IMB_DROME
ID IMB_DROME Reviewed; 884 AA.
AC O18388; Q540Y0; Q9V3E6;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 178.
DE RecName: Full=Importin subunit beta;
DE AltName: Full=Karyopherin subunit beta;
DE AltName: Full=Protein ketel;
GN Name=Fs(2)Ket; ORFNames=CG2637;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=11102382; DOI=10.1093/genetics/156.4.1889;
RA Lippai M., Tirian L., Boros I., Mihaly J., Erdelyi M., Belecz I., Mathe E.,
RA Posfai J., Nagy A., Udvardy A., Paraskeva E., Goerlich D., Szabad J.;
RT "The Ketel gene encodes a Drosophila homologue of importin-beta.";
RL Genetics 156:1889-1900(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Goerlich D., Hartmann E.;
RT "Drosophila melanogaster importin beta.";
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=23885126; DOI=10.1091/mbc.e13-03-0118;
RA Natalizio A.H., Matera A.G.;
RT "Identification and characterization of Drosophila Snurportin reveals a
RT role for the import receptor Moleskin/Importin7 in snRNP biogenesis.";
RL Mol. Biol. Cell 24:2932-2942(2013).
RN [7]
RP INTERACTION WITH MET.
RX PubMed=27979731; DOI=10.1016/j.ibmb.2016.12.005;
RA He Q., Zhang Y., Zhang X., Xu D., Dong W., Li S., Wu R.;
RT "Nucleoporin Nup358 facilitates nuclear import of Methoprene-tolerant (Met)
RT in an importin beta- and Hsp83-dependent manner.";
RL Insect Biochem. Mol. Biol. 81:10-18(2017).
CC -!- FUNCTION: Required for nuclear protein import and mediates docking of
CC import substrate to distinct nucleoporins (PubMed:11102382). In
CC Drosophila, may not function as a snRNP import receptor as it does not
CC interact with components of the snRNP complex such as snRNP U1, U2,
CC U4/U6 and Snup (PubMed:23885126). {ECO:0000269|PubMed:11102382,
CC ECO:0000269|PubMed:23885126}.
CC -!- SUBUNIT: Forms a complex with an importin alpha subunit (By
CC similarity). Interacts with the sesquiterpenoid juvenile hormone
CC receptor Met (PubMed:27979731). {ECO:0000250|UniProtKB:Q14974,
CC ECO:0000269|PubMed:27979731}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11102382}. Note=In
CC cleavage embryos.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed in embryos.
CC {ECO:0000269|PubMed:11102382}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC {ECO:0000269|PubMed:11102382}.
CC -!- SIMILARITY: Belongs to the importin beta family. Importin beta-1
CC subfamily. {ECO:0000305}.
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DR EMBL; AJ002729; CAA05691.2; -; Genomic_DNA.
DR EMBL; AF222745; AAF34680.1; -; mRNA.
DR EMBL; AE014134; AAF53918.1; -; Genomic_DNA.
DR EMBL; AY118657; AAM50026.1; -; mRNA.
DR RefSeq; NP_001246108.1; NM_001259179.2.
DR RefSeq; NP_001246109.1; NM_001259180.2.
DR RefSeq; NP_001246110.1; NM_001259181.2.
DR RefSeq; NP_001246111.1; NM_001259182.2.
DR RefSeq; NP_477496.1; NM_058148.5.
DR AlphaFoldDB; O18388; -.
DR SMR; O18388; -.
DR BioGRID; 61299; 19.
DR DIP; DIP-23049N; -.
DR IntAct; O18388; 37.
DR MINT; O18388; -.
DR STRING; 7227.FBpp0294035; -.
DR PaxDb; O18388; -.
DR PRIDE; O18388; -.
DR DNASU; 35336; -.
DR EnsemblMetazoa; FBtr0081392; FBpp0080922; FBgn0262743.
DR EnsemblMetazoa; FBtr0305584; FBpp0294035; FBgn0262743.
DR EnsemblMetazoa; FBtr0305585; FBpp0294036; FBgn0262743.
DR EnsemblMetazoa; FBtr0305586; FBpp0294037; FBgn0262743.
DR EnsemblMetazoa; FBtr0305587; FBpp0294038; FBgn0262743.
DR GeneID; 35336; -.
DR KEGG; dme:Dmel_CG2637; -.
DR CTD; 35336; -.
DR FlyBase; FBgn0262743; Fs(2)Ket.
DR VEuPathDB; VectorBase:FBgn0262743; -.
DR eggNOG; KOG1241; Eukaryota.
DR GeneTree; ENSGT00550000074898; -.
DR HOGENOM; CLU_008296_1_0_1; -.
DR InParanoid; O18388; -.
DR OMA; GRICDII; -.
DR OrthoDB; 769199at2759; -.
DR PhylomeDB; O18388; -.
DR Reactome; R-DME-140342; Apoptosis induced DNA fragmentation.
DR Reactome; R-DME-1655829; Regulation of cholesterol biosynthesis by SREBP (SREBF).
DR Reactome; R-DME-6798695; Neutrophil degranulation.
DR Reactome; R-DME-68616; Assembly of the ORC complex at the origin of replication.
