ID   IMCL1_ORYSJ             Reviewed;         425 AA.
AC   Q5Z9I2; A0A0P0X106;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Probable isoprenylcysteine alpha-carbonyl methylesterase ICMEL1;
DE            EC=3.1.1.n2;
DE   AltName: Full=Isoprenylcysteine methylesterase-like protein 1;
GN   Name=IMCEL1; OrderedLocusNames=Os06g0708100, LOC_Os06g49440;
GN   ORFNames=OsJ_22610, P0621D05.41-1, P0655A07.1-1;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Catalyzes the demethylation of isoprenylcysteine
CC       methylesters. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl
CC         ester + H2O = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine +
CC         H(+) + methanol; Xref=Rhea:RHEA:48520, Rhea:RHEA-COMP:12125,
CC         Rhea:RHEA-COMP:12126, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17790, ChEBI:CHEBI:90510, ChEBI:CHEBI:90511; EC=3.1.1.n2;
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane. Golgi apparatus
CC       membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Isoprenylcysteine
CC       methylesterase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP003621; BAD53618.1; -; Genomic_DNA.
DR   EMBL; AP003634; BAD53625.1; -; Genomic_DNA.
DR   EMBL; AP008212; BAF20445.1; -; Genomic_DNA.
DR   EMBL; AP014962; BAS99428.1; -; Genomic_DNA.
DR   EMBL; CM000143; EAZ38235.1; -; Genomic_DNA.
DR   EMBL; AK102365; BAG95517.1; -; mRNA.
DR   RefSeq; XP_015643069.1; XM_015787583.1.
DR   AlphaFoldDB; Q5Z9I2; -.
DR   SMR; Q5Z9I2; -.
DR   STRING; 4530.OS06T0708100-01; -.
DR   ESTHER; orysa-q5z9i2; Hormone-sensitive_lipase_like.
DR   PaxDb; Q5Z9I2; -.
DR   PRIDE; Q5Z9I2; -.
DR   EnsemblPlants; Os06t0708100-01; Os06t0708100-01; Os06g0708100.
DR   GeneID; 4342018; -.
DR   Gramene; Os06t0708100-01; Os06t0708100-01; Os06g0708100.
DR   KEGG; osa:4342018; -.
DR   eggNOG; KOG1516; Eukaryota.
DR   HOGENOM; CLU_012494_2_4_1; -.
DR   InParanoid; Q5Z9I2; -.
DR   OMA; GKDDMFE; -.
DR   OrthoDB; 992858at2759; -.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000007752; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   ExpressionAtlas; Q5Z9I2; baseline and differential.
DR   Genevisible; Q5Z9I2; OS.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0010296; F:prenylcysteine methylesterase activity; IBA:GO_Central.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR013094; AB_hydrolase_3.
DR   Pfam; PF07859; Abhydrolase_3; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Golgi apparatus; Hydrolase; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..425
FT                   /note="Probable isoprenylcysteine alpha-carbonyl
FT                   methylesterase ICMEL1"
FT                   /id="PRO_0000411672"
FT   TRANSMEM        99..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        154..174
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..42
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        232
FT                   /evidence="ECO:0000305"
FT   ACT_SITE        334
FT                   /evidence="ECO:0000305"
FT   ACT_SITE        366
FT                   /evidence="ECO:0000305"
FT   BINDING         160..162
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
FT   BINDING         231..233
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   425 AA;  46758 MW;  8EFF41C1F5986B31 CRC64;
     MQVELADRAA ARPSETGEAP PSSPAAAAAA SAAAEDAPLL PGGGGGVRRR VVVSERFRQR
     SGSFRREVRR AAEETYLLTR LTLILLRYLG IGYRWIRQFL ALCCYTFLLM PGFIQVVYYY
     FFSSQVCRSV VYGEQPRNRL DLYIPTDRTG LKPVVAFVTG GAWIIGYKGW GALLGRRLAE
     RGILVACIDY RNFPQGTIGD MVEDASQGIA FVCNNIASYG GDPERIYLVG QSAGAHIAAC
     TLLHQAIKES GEGDASTWSI AQLKAYFGIS GGYNLLNLVD HFHKRGLYRS IFLSIMEGEE
     SLQKFSPLVM VKDPAARSAV SLLPRIFLFH GTSDYSIPSA ESEAFFDALQ QNGAKADLFL
     YDGKTHTDLF LQDPLRGGRD KLLEEIVTVI HNDNPDTSAQ HLAVPVARRL VPEFMLMLAG
     RVSPF