IMAP2_ORYSJ
ID IMAP2_ORYSJ Reviewed; 528 AA.
AC Q9FYP9; O80332; Q0JQJ0;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Importin subunit alpha-2;
GN OrderedLocusNames=Os01g0158000, LOC_Os01g06470; ORFNames=P0011G08.44;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Nipponbare;
RX PubMed=9654145; DOI=10.1016/s0014-5793(98)00540-7;
RA Iwasaki T., Matsuki R., Shoji K., Sanmiya K., Miyao M., Yamamoto N.;
RT "A novel importin alpha from rice, a component involved in the process of
RT nuclear protein transport.";
RL FEBS Lett. 428:259-262(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=11124253; DOI=10.1074/jbc.m006430200;
RA Jiang C.-J., Shoji K., Matsuki R., Baba A., Inagaki N., Ban H., Iwasaki T.,
RA Imamoto N., Yoneda Y., Deng X.-W., Yamamoto N.;
RT "Molecular cloning of a novel importin alpha homologue from rice, by which
RT constitutive photomorphogenic 1 (COP1) nuclear localization signal (NLS)-
RT protein is preferentially nuclear imported.";
RL J. Biol. Chem. 276:9322-9329(2001).
CC -!- FUNCTION: Binds specifically and directly to substrates containing
CC either a simple or bipartite NLS motif. Promotes docking of import
CC substrates to the nuclear envelope. {ECO:0000269|PubMed:11124253}.
CC -!- SUBUNIT: Forms a complex with importin subunit beta-1. The whole
CC complex, most stable and composed of importin alpha, importin beta and
CC NLS substrate, is referred to as PTAC or pore targeting complex.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region.
CC -!- TISSUE SPECIFICITY: Expressed in root, callus, and etiolated leaf. Low
CC expression in green leaf. {ECO:0000269|PubMed:11124253,
CC ECO:0000269|PubMed:9654145}.
CC -!- INDUCTION: By dark in green leaf. Down-regulated by light.
CC {ECO:0000269|PubMed:11124253}.
CC -!- SIMILARITY: Belongs to the importin alpha family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08186.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAB64664.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D78504; BAA31220.1; -; mRNA.
DR EMBL; AB006788; BAA31222.1; -; Genomic_DNA.
DR EMBL; AP002539; BAB08186.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP003225; BAB64664.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008207; BAF03988.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS70498.1; -; Genomic_DNA.
DR EMBL; AK103127; BAG95907.1; -; mRNA.
DR PIR; T02972; T02972.
DR RefSeq; XP_015619230.1; XM_015763744.1.
DR AlphaFoldDB; Q9FYP9; -.
DR SMR; Q9FYP9; -.
DR STRING; 4530.OS01T0158000-01; -.
DR PaxDb; Q9FYP9; -.
DR PRIDE; Q9FYP9; -.
DR EnsemblPlants; Os01t0158000-01; Os01t0158000-01; Os01g0158000.
DR EnsemblPlants; Os01t0158000-02; Os01t0158000-02; Os01g0158000.
DR GeneID; 4324088; -.
DR Gramene; Os01t0158000-01; Os01t0158000-01; Os01g0158000.
DR Gramene; Os01t0158000-02; Os01t0158000-02; Os01g0158000.
DR KEGG; osa:4324088; -.
DR eggNOG; KOG0166; Eukaryota.
DR HOGENOM; CLU_516194_0_0_1; -.
DR InParanoid; Q9FYP9; -.
DR OMA; QMRNMAN; -.
DR OrthoDB; 1401503at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q9FYP9; OS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0061608; F:nuclear import signal receptor activity; IBA:GO_Central.
DR GO; GO:0008139; F:nuclear localization sequence binding; IBA:GO_Central.
DR GO; GO:0006607; P:NLS-bearing protein import into nucleus; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR024931; Importin_alpha.
DR Pfam; PF00514; Arm; 3.
DR PIRSF; PIRSF005673; Importin_alpha; 1.
DR SMART; SM00185; ARM; 6.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Protein transport; Reference proteome; Repeat; Transport.
FT CHAIN 1..528
FT /note="Importin subunit alpha-2"
FT /id="PRO_0000120742"
FT REPEAT 125..165
FT /note="ARM 1"
FT REPEAT 167..206
FT /note="ARM 2"
FT REPEAT 209..248
FT /note="ARM 3"
FT REPEAT 253..292
FT /note="ARM 4"
FT REPEAT 294..335
FT /note="ARM 5"
FT REPEAT 338..383
FT /note="ARM 6"
FT REPEAT 386..425
FT /note="ARM 7"
FT REPEAT 438..477
FT /note="ARM 8"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 231
FT /note="S -> N (in Ref. 1; BAA31220)"
FT /evidence="ECO:0000305"
FT CONFLICT 349
FT /note="H -> N (in Ref. 1; BAA31220)"
FT /evidence="ECO:0000305"
FT CONFLICT 408
FT /note="M -> A (in Ref. 1; BAA31220)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 528 AA; 57186 MW; 0B7885BBE87F55B8 CRC64;
MADDSASPSP SSASPLQHHR EALKSSVRNT AASRRREQAI AIGKERREAL IRAKRVCRAP
ISGSDEAEME EGDMVVDEEK ACLEAKTAHA VEELKSALSI QGKGVQKKKI EALRDLRRLL
SQPEVPLVDT AIKAGAVPLL VQYLSFGSSD EQLLEAAWCL TNIAAGEPEE TKSLLPALPL
LIAHLGEKSS TLVAEQCAWA IGNVAGEGAE LRSTLLAQGA LRPLTRLMFS SKGSTARTAA
WAMSNLIKGP DPKAANELIT IDGVLNAIIA SLEKEDEELA TEVAWVVVYL SALSDRGISL
IVRSSVPQLL IGRLFSSENL QLLIPVLRGL GNLIAADDYM VDSVLTVGHN IIDQALSGLI
KCLKSDNRVL RKESSWALSN IAAGSFEHKK LIFASEATPV LIRLVTSMQF DIRREAAYTL
GNLCVVPTGN CELPKIIVEH LVAIVDGGAL PGFIHLVRSA DVDTAGLGLQ FLELVMRGYP
NKQGPKLVEM EDGIEAMERF QFHENEQMRN MANGLVDEYF GEDYGLDE
