IMCE_ORYSJ
ID IMCE_ORYSJ Reviewed; 414 AA.
AC Q6L5F5; A0A0P0WQP7;
DT 27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Probable isoprenylcysteine alpha-carbonyl methylesterase ICME;
DE EC=3.1.1.n2;
GN Name=IMCE; OrderedLocusNames=Os05g0577200, LOC_Os05g50170;
GN ORFNames=OJ1126_B10.7;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Catalyzes the demethylation of isoprenylcysteine
CC methylesters. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl
CC ester + H2O = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine +
CC H(+) + methanol; Xref=Rhea:RHEA:48520, Rhea:RHEA-COMP:12125,
CC Rhea:RHEA-COMP:12126, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17790, ChEBI:CHEBI:90510, ChEBI:CHEBI:90511; EC=3.1.1.n2;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane. Golgi apparatus
CC membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Isoprenylcysteine
CC methylesterase family. {ECO:0000305}.
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DR EMBL; AC098571; AAT39150.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF18320.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS95491.1; -; Genomic_DNA.
DR EMBL; AK069756; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015639665.1; XM_015784179.1.
DR RefSeq; XP_015639666.1; XM_015784180.1.
DR RefSeq; XP_015639667.1; XM_015784181.1.
DR RefSeq; XP_015639668.1; XM_015784182.1.
DR AlphaFoldDB; Q6L5F5; -.
DR SMR; Q6L5F5; -.
DR STRING; 4530.OS05T0577200-01; -.
DR ESTHER; orysa-Q6L5F5; Hormone-sensitive_lipase_like.
DR PaxDb; Q6L5F5; -.
DR PRIDE; Q6L5F5; -.
DR EnsemblPlants; Os05t0577200-01; Os05t0577200-01; Os05g0577200.
DR GeneID; 4339700; -.
DR Gramene; Os05t0577200-01; Os05t0577200-01; Os05g0577200.
DR KEGG; osa:4339700; -.
DR eggNOG; KOG1516; Eukaryota.
DR HOGENOM; CLU_012494_2_4_1; -.
DR InParanoid; Q6L5F5; -.
DR OMA; TDVIVAC; -.
DR OrthoDB; 992858at2759; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR Genevisible; Q6L5F5; OS.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0010296; F:prenylcysteine methylesterase activity; IBA:GO_Central.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR013094; AB_hydrolase_3.
DR Pfam; PF07859; Abhydrolase_3; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Golgi apparatus; Hydrolase; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..414
FT /note="Probable isoprenylcysteine alpha-carbonyl
FT methylesterase ICME"
FT /id="PRO_0000411671"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 223
FT /evidence="ECO:0000305"
FT ACT_SITE 323
FT /evidence="ECO:0000305"
FT ACT_SITE 355
FT /evidence="ECO:0000305"
FT BINDING 151..153
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT BINDING 222..224
FT /ligand="substrate"
FT /evidence="ECO:0000255"
SQ SEQUENCE 414 AA; 45334 MW; 7019CDD56A711C7B CRC64;
MQPASPVSGD AGPVAEAVPP RGAPQVLVRR RSVPFSPDSP LAPGSRGGGE RRSTFREDVS
HAAAETYLVT RLAFILLRYL GVGYRWISQL AALIIYAILL MPGFIRVGYY YFFSRQVLRS
VIYGDQPRNR LDLYIPRDPK KPSPVVAFVT GGAWIIGYKA WGALLGRRLA ERGIIVACID
YRNFPQGTIS DMVSDASDGI SFVCETVGAY GGDPNQIYLM GQSAGAHIAA CALLEQAAKE
SRGEQISWSV TQIKAYFGLS GGYNIENLVD HFHERGLYRS IFLSIMEGKK SLPHFSPETV
AKKLCPETIA LLPQIVLLHG TDDYSIPFSA SETFAGVLKQ AGAKAKLLLY EGKTHTDVFL
QDPLRGGRDK LVEDVISVIH ADDADAREKD ALAPIPGRLV SEWQIKLAHR ISPF
