IMB_XENLA
ID IMB_XENLA Reviewed; 876 AA.
AC P52297; B0LM40;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 04-FEB-2015, sequence version 3.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Importin subunit beta {ECO:0000303|PubMed:18366719};
DE AltName: Full=Importin-90 {ECO:0000303|PubMed:7627554};
DE AltName: Full=Karyopherin subunit beta {ECO:0000303|PubMed:7878057};
DE AltName: Full=p97 {ECO:0000303|PubMed:7878057};
GN Name=kpnb1 {ECO:0000250|UniProtKB:Q14974};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=18366719; DOI=10.1186/1471-2121-9-14;
RA Delmar V.A., Chan R.C., Forbes D.J.;
RT "Xenopus importin beta validates human importin beta as a cell cycle
RT negative regulator.";
RL BMC Cell Biol. 9:14-14(2008).
RN [2]
RP PARTIAL PROTEIN SEQUENCE, AND INTERACTION WITH IMPORTIN ALPHA.
RC TISSUE=Egg;
RX PubMed=7627554; DOI=10.1016/s0960-9822(95)00079-0;
RA Goerlich D., Kostka S., Kraft R., Dingwall C., Laskey R.A., Hartmann E.,
RA Prehn S.;
RT "Two different subunits of importin cooperate to recognize nuclear
RT localization signals and bind them to the nuclear envelope.";
RL Curr. Biol. 5:383-392(1995).
RN [3]
RP PROTEIN SEQUENCE OF 75-92 AND 184-206, FUNCTION, AND INTERACTION WITH
RP IMPORTIN ALPHA.
RC TISSUE=Ovary;
RX PubMed=7878057; DOI=10.1073/pnas.92.5.1769;
RA Radu A., Blobel G., Moore M.M.;
RT "Identification of a protein complex that is required for nuclear protein
RT import and mediates docking of import substrate to distinct nucleoporins.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:1769-1773(1995).
CC -!- FUNCTION: Required for nuclear protein import and mediates docking of
CC import substrate to distinct nucleoporins.
CC {ECO:0000269|PubMed:7878057}.
CC -!- SUBUNIT: Forms a complex with an importin alpha subunit.
CC {ECO:0000269|PubMed:7627554}.
CC -!- INTERACTION:
CC P52297; Q7ZZY7: rae1.S; NbExp=4; IntAct=EBI-618940, EBI-618949;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q14974}. Nucleus
CC envelope {ECO:0000250|UniProtKB:Q14974}.
CC -!- SIMILARITY: Belongs to the importin beta family. Importin beta-1
CC subfamily. {ECO:0000305}.
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DR EMBL; EU286786; ABY76052.1; -; mRNA.
DR RefSeq; NP_001108315.1; NM_001114843.1.
DR AlphaFoldDB; P52297; -.
DR SMR; P52297; -.
DR IntAct; P52297; 9.
DR MINT; P52297; -.
DR PRIDE; P52297; -.
DR GeneID; 100137718; -.
DR KEGG; xla:100137718; -.
DR CTD; 100137718; -.
DR Xenbase; XB-GENE-994071; kpnb1.S.
DR OrthoDB; 769199at2759; -.
DR Proteomes; UP000186698; Chromosome 9_10S.
DR Bgee; 100137718; Expressed in ovary and 19 other tissues.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005635; C:nuclear envelope; IEA:UniProtKB-SubCell.
DR GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR GO; GO:0006606; P:protein import into nucleus; IEA:InterPro.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR021133; HEAT_type_2.
DR InterPro; IPR001494; Importin-beta_N.
DR InterPro; IPR040122; Importin_beta.
DR PANTHER; PTHR10527; PTHR10527; 1.
DR Pfam; PF03810; IBN_N; 1.
DR SMART; SM00185; ARM; 3.
DR SMART; SM00913; IBN_N; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50077; HEAT_REPEAT; 2.