DR Reactome; R-DME-909733; Interferon alpha/beta signaling.
DR SignaLink; O18388; -.
DR BioGRID-ORCS; 35336; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 35336; -.
DR PRO; PR:O18388; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0262743; Expressed in oocyte and 32 other tissues.
DR ExpressionAtlas; O18388; baseline and differential.
DR Genevisible; O18388; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005635; C:nuclear envelope; IDA:FlyBase.
DR GO; GO:0005654; C:nucleoplasm; HDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0043186; C:P granule; IPI:FlyBase.
DR GO; GO:0061608; F:nuclear import signal receptor activity; IBA:GO_Central.
DR GO; GO:0008139; F:nuclear localization sequence binding; IBA:GO_Central.
DR GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR GO; GO:0007304; P:chorion-containing eggshell formation; HMP:FlyBase.
DR GO; GO:0000278; P:mitotic cell cycle; HMP:FlyBase.
DR GO; GO:0006607; P:NLS-bearing protein import into nucleus; IDA:FlyBase.
DR GO; GO:0006606; P:protein import into nucleus; IMP:FlyBase.
DR GO; GO:0046822; P:regulation of nucleocytoplasmic transport; IGI:FlyBase.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR021133; HEAT_type_2.
DR InterPro; IPR001494; Importin-beta_N.
DR InterPro; IPR040122; Importin_beta.
DR PANTHER; PTHR10527; PTHR10527; 1.
DR Pfam; PF03810; IBN_N; 1.
DR SMART; SM00913; IBN_N; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50077; HEAT_REPEAT; 1.
DR PROSITE; PS50166; IMPORTIN_B_NT; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Protein transport; Reference proteome; Repeat; Transport.
FT CHAIN 1..884
FT /note="Importin subunit beta"
FT /id="PRO_0000120762"
FT REPEAT 8..37
FT /note="HEAT 1"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT DOMAIN 27..107
FT /note="Importin N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00115"
FT REPEAT 39..71
FT /note="HEAT 2"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 91..129
FT /note="HEAT 3"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 135..166
FT /note="HEAT 4"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 176..208
FT /note="HEAT 5"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 218..253
FT /note="HEAT 6"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 259..308
FT /note="HEAT 7"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 320..366
FT /note="HEAT 8"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 370..400
FT /note="HEAT 9"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 408..444
FT /note="HEAT 10"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 455..491
FT /note="HEAT 11"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 505..542
FT /note="HEAT 12"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 549..597
FT /note="HEAT 13"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 605..645
FT /note="HEAT 14"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 650..687
FT /note="HEAT 15"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 692..730
FT /note="HEAT 16"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 738..782
FT /note="HEAT 17"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 792..835
FT /note="HEAT 18"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 837..878
FT /note="HEAT 19"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REGION 335..348
FT /note="IAB"
FT /evidence="ECO:0000250"
FT REGION 340..425
FT /note="Ran-GTP binding"
FT /evidence="ECO:0000250"
SQ SEQUENCE 884 AA; 98696 MW; DE6DACB927B314EC CRC64;
MTSDIAMQLI AILEKTVSPD KNELLSAKNF LEQAAASNLP EFLKALSEIL VNTANSAVAR
MAAGLQLKNH LTSKDEKVSQ QYQDRWHQFP SEIRELIKNN ILAALGTENT RPSCAAQCVA
YVAVIELPIN RWPMLIQTLV NKVVSEGSSE MHRESALEAI GYICQDIRFG VMENQSNDVL
TAIIHGMRKV EPSNHVRLAA TTALHNSLEF TKSNFEKDME RNFIMEVVCE ATQCQDSQIC
VAALQCLVKI MTLYYQYMEP YMAQALFPIT LAAMKSDNDA VALQGIEFWS NVCDEEIDLA
IESQEATDQG RAPQRVSKHY ARGALQFLTP VLVEKLTKQD ECDDEDTWSP AKAASVCLMV
LATCCEDEIV PHVLPFIKEN IESPNWRFRD AAVMTFGSVL NGLETNTLKP LVEQAMPTLI
RLMYDSSVIV RDTIAWTFGR ICDIIPEAAI NETYLQTLLE CFVKSLKSEP RVAANVCWAF
IGLSDAAWEA AVTNDGETPE TYALSPYFEY IITQLLETTD RSDGAQANLR CAAYQALMDM
IKNSPLDCYL VVQRTTLVIL ERLNQVMQME TQINNHSDRH QFNDLQSLLC ATLQSVLRKV
HEQDAPQISD AIMTALLTMF NSSAGKSGVV QEEAFLAVST LVELLGAQFA KYMPAFKDFL
VMGLKNFQEY QVCCAAVGLT GDIFRALKDL MVPYSNEIMT VLINNLTEPT IHRTVKPQVL
SAFGDIALSI GNHFLPYLSM VLDMLRVASN LQTDANNFDM NEYINELRES ILEAYTGIIQ
GLKGVDQTAH TDVMHMEPHL MHIISFIKRI AQEGDVSDSM LASAAGFIGD LCTSFGPRLY
PLLDDAIITQ FLAEGKRSKA QRTKMLCTWA VKEIKKINTQ VITQ
