ID   IMB4_SCHPO              Reviewed;        1067 AA.
AC   O60100; Q9US72;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 150.
DE   RecName: Full=Probable importin subunit beta-4;
DE   AltName: Full=Importin-123;
DE   AltName: Full=Karyopherin subunit beta-4;
DE   AltName: Full=Karyopherin-123;
GN   Name=kap123; ORFNames=SPBC14F5.03c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 372-605, AND SUBCELLULAR
RP   LOCATION.
RC   STRAIN=ATCC 38364 / 968;
RX   PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA   Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA   Hiraoka Y.;
RT   "Large-scale screening of intracellular protein localization in living
RT   fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL   Genes Cells 5:169-190(2000).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- FUNCTION: Required for nuclear protein import, its predominant
CC       substrate seems to be ribosomal proteins. Binds to nucleoporins and the
CC       GTP-bound form of gsp1 (Ran) (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus. Nucleus
CC       envelope.
CC   -!- SIMILARITY: Belongs to the importin beta family. {ECO:0000305}.
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DR   EMBL; CU329671; CAA19321.1; -; Genomic_DNA.
DR   EMBL; AB028002; BAA87306.1; -; Genomic_DNA.
DR   PIR; T39449; T39449.
DR   RefSeq; NP_596729.1; NM_001022655.2.
DR   AlphaFoldDB; O60100; -.
DR   SMR; O60100; -.
DR   BioGRID; 276410; 10.
DR   DIP; DIP-38714N; -.
DR   IntAct; O60100; 2.
DR   STRING; 4896.SPBC14F5.03c.1; -.
DR   iPTMnet; O60100; -.
DR   MaxQB; O60100; -.
DR   PaxDb; O60100; -.
DR   PRIDE; O60100; -.
DR   EnsemblFungi; SPBC14F5.03c.1; SPBC14F5.03c.1:pep; SPBC14F5.03c.
DR   GeneID; 2539863; -.
DR   KEGG; spo:SPBC14F5.03c; -.
DR   PomBase; SPBC14F5.03c; kap123.
DR   VEuPathDB; FungiDB:SPBC14F5.03c; -.
DR   eggNOG; KOG2171; Eukaryota.
DR   HOGENOM; CLU_003794_1_1_1; -.
DR   InParanoid; O60100; -.
DR   OMA; VRGNAAY; -.
DR   PhylomeDB; O60100; -.
DR   PRO; PR:O60100; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005635; C:nuclear envelope; HDA:PomBase.
DR   GO; GO:0034399; C:nuclear periphery; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0061608; F:nuclear import signal receptor activity; ISO:PomBase.
DR   GO; GO:0008139; F:nuclear localization sequence binding; IBA:GO_Central.
DR   GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR   GO; GO:0006606; P:protein import into nucleus; ISO:PomBase.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR021133; HEAT_type_2.
DR   InterPro; IPR001494; Importin-beta_N.
DR   InterPro; IPR040122; Importin_beta.
DR   PANTHER; PTHR10527; PTHR10527; 1.
DR   Pfam; PF03810; IBN_N; 1.
DR   SMART; SM00913; IBN_N; 1.
DR   SUPFAM; SSF48371; SSF48371; 2.
DR   PROSITE; PS50077; HEAT_REPEAT; 2.
DR   PROSITE; PS50166; IMPORTIN_B_NT; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Nucleus; Protein transport; Reference proteome; Repeat;
KW   Transport.
FT   CHAIN           1..1067
FT                   /note="Probable importin subunit beta-4"
FT                   /id="PRO_0000120775"
FT   DOMAIN          27..94
FT                   /note="Importin N-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00115"
FT   REPEAT          159..196
FT                   /note="HEAT 1"
FT   REPEAT          379..416
FT                   /note="HEAT 2"
FT   REPEAT          420..457
FT                   /note="HEAT 3"
FT   REPEAT          591..633
FT                   /note="HEAT 4"
FT   REPEAT          890..927
FT                   /note="HEAT 5"
FT   REPEAT          1013..1050
FT                   /note="HEAT 6"
SQ   SEQUENCE   1067 AA;  117834 MW;  0D90A3CB4DE13BB6 CRC64;
     MDAQFTLELT QLLFQSIAPD TTQITEATRA LETKYLKEPG SLLSLFHIMG TCENPQVRQL
     AAIEARKLCH KYWSSVDADV QNQIRSNLLD ITLKEPESIV RHAFGRVIAA LAKLDLPEGK
     WNELSAFLVQ ATMDQNDSIR EMAVYVLYSI AETVDLDNKL LLDFVNLFSQ TITDSSRTVR
     VTSVQGLGAI AEVLESDDKK LLHAYRATLP GMLLVLQDVV QVGDVDASKQ VFDVFNTFLI
     ASGAIISKAL GNIIEIITGI ANSKQVDDEI RCMALSFIIS CIRFKSRKLQ ALKLGKPLVL
     TLMEVATEET TDDIDEDCPA RLALRSIDLL STHLSPSQVF YPMFEAACAF SQSPQASYRK
     AALLSIGVAV EGSSESVAGN LPNIFPIIIN GLCDNDMDVR QAALLALSQI AVEIPTEVSK
     HHAQLLPLVF ELMSTQGVKV GKSACNCIDA LLEGLDKSEI SGYLPMLMER LVGLLEFSDT
     PDIKSCVAAA IGSAAFAAQD DFIPYFERTM ASLSQCLHTT DDDEGYELRG TVMDTLGAIA
     NAVGKQAFLP YTEQLIQLAY EGIQIDHSRL RECSFCFYAV LARVYKEEFA PFLEHIVPAL
     FKSIDQDESD ILSERIGAPT AEEISQLLDS VETNEEENDE ELEKAMGVNS AIAMEKEIAA
     DALGEICMYV GAPFTPYLEP TVEKLVACTT HFYEGVRKSA LSSLWRCATT YYKVCNVPQW
     QPGLPLKVPV PDTVKNIFEA VRKCTFDTLE EEYEKTVATD ILRNFAESIK TCGPVVLGDD
     YEKLCEVVME VLQKQHIVQA GDVFDDDFEE EDIVSNEEVD DTEQDALLID SACDVVIALA
     VALGGSFADS FKVFYPQIVK YYMSKNGNER AMAVACVGEV AGGIESAITP FTRDVFSLFM
     AALEDSEGEV RSNAAYSMGL LCQFSTEDLS SEYLNILQKL QPFFTQEVFR TALDNAIGCI
     SRLILHNQNA IPVDQVLPIV FSKLPLKEDY LENAPLYHMI LALYRQQNPC LVQHLGELIP
     VFASVLTGSP EQLNDELRSE LLSMVKEIAP QYESVVSNYP QLVALLQ