IMCL2_ORYSJ
ID IMCL2_ORYSJ Reviewed; 338 AA.
AC Q5VNW5; A0A0P0V5T1;
DT 27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Probable isoprenylcysteine alpha-carbonyl methylesterase ICMEL2;
DE EC=3.1.1.n2;
DE AltName: Full=Isoprenylcysteine methylesterase-like protein 2;
GN Name=IMCEL2; OrderedLocusNames=Os01g0642000;
GN ORFNames=P0039G05.24, P0510C12.9;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Catalyzes the demethylation of isoprenylcysteine
CC methylesters. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl
CC ester + H2O = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine +
CC H(+) + methanol; Xref=Rhea:RHEA:48520, Rhea:RHEA-COMP:12125,
CC Rhea:RHEA-COMP:12126, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17790, ChEBI:CHEBI:90510, ChEBI:CHEBI:90511; EC=3.1.1.n2;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane. Golgi apparatus
CC membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Isoprenylcysteine
CC methylesterase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP003725; BAD68743.1; -; Genomic_DNA.
DR EMBL; AP003855; BAD68856.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF05602.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS73372.1; -; Genomic_DNA.
DR EMBL; AK110911; BAG99079.1; -; mRNA.
DR AlphaFoldDB; Q5VNW5; -.
DR SMR; Q5VNW5; -.
DR STRING; 4530.OS01T0642000-01; -.
DR ESTHER; orysj-b9eya5; Hormone-sensitive_lipase_like.
DR PaxDb; Q5VNW5; -.
DR PRIDE; Q5VNW5; -.
DR EnsemblPlants; Os01t0642000-01; Os01t0642000-01; Os01g0642000.
DR Gramene; Os01t0642000-01; Os01t0642000-01; Os01g0642000.
DR eggNOG; KOG1516; Eukaryota.
DR HOGENOM; CLU_012494_2_1_1; -.
DR InParanoid; Q5VNW5; -.
DR OMA; MDHYEHE; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q5VNW5; OS.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0010296; F:prenylcysteine methylesterase activity; IBA:GO_Central.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002018; CarbesteraseB.
DR Pfam; PF00135; COesterase; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Golgi apparatus; Hydrolase; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..338
FT /note="Probable isoprenylcysteine alpha-carbonyl
FT methylesterase ICMEL2"
FT /id="PRO_0000411673"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 218
FT /evidence="ECO:0000305"
FT ACT_SITE 318
FT /evidence="ECO:0000305"
FT BINDING 146..148
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT BINDING 217..219
FT /ligand="substrate"
FT /evidence="ECO:0000255"
SQ SEQUENCE 338 AA; 37159 MW; AFF904AF300D34DB CRC64;
MRPVSSAEEV GALLSRSDSS GRRRRSSPVQ SASPRPAGCG CGGPRRQSSF RDDVGHAASE
TYLVTRLTFS LLQYLGLGYR WMSQLLALTI YAILLMPGFL QVGYYYFFSS QVRRSIVYGE
QPRNRLDLYI PKDINRPCPV VAFVTGGAWI IGYKAWGSLL GRRLAERGII VACIDYRNFP
QGTIGDMVSD ASQGISYVCN NIASYGGDPN RIYLVGQSAG AHIAACALIE QAVKESSGQS
ISWSVTQIKA YFGLSGGYNM HSLVDHFHER GLNRSIFFSI MEGEESLSRY SPEIVVKQSS
SQTIALLPPI VLMHGTEDYS IPSSARFLLM PSADVHLR