DR PROSITE; PS50166; IMPORTIN_B_NT; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; Nucleus; Protein transport;
KW Reference proteome; Repeat; Transport.
FT CHAIN 1..876
FT /note="Importin subunit beta"
FT /id="PRO_0000120761"
FT REPEAT 2..31
FT /note="HEAT 1"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT DOMAIN 21..101
FT /note="Importin N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00115"
FT REPEAT 33..65
FT /note="HEAT 2"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 85..123
FT /note="HEAT 3"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 129..160
FT /note="HEAT 4"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 170..202
FT /note="HEAT 5"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 212..247
FT /note="HEAT 6"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 253..302
FT /note="HEAT 7"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 314..360
FT /note="HEAT 8"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 364..394
FT /note="HEAT 9"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 402..438
FT /note="HEAT 10"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 449..485
FT /note="HEAT 11"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 500..537
FT /note="HEAT 12"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 544..592
FT /note="HEAT 13"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 600..639
FT /note="HEAT 14"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 644..681
FT /note="HEAT 15"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 686..724
FT /note="HEAT 16"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 732..776
FT /note="HEAT 17"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 786..829
FT /note="HEAT 18"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REPEAT 831..873
FT /note="HEAT 19"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REGION 329..342
FT /note="IAB-binding"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT REGION 334..419
FT /note="Ran-GTP binding"
FT /evidence="ECO:0000250|UniProtKB:Q14974"
FT CONFLICT 16
FT /note="N -> R (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 86
FT /note="S -> N (in Ref. 2; AA sequence and 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 88
FT /note="R -> P (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 155
FT /note="G -> D (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 315
FT /note="A -> E (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 384
FT /note="D -> Y (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 750
FT /note="S -> G (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 796
FT /note="F -> R (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 869..870
FT /note="LR -> DV (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 876 AA; 97186 MW; 2F8FAACECFFA3AD9 CRC64;
MELVTILEKT VSPDRNELEA AQKFLEQAAV ENLPTFVVEL SKVLANPANS QVARVAAGLQ
IKNPLTSRDP DVKAQYQQRW LAIDASARGE IKTYVLRTLG TESYRPSSAS QCVAGIACAE
ITVNQWPQLI PQLVANVTDP NSTERMKEST LEAIGYICQD IDPEQLQHKS NEILTAIIQG
MRKEEPSNNV RLAATNALLN SLEFTKANFD KESERHYIMQ VVCEATQCPD TRVRVAALQN
LVKIMSLYYQ YMETYMGPAL FAITVEAMKN EIDEVALQGI EFWSNVCDEE MDLAIEASEA
AEQGRPPEHT SKFYAKGALQ YLVPILTQTL TKQDENDDDD DWNPCKAAGV CLMLLATCCE
DDIVPHVLPF IKEHIKNPDW RYRDAAVMAF GCILEGPESC QLKPLVIQAM PTLIELMKDP
SVVVRDTTAW TVGRICELLP EAAINDVYLA PLLQCLIEGL GAEPRVASNV CWAFSSLAEA
AYEAADVADD QEEPSSYCLS SSFEVIVQKL LETTDRPDGH QNNLRSAAYE ALMEIVKNSA
KDCYPAVQKT TLVIMERLQQ VLQVESHIQS TSDRIQFNDL QSLLCATLQN VLRKVQHQDA
LQISDVVMAS LLRMFQSTAG SGGVQEDALM AVSTLVEVLG AEFLKYMEAF KPFLTIGLKN
YAEYQVCLAA VGLVGDLCRA LQSNILPFCD EMMQFLLENL GNENVHRSVK PQILSVFGDV
ALAIGGEFKK YLDVVLNTLQ QASQAQVDKS DYDMVDYLNE LREGCIEAYT GIIQGLKGDQ
ENVHPDVMLV QPRVEFILSF IDHIAGDEDH TDSVVACGAG LIGDLCTAFG KDVLKLVEAR
PMIHELLTEG RRSKTNKTKT LATWATKELR KLKNQA
