germinal vesicle-stage
CC oocytes, as well as in zygotes and 2-cell embryos (at protein level).
CC Drastically down-regulated after the 2-cell stage.
CC {ECO:0000269|PubMed:20699224}.
CC -!- DISRUPTION PHENOTYPE: Mutant mice exhibit abnormal preimplantation
CC development. About half of the mutant embryos fail to develop into the
CC blastocyst stage, or are delayed. Lethality is greater among female
CC than among male embryos. {ECO:0000269|PubMed:20699224}.
CC -!- SIMILARITY: Belongs to the importin alpha family. {ECO:0000305}.
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DR EMBL; FJ717332; ACN85341.1; -; mRNA.
DR EMBL; FJ717333; ACN85342.1; -; mRNA.
DR EMBL; AY950703; AAX50192.1; -; mRNA.
DR EMBL; AC110556; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC113295; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466529; EDL19009.1; -; Genomic_DNA.
DR EMBL; AK136027; BAE22781.1; -; mRNA.
DR CCDS; CCDS19852.1; -. [C0LLJ0-1]
DR CCDS; CCDS84996.1; -. [C0LLJ0-2]
DR RefSeq; NP_001013796.2; NM_001013774.2. [C0LLJ0-1]
DR RefSeq; NP_001334460.1; NM_001347531.1. [C0LLJ0-2]
DR RefSeq; XP_017176433.1; XM_017320944.1.
DR AlphaFoldDB; C0LLJ0; -.
DR SMR; C0LLJ0; -.
DR ComplexPortal; CPX-1067; Importin complex, KPNA7 variant.
DR iPTMnet; C0LLJ0; -.
DR PhosphoSitePlus; C0LLJ0; -.
DR PaxDb; C0LLJ0; -.
DR PeptideAtlas; C0LLJ0; -.
DR PRIDE; C0LLJ0; -.
DR Antibodypedia; 30296; 43 antibodies from 19 providers.
DR DNASU; 381686; -.
DR Ensembl; ENSMUST00000110672; ENSMUSP00000106300; ENSMUSG00000038770. [C0LLJ0-1]
DR Ensembl; ENSMUST00000110673; ENSMUSP00000106301; ENSMUSG00000038770. [C0LLJ0-2]
DR Ensembl; ENSMUST00000116454; ENSMUSP00000112155; ENSMUSG00000038770. [C0LLJ0-1]
DR GeneID; 381686; -.
DR KEGG; mmu:381686; -.
DR UCSC; uc009alx.1; mouse. [C0LLJ0-2]
DR UCSC; uc009aly.1; mouse. [C0LLJ0-1]
DR CTD; 402569; -.
DR MGI; MGI:2141165; Kpna7.
DR VEuPathDB; HostDB:ENSMUSG00000038770; -.
DR eggNOG; KOG0166; Eukaryota.
DR GeneTree; ENSGT01050000244891; -.
DR HOGENOM; CLU_018084_6_1_1; -.
DR InParanoid; C0LLJ0; -.
DR OMA; KENMCLL; -.
DR OrthoDB; 1111872at2759; -.
DR PhylomeDB; C0LLJ0; -.
DR TreeFam; TF101178; -.
DR BioGRID-ORCS; 381686; 2 hits in 59 CRISPR screens.
DR ChiTaRS; Kpna7; mouse.
DR PRO; PR:C0LLJ0; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; C0LLJ0; protein.
DR Bgee; ENSMUSG00000038770; Expressed in animal zygote and 20 other tissues.
DR ExpressionAtlas; C0LLJ0; baseline and differential.
DR Genevisible; C0LLJ0; MM.
DR GO; GO:0005829; C:cytosol; IC:ComplexPortal.
DR GO; GO:0001674; C:female germ cell nucleus; IDA:MGI.
DR GO; GO:0042564; C:NLS-dependent protein nuclear import complex; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0005819; C:spindle; IDA:MGI.
DR GO; GO:0061608; F:nuclear import signal receptor activity; IBA:GO_Central.
DR GO; GO:0008139; F:nuclear localization sequence binding; IBA:GO_Central.
DR GO; GO:0001824; P:blastocyst development; IMP:MGI.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:MGI.
DR GO; GO:0006607; P:NLS-bearing protein import into nucleus; IBA:GO_Central.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:MGI.
DR GO; GO:1902466; P:positive regulation of histone H3-K27 trimethylation; IMP:MGI.
DR GO; GO:0006606; P:protein import into nucleus; IC:ComplexPortal.
DR Gene3D; 1.20.5.690; -; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR032413; Arm_3.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR002652; Importin-a_IBB.
DR InterPro; IPR036975; Importin-a_IBB_sf.
DR InterPro; IPR024931; Importin_alpha.
DR Pfam; PF00514; Arm; 5.
DR Pfam; PF16186; Arm_3; 1.
DR Pfam; PF01749; IBB; 1.
DR PIRSF; PIRSF005673; Importin_alpha; 1.
DR SMART; SM00185; ARM; 8.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 2.
DR PROSITE; PS51214; IBB; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Protein transport; Reference proteome;
KW Repeat; Transport.
FT CHAIN 1..499
FT /note="Importin subunit alpha-8"
FT /id="PRO_0000413537"
FT DOMAIN 1..57
FT /note="IBB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00561"
FT REPEAT 101..141
FT /note="ARM 1"
FT REPEAT 144..183
FT /note="ARM 2"
FT REPEAT 186..226
FT /note="ARM 3"
FT REPEAT 229..268
FT /note="ARM 4"
FT REPEAT 271..310
FT /note="ARM 5"
FT REPEAT 313..352
FT /note="ARM 6"
FT REPEAT 354..393
FT /note="ARM 7"
FT REPEAT 397..436
FT /note="ARM 8"
FT VAR_SEQ 182
FT /note="A -> ADRKMPDTQVQIFTPSTREAKA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072,
FT ECO:0000303|PubMed:20699224"
FT /id="VSP_041924"
FT CONFLICT 161
FT /note="E -> D (in Ref. 1; ACN85342 and 2; AAX50192)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 499 AA; 55458 MW; 0B1094E04CAFA556 CRC64;
MATSKAPKER LKNYKYRGKE MSLPRQQRIA SSLQLRKTRK DEQVLKRRNI DLFSSDMVSQ
ALVKEVNFTL DDIIQAVNSS DPILHFRATR AAREMISQEN TPPLNLIIEA GLIPKLVDFL
KATPHPKLQF EAAWVLTNIA SGTSEQTRAV VKEGAIQPLI ELLCSPHLTV SEQAVWALGN
IAGDCAEFRD CVISNNAIPH LINLISKGIP ITFLRNISWT LSNLCRNKDP YPSESAVRQM
LPPLCQLLLH RDNEILADTC WALSYLTKGG KEYIHHVVTT GILPRLVELM TSSELSISIP
CLHTIGNIVA GTDEQTQMAI DAGMLKVLGQ VLKHPKTSIQ VLAAWTMSNV AAGPRHQVEQ
LLCNLLPILV DLLRNAELKV QKEVVCTVIN IATGASQDQL TLLAHSGILE PMLSLLSAPD
LEVVIIVLDI ISYLLQHIDN LQEKKRLYFQ IEKFGGFEKI ECLQHHHNIS ISNSALDIIE
KYFCEDGDGD SLPGPGLRV
